老年人静脉血细胞分析参考值范围的调查研究

Objective To establish the reference range of venous blood cells of healthy aged people by means of investigation on the reference range of venous blood cells of healthy adults and the aged.Methods The parameters of 3302 venous blood samples from the healthy adults and the aged were analyzed by applying SymexKX-21N hematology analyzer,and the test results were statistically analyzed with SPSS10.0 software.Results The reference range of the healthy aged people(males and females):WBC(3.57-9.00)×109/L and(3.52-8.39)×109/L;RBC(3.78-5.42)×1012/L and (3.44-4.83)×1012/L;hemoglobin(Hb)121.40-168.10 and 107.90-147.90 g/L;hematocrit(HCT)36.62%-49.18% and 32.82%-44.03%;mean corpuscular volume(MCV)85.10-100.60 and 84.70-100.60 fL;mean corpuscular hemoglobin(MCH)28.54-34.48 and 28.20-34.10 pg;mean corpuscular hemoglobin concentration(MCHC)322.00-357.00 and 320.00-352.00 pg/L;coefficient of variation of red blood cell distribution width(RDW-CV)12.13%-15.10% and 10.20%-17.12%;platelet(PLT)(70.00-240.00)×109/L and(77.00-262.00)×109/L;mean platelet volume(MPV)9.77-15.37 and 9.51-15.55 fL;platelet distribution width(PDW)11.44%-27.77%;platelet hematocrit(PCT)1.10-2.73 and 1.13-2.99 mL/L;large platelet ratio(P-LCR)24.35%-70.14%.The differences of the parameters(WBC,RBC,Hb,HCT,MCHC,PLT and PCT)were statistically significant between senile males and senile females(P＜0.01),while the other parameters weren't(P＞0.05);the differences of parameters(WBC,MPV,PDW and P-LCR)were not significant between adults and the ageds(P＞0.05),while the other parameters were(P＜0.05).There were also significant differences(P＜0.05)between the test results and the reported date about the blood cell parameters of adults and the ageds.Conclusion The reference range of the healthy ageds is different from that of adults;therefore it is necessary to establish the laboratory reference range of venous blood cells of the elderly in this region scientifically and rationally.     

老年人静脉血细胞分析参考值范围的调查研究

Objective To establish the reference range of venous blood cells of healthy aged people by means of investigation on the reference range of venous blood cells of healthy adults and the aged.Methods The parameters of 3302 venous blood samples from the healthy adults and the aged were analyzed by applying SymexKX-21N hematology analyzer,and the test results were statistically analyzed with SPSS10.0 software.Results The reference range of the healthy aged people(males and females):WBC(3.57-9.00)×109/L and(3.52-8.39)×109/L;RBC(3.78-5.42)×1012/L and (3.44-4.83)×1012/L;hemoglobin(Hb)121.40-168.10 and 107.90-147.90 g/L;hematocrit(HCT)36.62%-49.18% and 32.82%-44.03%;mean corpuscular volume(MCV)85.10-100.60 and 84.70-100.60 fL;mean corpuscular hemoglobin(MCH)28.54-34.48 and 28.20-34.10 pg;mean corpuscular hemoglobin concentration(MCHC)322.00-357.00 and 320.00-352.00 pg/L;coefficient of variation of red blood cell distribution width(RDW-CV)12.13%-15.10% and 10.20%-17.12%;platelet(PLT)(70.00-240.00)×109/L and(77.00-262.00)×109/L;mean platelet volume(MPV)9.77-15.37 and 9.51-15.55 fL;platelet distribution width(PDW)11.44%-27.77%;platelet hematocrit(PCT)1.10-2.73 and 1.13-2.99 mL/L;large platelet ratio(P-LCR)24.35%-70.14%.The differences of the parameters(WBC,RBC,Hb,HCT,MCHC,PLT and PCT)were statistically significant between senile males and senile females(P＜0.01),while the other parameters weren't(P＞0.05);the differences of parameters(WBC,MPV,PDW and P-LCR)were not significant between adults and the ageds(P＞0.05),while the other parameters were(P＜0.05).There were also significant differences(P＜0.05)between the test results and the reported date about the blood cell parameters of adults and the ageds.Conclusion The reference range of the healthy ageds is different from that of adults;therefore it is necessary to establish the laboratory reference range of venous blood cells of the elderly in this region scientifically and rationally.     

