尖锐湿疣两种治疗方法疗效比较

尖锐湿疣又称尖圭湿疣或性病疣。是由乳头瘤病毒感染引起,主要是通过性接触而好发于生殖器或肛门部位。其治疗方法基本采用化学药物、免疫制剂、生物疗法及手术四大类。我院妇科于1998年10月至1999年8月对70例女性尖锐湿疣患者采用50％三氯醋酸局部烧灼加用迪思安(α-2a注射用基因工程干扰素)和单纯采用 50％三氯醋酸局部烧     

绒癌阴道反复出血抢救护理体会

我院妇科1998年至1999年238d内11次抢救一例绒癌阴道大流血患者。现将抢救及护理体会总结以下五点。 1.敏锐的观察能力,熟练的抢救技能,为抢救阴道突然出血赢得了时间。 2.建立通畅的输血、输液管道,是抢救成功的根本保证。 3.合理分工合作,严格地执行无菌技术,是抢救成功的重要环节。     

果酸换肤术治疗黄褐斑的精细化护理干预

目的 研究精细化护理干预运用于果酸换肤术治疗黄褐斑中的价值.方法 方便选择该院2018年6月—2020年1月纳入的65例果酸换肤术治疗黄褐斑的患者,依照随机双盲法分成两组,其中33例接受精细化护理干预,另32例接受常规护理,对两组总有效率进行评价,干预前后分别进行SDS(抑郁自评量表)、认知能力、依从性、SF-36(生...     

如何保护化疗病人的血管

目前,对恶性肿瘤的治疗,除手术、放疗方法外,静脉化疗方法也较为广泛,如何保证医生制定的化疗方案顺利实施,对长期应用静脉化疗药物的病人的血管加以预防性保护,这是护理工作中值得注意的问题,现将我科对480例静脉化疗病人在血管方面的护理体会总结如下: 一、临床资料 1.化疗时造成静脉血管损伤的原因分析:化疗药物的刺激,由于目前我们所应用的化疗药物毒性很强,刺激静脉血管,使静脉血管管腔变窄,出现疮陷、硬化、失去弹性,     

急性昏迷患者80例急诊急救的临床分析

目的:对救治急性昏迷患者的临床体会进行分析。方法:回顾性分析急性就诊的80例急性昏迷患者临床资料。结果:患者经过积极的救治后,根据脑部功能评分,其中死亡患者4例,其中2例死于手术室中,植物生存的患者有8例,出现肢体功能及脑部功能障碍的患者有22例,其余48例患者均康复。结论:对于急性昏迷患者的救治医院内应建立专科一体化的救治措施,为患者救治开通绿色通道,保证患者的救治工作能安全、有效、快速、优质地开展,同时应该给予脑组织充分的氧气供应可减少继发性脑损伤的出现,及时对外伤患者进行开颅手术清除颅内血肿和活化脑组织,有利于解除颅内高压的压迫,缩短手术的时间,可有利于提高患者的成活率。     

雷公藤治疗子宫肌瘤3例报告

我们用雷公藤治疗类风湿性关节炎的过程中,观察到3例合并子宫肌瘤的患者,2例缩小,1例消失,现介绍如下。例1 刘某某,49岁,住院号35661,因类风湿性关节炎合并子宫肌瘤入院。32岁时因月经不规则,量多有血块,曾在某医院诊断子宫肌瘸。近年来加重,出现尿频,自己能在下腹部摸到肿物,曾确定手术治疗,因服强的松推迟手术。住院后查体左下腹部能触及一个鸭卵大小之肿物,B 超探查子宫左侧有一个5.6×8.8cm 低回声结节,边缘完整、清晰,结合病史,确诊子富肌瘤。开始内服雷公藤,每日6片,分3次服,服药后2个月,经量减少。3个月后闭经,     

