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ifferentialdisplayreversetranscription polymerasechainreaction (DDRT PCR )ofmRNAisarecentlydevelopedtechniquewhichallowstheidentificationandmolecularcloningofgenesdifferentiallyexpressedattwostatesinagiventissue 1 Thedisruptionofwhole bodyglucosehomeosta… 相似文献
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《中国处方药》杂志第80期的“FDA华人系列”专题当中郑慧敏(Jenny H.Zheng)博士的文章——《21世纪的临床药理学:趋向个体化》提及的临床药理学及美国FDA临床药理部在指导药物开发过程的作用,读毕让人深受启发。在中国,不但在药物的开发上缺少这种个体化的规划,即便是在药物的临床使用上也缺乏这种针对病人个体状况的个性化给药, 相似文献
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目的:探讨染色体9p21区域D9S319座位的杂合性缺失(LOH)在胶质瘤的发生发展中的作用。方法:应用微卫星多态标记-PCR法对25例胶质瘤组织进行检测。结果:21例可提供LOH分析信息,杂合性缺失率为57%(12/21),其中胶质母细胞瘤、间变型星形细胞瘤、星形细胞瘤分别为70%(7/10)、43%(3/7)、50%(2/4)。结论:染色体9p21区域杂合性缺失与胶质瘤发生发展密切相关,该区域可能存在抑瘤基因,抑癌基因失活将影响胶质瘤的发生发展。 相似文献
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疾病相关基因的研究策略,方法和技术 总被引:3,自引:0,他引:3
疾病相关基因的研究策略、方法和技术朱国志,方福德(中国医学科学院中国协和医材大学基础医学研究所,北京100005)我们还个能圆满地解释许多与人类健康和疾病有关的现象。对于癌症。心血管疾病,神经/精神疾病和发育疾病等许多严重威胁人类健康的疾病,目前还不... 相似文献
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On the base of the one-step, oparator-independent method which was set up by Christophe A. E., the pancreas was infused with cold University of Wisconsin(UW) solution for the preservation, digested hy the collagenase P, circuited with HBSS 5?tal calf serum(FCS) 10mmol/L Hepes solution, and separated with the stainless steel mesh. The number of the collected islets were 400 000~1 800 000 per pancreas,i.e. about 12 150/g pancreas. After purification, the recovery was 350 000~l 700 000 per pancreas, i, e.about 10 250/g pancreas, the recovery rate was above 80%, and the purity of the final preparation was above 95%. The insnlin secretion in the response to the high concentration glucose (22mmol/L) stimulation was apparently different on the 1,3,5 day of the cultural islets, which the high level of insulin was three times the low level (5.5mmol/L) on the 5th day, and the insulin level of the double stimulation under perfusion conditions is apparently higher than low glucose, The result demonstrated that the purified islets were functionally alive. Histological studies also sllow that the shape of islets are complete, and the β-cell was specially stained by the dithizone (DTZ). The Trypan Blue staining had shown the living cell was above 90%. In conclusion, the new method was highly practical and yielded higher concentration of active pancreatic islets. 相似文献
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