老年人静脉血细胞分析参考值范围的调查研究

Objective To establish the reference range of venous blood cells of healthy aged people by means of investigation on the reference range of venous blood cells of healthy adults and the aged.Methods The parameters of 3302 venous blood samples from the healthy adults and the aged were analyzed by applying SymexKX-21N hematology analyzer,and the test results were statistically analyzed with SPSS10.0 software.Results The reference range of the healthy aged people(males and females):WBC(3.57-9.00)×109/L and(3.52-8.39)×109/L;RBC(3.78-5.42)×1012/L and (3.44-4.83)×1012/L;hemoglobin(Hb)121.40-168.10 and 107.90-147.90 g/L;hematocrit(HCT)36.62%-49.18% and 32.82%-44.03%;mean corpuscular volume(MCV)85.10-100.60 and 84.70-100.60 fL;mean corpuscular hemoglobin(MCH)28.54-34.48 and 28.20-34.10 pg;mean corpuscular hemoglobin concentration(MCHC)322.00-357.00 and 320.00-352.00 pg/L;coefficient of variation of red blood cell distribution width(RDW-CV)12.13%-15.10% and 10.20%-17.12%;platelet(PLT)(70.00-240.00)×109/L and(77.00-262.00)×109/L;mean platelet volume(MPV)9.77-15.37 and 9.51-15.55 fL;platelet distribution width(PDW)11.44%-27.77%;platelet hematocrit(PCT)1.10-2.73 and 1.13-2.99 mL/L;large platelet ratio(P-LCR)24.35%-70.14%.The differences of the parameters(WBC,RBC,Hb,HCT,MCHC,PLT and PCT)were statistically significant between senile males and senile females(P＜0.01),while the other parameters weren't(P＞0.05);the differences of parameters(WBC,MPV,PDW and P-LCR)were not significant between adults and the ageds(P＞0.05),while the other parameters were(P＜0.05).There were also significant differences(P＜0.05)between the test results and the reported date about the blood cell parameters of adults and the ageds.Conclusion The reference range of the healthy ageds is different from that of adults;therefore it is necessary to establish the laboratory reference range of venous blood cells of the elderly in this region scientifically and rationally.     

嗜醇黄杆菌引起的败血症1例

患者,女,37岁.因高热,颈强直,休克急诊入我院ICU病房抢救.该患者有甲亢病史,1周前有过手指刀伤,入院时体温39.5℃,入院时曾考虑真菌感染性脑膜炎.实验室检查:血WBC8.0×109/L,NO.80,L0.18,E0.02;CSF常规正常,墨汁染色阴性,培养7日无菌生长.     

MVIS-2035全自动血液流变分析仪测定血液流变学指标参考值范围的探讨

目的探讨MVIS-2035全自动血液流变分析仪测定血液流变学指标的参考值范围。方法用MVIS-2035全自动血液流变分析仪测定485例健康体检者的全血高切（200／S）、中切（30／s）、低切（3／s）黏度值，血浆黏度值，红细胞聚集指数，红细胞刚性指数，血液屈服应力和红细胞电泳时间。结果485例健康人血液流变学指标均呈正态分布。除血液屈服应力外各血液流变学指标在不同性之间差别有显著性意义（P〈0．05）。各年龄组之间（〈20岁组分别与与20—39岁组、40～59岁组、／〉60岁组比较）比较：20～39岁组、40～59岁组、红细胞聚集指数增高，血浆黏度值降低。而≥60岁组全血低切黏度值及红细胞聚集指数增高，血浆黏度值降低（P〈0．05），红细胞刚性指数增高（P〈0．05）。95％可信区间正常参考值：男、女组的全血高切黏度值分别是4．81—6．63mPa／s、4．52～6．17mPa／s，全血中切黏度值分别是6．70—8．88mPa／s、6．14～8．35mPafs，全血低切黏度值分别是9．72～12．94mPa／s、8．78～12．18mPa／s，血浆黏度值为1．40～1．72mPafs、1．37—1．84，红细胞聚集指数分别是5．68—8．57、5．19—7．85，红细胞刚性指数分别是0．81～1．11、0．75—1．08，血液屈服应力为0．86—1．35，红细胞电泳时间分别是16．00-21．27s、14．89—19．95s。结论血液流变学的各项实验室检查指标在不同性别、年龄之间存在一定的差异。各实验室应建立自己的参考值为临床诊断提供最可靠的依据。     

