去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.     

TAFI与PAI-1及t-PA水平与系统性红斑狼疮患者下肢深静脉血栓形成的关系

目的探讨系统性红斑狼疮(SLE)患者血浆凝血酶激活纤溶抑制物(TAFI)、纤溶酶原激活物抑制剂(PAI-1)及组织型纤溶酶原激活物(t-PA)水平, 以及其与下肢深静脉血栓形成的关系。方法本研究采用病例对照研究方案, 回顾性选取山东省聊城市人民医院2018年6月至2021年6月收治的发生下肢深静脉血栓的SLE患者32例(血栓组), 其中男4例, 女28例, 年龄(49.7±5.5)岁;选取同期未发生下肢深静脉血栓的SLE患者64例(对照组), 其中男11例, 女53例, 年龄(50.8±5.5)岁。比较两组患者血浆TAFI、PAI-1及t-PA水平, 多因素分析采用logistic回归模型, 分析其与SLE患者下肢深静脉血栓形成的关系。结果血栓组SLE患者血浆TAFI、PAI-1、t-PA水平分别为(32.77±5.17)mg/L、(29.43±5.51)μg/L、(6.58±1.40)μg/L, 对照组分别为(23.56±4.40)mg/L、(19.00±4.40)μg/L、(9.40±2.23)μg/L, 血栓组TAFI、PAI-1水平高于对照组, t-PA水平低于对照组(均P&l...     

去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.     

去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.     

健康教育对鼻内镜手术后患者自我护理能力的影响

鼻内镜手术是治疗鼻外科疾病的重要手段,由于目前术后鼻腔局部处理是长期、繁琐的过程,有时需持续半年甚至更长时间,因此,加强自我护理能力,防止各种并发症和残障,促进患者早日全面康复至关重要。由于患者出院后大多缺乏鼻内镜手术后护理的有关知识,故如何加强此类患者的健康教育,增强自     

光固化流动树脂粘接正畸托槽的临床观察

目的 观察Esthet-Ⅹ Flow光固化流动树脂粘接正畸托槽的临床效果.方法 选取接受正畸矫治的患者50例,随机分为实验组和对照组.实验组采用光固化流动树脂粘接托槽,对照组采用京津牙釉质粘合树脂粘接托槽.比较两组患者3个月及6个月时托槽总脱落率及6个月时前磨牙托槽的脱落率.结果 实验组3个月和6个月时的托槽总脱落率均低于对照组（P＜0.05）.实验组6个月时前磨牙托槽脱落率低于对照组（P＜0.05）.结论 光固化流动树脂粘接托槽的临床效果优于京津牙釉质粘合树脂.     

铁碳纳米粒介导顺铂对喉癌细胞敏感性的研究*

背景：应用纳米技术对化疗药物进行靶向性改进是增加肿瘤对化疗药物敏感性的有效手段之一。 目的：观察铁碳纳米粒搭载顺铂对喉癌Hep-2细胞的抑制作用及对细胞Caspase 3,Survivin mRNA表达的影响。 方法：分别应用铁碳纳米粒和(或)顺铂的生理盐水分散液干预喉癌Hep-2细胞,以生理盐水干预的细胞作为对照。 结果与结论：MTT检测结果显示,顺铂可明显抑制Hep-2细胞的生长,与铁碳纳米粒联合应用对Hep-2细胞的抑制作用更强;表现为细胞生长不贴壁,增殖缓慢,出现凋亡征象。RT-PCR结果显示,共培养5 d,铁碳纳米粒和顺铂联合及顺铂单独处理的Hep-2细胞Caspase 3 mRNA水平明显增高,而Survivin mRNA水平明显降低,以铁碳纳米粒和顺铂联合作用效果更明显,而单独应用铁碳纳米粒对Hep-2细胞无影响。提示铁碳纳米粒搭载顺铂能够增加喉癌Hep-2细胞对顺铂的敏感性,提高药物化疗效果。 关键词：铁碳纳米粒;顺铂;喉癌;Hep-2细胞;化疗;敏感性;Caspase3;Survivin doi:10.3969/j.issn.1673-8225.2012.12.010     

伴有多种FISH异常多发性骨髓瘤患者的预后分析

目的分析同时伴有2种及2种以上的荧光原位杂交(FISH)结果异常的多发性骨髓瘤(MM)患者的预后情况,筛选预后较差的MM者。方法共计纳入2011年7月至2017年2月期间767例在长征医院就诊的初诊MM患者,所有患者初诊时均进行FISH检测,所检测的FISH异常包括IgH易位、t(4;14)、t(11;14)、t(14;16)、17p-、1q扩增、-13/13q-,分析所有FISH异常对预后的影响。结果多因素COX回归分析结果示1q扩增、17p-、t(4;14)是MM患者的独立不良预后因素,进一步将1q扩增、17p-、t(4;14)3种预后因素按不同组合将患者分为7组,仅伴有1q扩增患者295例(38%),仅伴有17p-患者37例(4.8%),仅伴有t(4;14)的患者38例(4.9%),同时伴有1q扩增和17p-的患者42例(5.4%),同时伴有1q扩增和t(4;14)的患者77例(10.0%),同时伴有t(4;14)和17p-的患者为6例(0.7%),同时伴有1q扩增、t(4;14)和17p-3种FISH异常的患者为13例(1.7%)。因最后2种情况患者例数较少,未纳入生存分析。前5组MM患者的3年无进展生存(PFS)率分别为:32.6%、27.0%、60.4%、27.3%、25.7%,3年总生存(OS)率分别为:63.0%、52.8%、86%、59.0%、55.2%。仅伴有1q扩增或仅伴有17p-的患者和同时伴有1q扩增及17p-患者比较,其OS差异无统计学意义(P=0.651,P=0.339)。结论 17p-和1q扩增为MM的高危预后因素,但同时伴有17p-和1q扩增的患者相较于仅单独伴有17p-或1q扩增的患者其不良预后风险并不增加。     

去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.