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丹参毛状根培养的建立和丹参酮的产生 总被引:39,自引:8,他引:39
丹参毛状根培养的建立和丹参酮的产生张荫麟,宋经元,吕桂兰,刘惠灵(中国医学科学院药用植物研究所北京100094)丹参SalviamiltiorrhizaBunge是治疗心血管系统疾病的重要中革药,主要成分如丹参酮具有活血、抗菌等活性,同时也是一种红色色素。利用细胞培养方法来生产这些活性成分,不仅对满足临床用药需要有重要意义,作为一种具有生理活性的色素开发也有良好前景。植物毛状根培养是80年代发展起来的植物基因工程和细胞工程相结合的?... 相似文献
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丹参冠瘿组织的生长和总丹参酮的积累动态 总被引:6,自引:0,他引:6
AIM To determine the dynamics of growth and total tanshinones accumulation in crown gall cultures of Salvia miltiorrhiza in MS and 67-V liquid media. METHODS Fresh, dry weight and total tanshinones yields in the cultures and in the medium were determined every 5 days in crown gall suspension cultures. RESULTS In MS medium, the logarithmic growth phase of crown gall cultures in S.miltiorrhiza was from the 5th to 30th days, and the stationary growth phase was from the 30th to 35th days. From the 25th to 30th days, physiological activity of crown gall cultures was higher and their growth was better. However, in 67-V medium, the logarithmic growth phase of crown gall cultures was from the 10th to 25th days, and the stationary growth phase was from the 25th to 35th days. Total tanshinones were largely accumulated in the cultures and in the medium after 25 days. The total tanshinones yield (60 mg.L-1) was reached at the 35th day. CONCLUSION Knowing the regularity of the growth and total tanshinones accumulation in crown gall cultures of S.miltiorrhiza will be helpful to take proper regulative measures in order to obtain the maximum total tanshinones yield. 相似文献
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迈向二十一世纪的药用植物生物技术 总被引:11,自引:0,他引:11
本文阐述了当今涉及到基因工程,细胞工程,组织、器官培养等几个层次上所进行的药用植物生物技术。同时对细胞培养与活性成分生产,转基因器官培养,转基植物,药用植物有效成分的调控技术等当前药用植物生物技术的研究热点进行了讨论。 相似文献