社区卫生服务机构开展糖尿病同伴教育的SWOT分析

As the situation of diabetes in China is becoming serious in recent years,control in diabetes health education and management work has become the main means.Exploring the effective way of healthy education and promoting the development of community diabetes management are to realize our country grass-roots health services equalization.With health education that is as a community's healthy service institutions being an important means for management of diabetes,the single mode of traditional education which is more insufficient influences and restricts the development of community diabetes management.In China,with the lack of medical resources,peer education as a new way can fully integrate resources which the community health service institutions own.The promotion of community has significance for patients to control diseases and the prevention of complications to provide support.In recent days,staffs from health care can't cognize the significance of peer education,peer education intervention mode is simple,and there has not yet been community norms implementation model and effectiveness of the evaluation criteria,but external environment and internal conditions still exist many deficiencies that hinder the peer education in community diabetes further development.In this paper,the SWOT analysis to carry out peer education on diabetes can make full use of internal strengths and avoid the external risks from the four dimensions of the strengths,weaknesses,opportunities and threats analysis.This paper puts forward the countermeasure for diabetes of peer education in community;also provide reference for the management of community chronic disease.      

PD98059抑制大鼠听泡成骨细胞增殖及分化

目的 探究ERK抑制剂PD98059对大鼠听泡成骨细胞增殖及分化的影响。方法 用0.25%胰酶和I型胶原酶两步消化法提取SD乳鼠听泡成骨细胞,分别与浓度为0、10、25和50μmol/L的ERK抑制剂PD98059进行共培养,然后采用EDU法连续4天对4组成骨细胞的增殖进行检测,对各组增殖趋势进行对比分析。用10 mmol/L、50μg/ml和10^-7 mol/L的浓度的β-甘油磷酸钠、L-维生素C和地塞米松对4组成骨细胞进行成骨诱导分化,24 h后采用RT-qPCR技术检测各组成骨相关因子Ocn、Bsp、Runx2、Bmp2、OPG和RANKL mRNA的表达水平,分析结果。结果 浓度为10、25和50μmol/L的ERK抑制剂PD98059对SD乳鼠听泡成骨细胞的增殖均有不同程度的抑制作用,其中浓度为10μmol/L的PD98059对其增殖的抑制作用明显大于其余3组(P<0.05)。此外,上述不同浓度的PD98059对Ocn、Bsp、Runx2、Bmp2和OPG mRNA的表达均有不同程度的抑制作用,其中Ocn、Bsp、Runx2和Bmp2 mRNA的表...     

两种不同方法治疗儿童腺样体肥大的临床对照研究

目的：比较药物治疗和手术切除在腺样体肥大儿童中的临床效果,指导临床治疗。方法：选择Ⅱ级及以上腺样体肥大的60例患儿分成观察组和对照组各30例,观察组给予药物治疗,对照组给予手术切除,干预前及干预后3个月后对两组患儿采用肺功能、OSA-18评分表及过敏症状调查表进行评估。结果：两组患儿疗效相当,但观察组患儿身体不适症状明显好转,评分为（9.25±3.26）分,而对照组为（14.82±3.32）分;观察组过敏症状明显减轻,总有效率83.3%,而对照组仅66.7%;观察组肺功能改善更明显,总有效率90.0%,而对照组为76.6%,差异有统计学意义（P<0.05）。结论：腺样体肥大Ⅱ级及以上患儿药物治疗更适合,尤其是6岁以下患儿,疗效不佳时经OSA-18评分证实严重影响生活质量的患儿可考虑手术切除腺样体及扁桃体。     

大鼠听泡原代成骨细胞分离培养及鉴定

目的 对SD大鼠听泡的原代成骨细胞进行分离培养及鉴定。方法 选取6只出生24 h的SD乳鼠，从颞区无菌分离出听泡组织，经0.25%胰蛋白酶和I型胶原酶消化后接种培养；待生长融合至80%密度时，进行传代培养，于倒置显微镜下观察原代及传代成骨细胞的生长状态，并用EDU法分析其增殖趋势。对生长良好的细胞进行成骨诱导分化，并用ALP试剂盒和茜素红试剂盒进行染色分析，鉴定分化效果。结果 大鼠听泡原代及传代成骨细胞培养中，细胞均贴壁良好、生长旺盛，第2 d分别可融合生长至50%、30%的密度，第4 d分别可融合生长至80%、70%的密度，形态呈现多样化。EDU法检测分析见：第1～4 d细胞增殖比均维持在较高水平，从第5 d起呈直线下降趋势。经诱导分化后，细胞生长状态良好，形态依旧多样化，早期以梭形为主，中后期形态较为丰腴。分化后2周，ALP染色见大量细胞胞质呈现深蓝色；第15 d经茜素红染色便可见局部出现橙红色钙化结节。结论 通过胰酶联合胶原酶两步消化法，成功地从SD乳鼠听泡中分离出原代成骨细胞，所得细胞生长及传代能力强，经诱导分化后具备良好的成骨活性，为鼓室硬化等疾病的体外研究提供可能。