复方环磷酰胺片临床疗效分析报告

抗癌新剂型药-复方环磷酰胺片已经由本溪第三制药厂与辽宁省药物研究所协作研制成功。于1986年1月在辽宁省卫生厅主持下通过了技术鉴定。现已投放市场并且收到较理想的经济效益和社会效益。对该药进行临床观察的有沈阳市第五人民医院、北京市肿瘤研究所、本溪市卫生学校附属医院、辽宁省肿瘤医院等医疗单位。鉴定会前他们已经提出各自的临床报告。报告中指出:复方环磷酰胺与单方环磷酰胺比较,具有保护白细胞和减轻胃肠道反应的作     

五味子醇甲对中枢神经系统单胺类递质的

Schizandrol A (2',3',4',1",2",3"-hexamethoxy-6, 7-dimethyl-1,2,3,4-dibenzo-1,3-cyclooctadien-6-ol) is one of the effective components in the dried fruit of Schizandra chinensis Bail. Previous studies have found that schizandrol A exerts inhibitory effects on the central nervous system (CNS). For the purpose of elucidating the mechanism of inhibition, the concentrations of monoamine neurotransmitters and their metabolites in rat brain and the effects of schizandrol A on some receptors were determined by the ion-pairing reversed-phase liquid chromatography with electrochemical detection method and competitive binding assay. In the neurotransmitter studies, significant elevations of dopamine and its metabolite DOPAC (in striatum) and DA (in hypothalamus) were observed after i.p. administration of 50 mg/kg or 100 mg/kg of schizandrol A. But the receptor binding experiments showed that schizandrol A had no affinity for dopamine D1 and D2 receptors. Serotonin receptors and alpha 1-,alpha 2-adrenergic receptors, and it did not affect the binding of dopamine to dopamine D1 or D2 receptors. These results indicate that the inhibition exerted by schizandrol A on the CNS may be related to the dopamine system, and the increase of dopamine turnover has nothing to do with dopamine receptors. The concentrations of the norepinephrine metabolite MHPG and the serotonin metabolite 5-HIAA showed changes in rat striatum and hypothalamus after schizandrol A treatment, but norepinephrine and serotonin levels were unaffected.     

分光光度法测定米托蒽醌的含量

以乙醇为溶剂，采用分光光度测定米托蒽酯的含量。米托蒽醌浓度在４～１０ｍｇ／Ｌ范围，线性关系良好（ｒ＝０．９９９９），方法的平均回收率为９９．２０％，ＲＳＤ为０．２０％（ｎ＝８）７个不同批号样品的ＲＳＤ为０．０１％～０．６８％，ｎ＝３）。     

用高效液相色谱—电化学检测法同时测定大鼠脑内单胺递质及其主要代谢产物

本文报道用离子对反相液相色谱—电化学检测法同时测定大鼠脑内单胺递质及其代谢产物。本法采用高氯酸沉淀组织蛋白后上清液直接进佯技术,能使NE、E、DA、5-HT及它们的主要代谢产物MHPG、DHPG、HVA、DOPAC、5-HIAA与内标异丙肾上腺奈等十种物质很好地同时分离。由此不仅能测定单胺类神经递质在脑内的含量,并能测定其转换率。方法简便快速,回收率较高且稳定。文中对色谱分离及检测的某些影响因素做了分析讨论,并测定了大鼠下丘脑内单胺递质及其代谢产物。     

五味子醇甲对中枢神经系统单胺类递质的影响

本实验用高效液相色谱—电化学检测法及受体配体结合法观察五味子醇甲对大鼠脑内单胺类递质及其受体的影响,结果表明五味子醇甲可使大鼠纹状体及下丘脑多巴胺含量明显增加,提示五味子醇甲的中枢抑制作用可能与多巴胺系统有一定联系。