脑脊液中炎性指标对细菌性脑膜炎菌株鉴别的意义

目的探讨脑脊液中炎性指标对患者颅内感染菌株鉴别的意义,为临床经验性用药提供参考。方法选取2015年9月至2017年7月重庆市第四人民医院诊断的86例细菌性脑膜炎患者作为观察组,根据感染细菌的不同分为革兰阳性菌组和革兰阴性菌组,另选取因外科手术需要腰麻并无神经系统疾病的患者且肝肾功能正常的人群20例作为对照组,比较观察组与对照组及革兰阳性菌组和革兰阴性菌组脑脊液葡萄糖(GLU)、总蛋白(PRO)、腺苷脱氨酶(ADA)、白细胞计数(WBC)结果。结果观察组脑脊液GLU水平较对照组低,差异有统计学意义(P0.05),其他指标较对照组高,差异有统计学意义(P0.05);革兰阴性菌组脑脊液GLU显著低于革兰阳性菌组,差异有统计学意义(P0.05);革兰阴性菌组脑脊液ADA显著高于革兰阳性菌组(P0.05)。结论检测脑脊液GLU、ADA、WBC、PRO水平对颅内感染菌株有辅助诊断作用,并有利于颅内感染抗菌药物的选择。     

锌指蛋白281对肝癌细胞增殖的影响

目的:研究锌指蛋白281(zinc finger protein 281,ZNF281)对肝癌细胞增殖的作用。方法:用realtime PCR检测80例健康人群及206例肝癌患者外周血单个核细胞中ZNF281的mRNA表达水平,并分析肝癌患者单个核细胞中ZNF281的表达水平与多个临床病理参数的相关性。采用real-time PCR及Western blot实验分别检测ZNF281在肝癌细胞和永生化肝细胞中的mRNA及蛋白表达水平。利用小干扰RNA靶向沉默Huh-7细胞和SKHep-1细胞中ZNF281的表达,然后用MTS法检测肝癌细胞活力的变化;EdU标记实验检测肝癌细胞增殖能力的变化;平板集落形成实验检测肝癌细胞集落形成能力的变化;软琼脂集落形成实验检测肝癌细胞锚定非依赖生长能力的变化。结果:与正常健康人群相比,肝癌患者的外周血单个核细胞中ZNF281的mRNA表达水平明显增高,且其增高水平与肿瘤的大小、分期以及血管侵袭性存在正相关。肝癌细胞中ZNF281表达水平高于永生化肝细胞(P0.05)。沉默ZNF281可以抑制肝癌细胞的活力,抑制肝癌细胞增殖,DNA合成降低,集落数量减少,锚定非依赖性生长能力减弱(P0.05)。结论:ZNF281可促进肝癌细胞的增殖。     