抗肝肠钙粘连蛋白单克隆抗体对肝癌细胞生物学行为的影响

目的 观察抗肝肠钙粘连蛋白(CDH17)单克隆抗体Lic5对肝癌细胞生物学行为的影响.方法 Western blot和实时定量聚合酶链反应(PCR)检测细胞株MHCC97H、MHCC97L、PLC/PRF/5及MIHA中CDH17的表达.噻唑蓝(MTT)比色法、细胞划痕法、Transwell法及平板克隆法检测Lic5对肝癌细胞生物学行为的影响.结果 CDH17仅在细胞株MHCC97H、MHCC97L中表达,Lic5可结合肝癌细胞表面的CDH17,并抑制CDH17表达.Lic5 50mg/L组、100mg/L组、小鼠IgG组4 d细胞增殖抑制率在MHCC97H为26.1%、43.6%、6.4%,MHCC97L为26.0%、40.7%、7.7%;Lic5100mg/L组、小鼠IgG组、磷酸盐缓冲液(PBS)组48h细胞迁移抑制率在MHCC97H为36.7%、8.4%、5.6%,MHCC97L为42.3%、10.2%、7.4%(P＜0.05);穿膜细胞数在MHCC97H为(39.20±9.56)、(106.50±7.56)、(96.60±13.02)个,MHCC97L为(26.00±8.61)、(86.00±10.26)、(90.40±12.04)(P＜0.05);克隆形成数在MHCC97H为(59.30±11.68)、(141.70±19.40)、(150.30±14.64),MHCC97L为(57.20±10.21)、(132.50±9.07)、(121.70±11.93)(P＜0.01).Lic5对PLC/PRF/5及MIHA细胞的生物学行为无明显影响.结论 单克隆抗体Lic5能够下调肝癌细胞CDH17表达,抑制肝癌细胞的增殖、迁移、侵袭和克隆形成能力.

Abstract：

Objective To investigate the effect of monoclonal antibody against liver-intestine cadherin (CDH17) on the cell proliferation, migration, invasion and colony formation of hepatocellular carcinoma cell lines. Methods Hepatocellular carcinoma cell lines MHCC97H, MHCC97L, PLC/PRF/5 and MIHA were examined for CDH17 expression by Western blotting and quantitative real-time polymerase chain reaction (PGR). The combination capacity between bepatocellular carcinoma cell lines and monoclonal antibody Lic5 was detected by the way of immunofluorescence staining. The cell lines were treated with Lic5, PBS and mouse IgG respectively. Methyl thiazol tetrazolium (MTT) assay, wound healing assay, Transwell invasion assay and colony formation assay were used to study the changes in cell proliferation, migration, invasion and colony formation of hepatocellular carcinoma cell lines. Results High expression level of CDH17 was detected in MHCC97H and MHCC97L cell lines. CDH17 protein level was down-regulated but there was no significant effect on CDH17 mRNA after treatment with Lie5 in MHCC97H and MHCC97L. Cellular growth rate of MHCC97H in Lic5 (50 mg/L), Lic5 ( 100 mg/L) and mouse IgG groups was decreased by 26. 1%, 43.6% and 6. 4%, and by 26. 0%, 40. 7% and 7. 7% in MHCC97L on the 4th day respectively (P ＜0. 05 ). The inhibition rate of cell migration at 48 h was 36. 7%, 8. 4% and 5.6% in Lic5 ( 100 mg/L), mouse IgG and PBS groups in MHCC97H, and 42. 3%, 10. 2% and 7. 4% in MHCC97L respectively ( P ＜ 0. 05 ). The number of invasion cells was ( 39. 20 t 9. 56),(106.50±7.56) and (96.60±13.02) in MHCC97H, and (26.00±8.61), (86.00±10.26) and (90.40±12.04) in MHCC97L in Lic5 (50 mg/L), Lic5 (100 mg/L) and mouse IgG groups, respectively (P ＜ 0. 05 ). The number of colony formation was ( 59. 30 ± 11.68 ), ( 141.70 ± 19. 40 ) and (150.30 ±14.64) in MHCC97H, and (57.20 ± 10.21), (132.50 ±9.07) and (121.70 ±11.93) in MHCC97L in Lie5 (50 mg/L), Lic5 (100 mg/L) and mouse IgG groups, respectively (P＜ 0. 01 ).There was no significant difference between Lic5 treatment groups and controls in PLC/PRF/5 and MIHA cell lines. Conclusion The anti-CDH17 monoclonal antibody Lic5 can down-regulate CDH17 expression and inhibit the cell proliferation, migration, invasion and colony formation in hepatocellular carcinoma cell lines.