SLE患者血浆维生素C、E水平的变化研究

目的：探讨血浆维生素C、E水平与SLE疾病之间的关系及可能采取的相应对策。方法：对33例确诊为SLE患者及对等匹配的正常人的血浆维生素C及维生素E含量进行测定。结果：SLE组血浆维生素C含量为37．98±15．64mol／L,维生素E含量为14．86±6．53mol／L,均明显低于对照组（P＜0．01）。结论：维生素C、E含量检测可作为反映SLE病变严重程度的一组参考指标。     

维生素D受体基因启动子区CDX2多态性与儿童氟斑牙的关系

目的 了解维生素D受体(VDR)基因启动子区CDX2结合位点多态性与儿童氟斑牙易感性的关系.方法 Dean法检查8～12岁风台县关店乡儿童氟斑牙患病情况,收集氟斑牙患者102例及正常对照者107例,采集外周静脉血提取DNA,采用等位基因特异多重聚合酶链反应(ASM-PCR)测定两组儿童VDR基因CDX2结合位点多态性.结果 VDR基因CDX2结合位点多态性分布频率为:氟斑牙患者GG占48.0%、AG占25.5%、AA占26.5%;正常对照组GG占55.1%、AG占28.0%、AA占16.9%.两组基因型分布比较差异无统计学意义(P＞0.05).结论 VDR基因CDX2结合位点多态性与所调查儿童的氟斑牙发生无明显关系.     

CE-TRUS引导前列腺穿刺活检的临床价值

目的 探讨经直肠超声造影在前列腺穿刺活检中的临床价值.方法 选择临床疑诊为前列腺癌预行经直肠前列腺穿刺活检术的68例患者,分为对照组(30例)和造影组(38例),所有患者均采用10点系统穿刺法行前列腺穿刺活检,造影或者常规超声提示可疑病灶处增加1~2针.比较两组患者穿刺病理结果、病理阳性率及两组的敏感性、特异性、准确性.结果 造影组病理阳性率(36.9%)显著高于对照组(27.0%)(P<0.05).经直肠超声造影引导前列腺穿刺活检对诊断前列腺癌的敏感性、特异性和准确性(82.4%、71.4%、76.3%)均显著高于经直肠超声引导的前列腺穿刺活检(64.2%、56.3%、60.0%)(P<0.05).结论 经直肠超声造影引导下前列腺穿刺活检提高前列腺癌的检出率,但由于假阴性和假阳性的存在,经直肠超声造影引导前列腺穿刺仍无法代替系统穿刺.     

胰岛移植治疗糖尿病：附11例报告

我院于1986年11月始开展人胎胰岛细胞培养研究工作,1987年3月起应用于临床,至1988年6月共移植于11例病人(其中2例先后移植2次),均无移植后不良反应,移植后胰岛素用量大多有一定的减少,其中血糖较移植前稳定且降低,糖尿病并发症(除晚期视网膜病变外)均有改善.     

非酮症高渗性昏迷的诊断与治疗

非酮症高渗性昏迷的诊断与治疗浙江医科大学附属第二医院［３１０００９］岳岚，宋作非酮症高渗性昏迷（ＮＫＨＣ）是严重的内分泌综合征，易误诊、漏诊而贻误治疗，死亡率较高。本文报道５年来遇到的１０例非酮症高渗性昏迷者，诱发因素为感染、脑出血、开颅术及心脏直视...     