老年人静脉血细胞分析参考值范围的调查研究

Objective To establish the reference range of venous blood cells of healthy aged people by means of investigation on the reference range of venous blood cells of healthy adults and the aged.Methods The parameters of 3302 venous blood samples from the healthy adults and the aged were analyzed by applying SymexKX-21N hematology analyzer,and the test results were statistically analyzed with SPSS10.0 software.Results The reference range of the healthy aged people(males and females):WBC(3.57-9.00)×109/L and(3.52-8.39)×109/L;RBC(3.78-5.42)×1012/L and (3.44-4.83)×1012/L;hemoglobin(Hb)121.40-168.10 and 107.90-147.90 g/L;hematocrit(HCT)36.62%-49.18% and 32.82%-44.03%;mean corpuscular volume(MCV)85.10-100.60 and 84.70-100.60 fL;mean corpuscular hemoglobin(MCH)28.54-34.48 and 28.20-34.10 pg;mean corpuscular hemoglobin concentration(MCHC)322.00-357.00 and 320.00-352.00 pg/L;coefficient of variation of red blood cell distribution width(RDW-CV)12.13%-15.10% and 10.20%-17.12%;platelet(PLT)(70.00-240.00)×109/L and(77.00-262.00)×109/L;mean platelet volume(MPV)9.77-15.37 and 9.51-15.55 fL;platelet distribution width(PDW)11.44%-27.77%;platelet hematocrit(PCT)1.10-2.73 and 1.13-2.99 mL/L;large platelet ratio(P-LCR)24.35%-70.14%.The differences of the parameters(WBC,RBC,Hb,HCT,MCHC,PLT and PCT)were statistically significant between senile males and senile females(P＜0.01),while the other parameters weren't(P＞0.05);the differences of parameters(WBC,MPV,PDW and P-LCR)were not significant between adults and the ageds(P＞0.05),while the other parameters were(P＜0.05).There were also significant differences(P＜0.05)between the test results and the reported date about the blood cell parameters of adults and the ageds.Conclusion The reference range of the healthy ageds is different from that of adults;therefore it is necessary to establish the laboratory reference range of venous blood cells of the elderly in this region scientifically and rationally.     

老年人静脉血细胞分析参考值范围的调查研究

Objective To establish the reference range of venous blood cells of healthy aged people by means of investigation on the reference range of venous blood cells of healthy adults and the aged.Methods The parameters of 3302 venous blood samples from the healthy adults and the aged were analyzed by applying SymexKX-21N hematology analyzer,and the test results were statistically analyzed with SPSS10.0 software.Results The reference range of the healthy aged people(males and females):WBC(3.57-9.00)×109/L and(3.52-8.39)×109/L;RBC(3.78-5.42)×1012/L and (3.44-4.83)×1012/L;hemoglobin(Hb)121.40-168.10 and 107.90-147.90 g/L;hematocrit(HCT)36.62%-49.18% and 32.82%-44.03%;mean corpuscular volume(MCV)85.10-100.60 and 84.70-100.60 fL;mean corpuscular hemoglobin(MCH)28.54-34.48 and 28.20-34.10 pg;mean corpuscular hemoglobin concentration(MCHC)322.00-357.00 and 320.00-352.00 pg/L;coefficient of variation of red blood cell distribution width(RDW-CV)12.13%-15.10% and 10.20%-17.12%;platelet(PLT)(70.00-240.00)×109/L and(77.00-262.00)×109/L;mean platelet volume(MPV)9.77-15.37 and 9.51-15.55 fL;platelet distribution width(PDW)11.44%-27.77%;platelet hematocrit(PCT)1.10-2.73 and 1.13-2.99 mL/L;large platelet ratio(P-LCR)24.35%-70.14%.The differences of the parameters(WBC,RBC,Hb,HCT,MCHC,PLT and PCT)were statistically significant between senile males and senile females(P＜0.01),while the other parameters weren't(P＞0.05);the differences of parameters(WBC,MPV,PDW and P-LCR)were not significant between adults and the ageds(P＞0.05),while the other parameters were(P＜0.05).There were also significant differences(P＜0.05)between the test results and the reported date about the blood cell parameters of adults and the ageds.Conclusion The reference range of the healthy ageds is different from that of adults;therefore it is necessary to establish the laboratory reference range of venous blood cells of the elderly in this region scientifically and rationally.