The determination and significance of C/EBP in cells of liver cancer and different liver tissues

C/EBP is a sequence-specific DNA-binding protein.In order to identify its distribu-tion,localization and function,immunocytochemical technique(ABC method)was done usinganti-C/EBP polypeptide antibodies 1103~#,425~# in liver specimens from 20 normal adult,5neonatal,6 patients with hepatitis,25 patients with liver cirrhosis,80 patients with hepatocellu-lar carcinoma(40 cases were associated with surrounding nontumorous tissues)and 26 patientswith cholangiocarcinoma(15 cases were associated with surrounding nontumorous tissues).Theresults showed that C/EBP was diffusely distributed in nuclei and cytoplasm of differentiated liv-er cells and very low or undetectable in liver cancer cells.The expression of C/EBP was in pro-portion to differentiated degree of tumor cells,and was obviously weaker than that in surround-ing nontumorous tissues.C/EBP positive staining has also been found in regenerating epithelialcells of bile ductules.The results suggested that C/EBP might play an important role in estab-lishing and maintaining the differentiation of liver cells and might exert an inhibiting effect againsttransformation of liver cells and proliferation of neoplastic tissue.     

人肝细胞肝癌与正常肝组织内C／EBP的分布及其mRNA丰度分析

以兔抗增强子结合蛋白（ＣＣＡＡＴ／ＥｎｈａｎｃｅｒＢｉｎｄｉｎｇＰｒｏｔｅｉｎ，Ｃ／ＥＢＰ）多肽抗体，用ＡＢＣ法对１８例人正常肝组织、５例新生儿肝组织及７９例肝细胞肝癌（其中４０例带有癌旁肝组织）中Ｃ／ＥＢＰ进行免疫组化定位，同时运用Ｃ／ＥＢＰｃＤＮＡ探针对３例人正常肝组织、１例新生儿肝组织、１０例肝细胞肝癌（其中８例含配对的癌旁肝组织）进行Ｎｏｒｔｈｅｒｎ杂交分析。免疫组化结果显示，Ｃ／ＥＢＰ弥漫分布于分化成熟的肝细胞浆及肝细胞核中（胞浆含量更丰富），在肝癌分化差（或分化低）的组织细胞中含量低或检测不到，并和癌的分级存在一定相关性。所有增生的胆管上皮细胞也呈阳性。Ｃ／ＥＢＰｍＲＮＡ丰度（表达量）和免疫组化结果基本相符。以上结果进一步证实Ｃ／ＥＢＰ在维持肝细胞分化状态中担负重要作用。     

介绍一种简易眼科手术孔巾

我院以前所用的眼科孔巾和其他孔巾相同,如双眼都行手术,則做完一边手术后需要再换一塊孔巾,这样不仅手續麻煩,延長手术时間,还有碍無菌技术的掌握,因此我們試制了一种双孔帶盖的孔巾。經改进后一年来的經驗証     

用双脱氧终止法直接测定单链PCR产物的序列

用双脱氧终止法直接测定单链ＰＣＲ产物的序列张荣美，王凡，徐砺新（第四军医大学病理学教研室西安７１００３３２第四军医大学西京医院眼科）关键词单链多聚酶链式反应，序列分析中图号Ｑ７４ＰＣＲ技术的发展日新月异，ＰＣＲ技术用于序列分析已为大多数实验室所采用，...