β—2—微球蛋白作为膀胱癌的一种分化标志

正常细胞经增生到恶性细胞,通常与胞核、胞浆及细胞表面内的分子水平的变化有关。这些变化可从正常细胞表面抗原如血型ABH抗原和主要组织相容性复合抗原等的丢失监测到。本文对一组确诊为正常膀胱粘膜、严重化生、乳头状癌、浸润癌、原位癌患者细胞表面β—2—微球蛋白的变化情况进行了研究。方法为对21例患者标本常规石蜡切片,作组织学检查观察形态学变化;平行切片作β—2—微球蛋白免疫过氧化酶组化染色,观察β—2—微球蛋白的存     

硫酸巯基乙烷钠延缓N—亚硝基—N—丁基—(4—羟基丁基)胺诱发大鼠膀胱癌的发生

本文研究了硫酸巯基乙烷钠(Mesna)对N—亚硝基—N—丁基—(4—羟基丁基)胺(BBNOH)诱发大鼠膀胱癌的影响。240只雄性SD大鼠,平均年龄100±5天,常规饲养,随机分成六组。第一、二组(每组45只)为大剂量BBNOH实验组,每公斤体重给药44毫克,BBNOH配成4.4％的混悬液由胃管给药,每周二次。第三、四组(每组45只)为小剂量BBNOH实验组,每公斤体重给药28毫克,配成2.8％混悬液同样方法给药,每周二次。第五、六组(每     

单剂量治疗妊娠菌尿

妊娠期尿中氨基酸、维生素和其它营养物质增多,有利于细菌生长,加上机体抵抗力降低,妊娠菌尿发生率增高。妊娠菌尿的诊断受许多因素影响。留中段尿标本(MSU)作检查易受污染,造成假阳性。本文报导对采取尿标本的方法进行改进,减少了假阳性和提高了准确性、并使用单剂量先锋霉素Ⅳ治疗妊娠菌尿,疗效显著、副作用少。所有病例在首次产前检查时,留取新鲜MSU,经浸片法(dip-slide)筛选菌尿,每ml尿含菌量≥10~5者,进一步作耻骨上抽尿液标本(SPA)培养和药敏确诊。如膀胱充盈不当或太胖不适SPA,则无菌导尿(CSU)作培养和药敏。确诊后可采用单剂量或三天治疗方案。单剂量治疗组:     

阿尔新兰法使乙二醇异丁烯酸酯包埋切片粘固

乙二醇异丁烯酸酮(glycol methacrylate)包埋的切片,在作组织化学和免疫组织化学操作过程中,特别是组织块大而又坚硬的组织切片作染色和切片经过酒精或蛋白酶消化肘,切片易从玻片掉脱下来,这是一个普遍存在的问题。本文作者介绍使用阿尔新兰法(Alcian blue)可解决这一困难。作者将未脱钙的骨髓活检标本,用乙二醇异丁烯酸酯包埋,再用自动切片机将基切为2～3微米     

ESTABLISHMENT OF A NOVEL CELL LINE (HNE_(1)) DERIVED FROM A NASOPHARYNGEAL CARCINOMA

A novel epithelial cell line, designated HNE1 was established from a biopsy specimen of a naso-pharyngeal carcinoma (NPC). Electron microscopic examination of the HNE1 cells demonstrated bi-directional differentiation, with some cells displaying features of poorly differentiated squamous cell carcinoma, while other cells appeared to have the morphology of poorly differentiated adenocarcinoma. The HNE1 cell line has been passaged more than 100 times over a period of one year. We recently reported that the Epstein-Barr virus (EBV) nuclear antigen (EBNA) was detected in a low pe rcentage of the HNE1 cells examined at subcultures 5-81; the cells were also shown to be EBV DNA positive. Tumorigenicity of the HNE1 cells was demonstrated by xentransplanta tion in athymic nude mice. The developed tumors were characterized as well-differentiated squamous cell carcinomas upon histological examination. Kar yotypic analysis of the HNE1 cells demonstrated an aneuploidy with a modal chromosomal number of 74 at passage