天然生化适应剂增强人体耐力效应的研究

<正> 通过在体动物实验及体外试验,证明“适应剂”能提高动物机体的耐力和智力、改善心功能等功效。在大量的动物实验基础上,我们选择了耐力负荷极大,处于高度疲劳状态下的越野滑雪运动员与竞跑田径运动员为试验对象,分别进行了为期6个月与1个月服药后的人体耐力效应评价。服用后,若干运动心功能指标与疲劳代谢产物有显著变化,竞赛成绩亦有明显提高,并且无不良反应。方法与结果如下:一、实验材料1 实验仪器(1)XF-5型心率发射器上海体育技术研究所(2)RDH-2000型氧与二氧化碳分析仪南京分析仪器厂(3)XY-1型动态心功能测量仪海军医学研究所(4)μ-80型微型计算机上海计算机研究所     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

天然生化耐力增强剂药效研究

<正> 随着世界医学模式的变化,预防医学日益显示出重要的作用,为了强身保建,防病治病,加速消除智力与体力的疲劳,提高机体的耐力,近年来,国内外医学界,体育界等许多研究单位颇为重视抗疲劳物质与耐力增强剂的研究。     

水鲨头皮胶原蛋白分离及其部分特性研究

目的从水鲨头皮分离得到胶原蛋白并研究其部分生化特性。方法水鲨头皮用不同浓度的氢氧化钠溶胀，去离子水清洗至中性，经胃蛋白酶限制性酶解，离心，上清液经透析后冷冻干燥，得到胶原蛋白制品。用聚丙烯酰胺凝胶电泳、差式扫描量热仪（DSC）、圆二色谱仪等研究了分离得到的胶原蛋白的特性。结果胶原蛋白的回收率为鲜重的2．9％-4．1％；在十二烷基硫酸钠聚丙烯酰胺凝胶电脉（SDS—PAGE）上呈现清晰的3条谱带，相对分子质量分别为205000、134000、118000，热变性温度为69℃。结论分离得到的胶原蛋白可进一步用于微囊剂、纳米材料等生化材料的研究。     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

鲨烯复合制剂对机体耐力的影响

鲨烯是从深海大型鲨鱼—姥鲨 (basking shark)肝脏中提取的接近无色的油状液体 ,其结构类似β-胡萝卜素异戊间二烯化合物 [1 ] 。姥鲨栖息在深海环境中 ,尽管处于低温、阴暗、缺氧等条件下 ,但它们仍具强大的生命力。研究发现 ,大型鲨鱼肝脏巨大 ,其肝脏重量约占体重的 2 5 % ,占内脏总重量的 90 % ,其肝脏含有大量的鲨烯为其活力之源 [2 ] 。本项研究采用鲨烯为主要成分 ,配以适量蜂皇浆、人参皂甙等制成复合剂 (下称鲨烯制剂 ) ,经动物游泳、爬杆、离体心脏灌流试验、动物雄性器官等观察 ,表明其具有提高机体体力与耐力等功效 ,并经过动物…     

一株产新型大环内酯抗生素海洋解淀粉芽孢杆菌的分离鉴定

Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.     

一株产类胡萝卜素海洋细菌的分离及系统分类学研究

目的 对一株分离自我国东海海域160 m深处产类胡萝卜素的海洋细菌进行形态学、生理生化特性及分子水平的系统分类学研究.方法 通过细菌形态学特征观察、生长条件、生理生化特征测定、全细胞脂肪酸成分分析、16S rRNA序列扩增、测序、比对及构建系统发育进化树,研究了该菌株的系统分类学特性;并通过高效液相色谱对该菌株的类胡萝卜素产出特性进行了分析.结果 该菌株与考克氏菌属(Kocuria sp.)相似度最高,顺-7-棕榈油酸/顺-6-棕榈油酸为该菌株的主要脂肪酸组分.结论 该菌株属于考克氏菌属,暂定名为考克氏菌CAR-red(Kocuria sp.CAR-red)株.     

异位妊娠失血性休克手术麻醉方法的探讨

我科从１９８８年１月至１９９９年１月共行宫外孕手术１３８例,采用不同麻醉方法,现总结如下。１　临床资料１１　一般资料：本组１３８例病人发病年龄２１岁～２５岁计２１例。２６岁～３０岁计５８例。３１岁～３５岁４１例,３６岁～４２岁１８例。１２　入院时间与出血量：病人发病后３０ｍｉｎ～１ｈ左右急诊入院,出血量最多达３０００ｍｌ,最少达１２００ｍｌ。１３　麻醉方法：采用局部浸润麻醉（ｌＩ）和氯胺酮（ＫＴ）静脉麻醉相结合方法８８例即（ｌＩ＋ＫＴ）。５０例采用硬膜外阻滞麻醉（ＥＢ）。二组患者年龄、体重…