维生素D受体基因多态性与汉族儿童氟斑牙易感性的关系研究

objective To observe the distribution of vitamin D receptor(VDR)gene polymorphisms in children of Han nationality and investigate the relationship between VDR gene polymorphisms and the susceptibility to children's dental fluorosis of Han nationality.Methods From October of 2008 to March of 2009,a case-control study was conducted among children between 8 and 12 years old with(n=101)and without(n=102)dental fluorosis using Dean method in Guandian countyside of Fengtai county in Anhui province.DNA was extracted from blood samples ofthese children.The Apa I,Bsm I,Fok I and raq I polymorphisms in the VDR gene were genotyped using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The distribution of the genotypes in patients and the control group were analyzed. Results Different genotypes of the VDR gene existed in children of Han nationality with the highest distribution frequency of Aa, bb, Ff, TT, which respectively was 51.7% ( 105/203 ), 89.7% (182/203), 52.7% (107/203), 93.1% (189/203), followed by genotype distribution frequency of aa, Bb, FF, Tt, being 39.9% (81/203),7.9% (16/203),31.5% (64/203) ,6.9% (14/203), respectively. AA,BB, ff, tt distribution frequency was the lowest as follows, 8.4% ( 17/203 ), 2.4% (5/203), 15.8% (32/203),0 (0/203). The frequency distribution of VDR Apa I genotype was AA 7.9% (8/101), Aa 55.4% (56/101), aa 36.7%(37/101) in children with fluorosis, and AA 8.8% (9/102), Aa 48.0% (49/102), aa 43.3% (44/102) in children without fluorosis, respectively. There were no significant differences in the two groups(χ2= 1.13, P > 0.05).The frequency distribution of VDR Bsm I genotype was BB 3.0%(3/101), Bb 5.9%(6/101 ), bb 91.1% (92/101) in children with fluorosis, and BB 2.0% (2/102), Bb 9.8% (10/102), bb 88.2% (90/102) in children without fluorosis, respectively. There were no significant differences in the two groups(χ2 = 0.55, P > 0.05). The frequency distribution of VDR Fok I genotype was FF 28.7%(29/101), Ff 56.4% (57/101), ff 14.9%(15/101) in children with fluorosis, and FF 34.3% (35/102), Ff 49.0% (50/102), ff 16.7% (17/102) in children without fluorosis,respectively. There were no significant differences in the two groups(χ2 = 1.14, P > 0.05). The frequency distribution of VDR Taq I genotype was TT 93.1%(94/101), Tt 6.9%(7/101) in children with fluorosis, and TT 93.1% (95/102), Tt 6.9%(7/102) in children without fluorosis, respectively. The tt genotype was not found. There were no significant differences in the two groups (χ2 = 0.00, P > 0.05 ). Conclusions Different genotypes of the VDR gene existed in children of Han nationality. There were no correlation between VDR Apa I , Bsm I , Fok I , Taq I gene polymorphisms and children's dental fluorosis of Han nationality in this area.     

氦、氖激光对转氨酶与硷性磷酸酶的作用

引言关于弱激光对生物过程的刺激作用,我们曾观察研究了氦、氖激光对淀粉酶、过氧化酶、转氨酶活性的影响。本次实验是用632.8毫微米的氦氖激光器,在相同功率密度、不同能最密度的条件下,照射人体血液中分离出的血清,观察研究经激光照射一般时间后、转氨酶和硷性磷酸酶活性有无影响。及观察研究经氦氖激光照射一天、两天、三天、四天后转氨酶活性有无影响。     

机体抗氧化比值及抗氧化维生素水平与SLE的关系研究

目的 探讨机体抗氧化水平与ＳＬＥ疾病之间的关系及可能采取的相应对策。方法 对３３例确诊为ＳＬＥ患者及对等匹配的正常人的血抗氧化比值、抗坏血酸及α－生育酚含量进行测定。结果 ＳＬＥ患者组血浆抗坏血酸含量为（３７．９８±１５．６４）ｍｏｌ／Ｌ，α－生育酚含量为（１４．８６±６．５３）ｍｏｌ／Ｌ，血浆抗氧化比值为１．６２±０．７３，红细胞抗氧化比值为４８．３５±１４．２２，均明显低于对照组。血浆及细胞抗     

全牙列金属烤瓷固定修复3例的体会

随着现代口腔修复工艺的不断完善，新材料、新技术的使用，要求烤瓷固定修复的病人数量也日益增加。本文就我科近1年半以来制做的3例全牙列金属烤瓷联冠固定修复，谈一些体会。