云南省中医药民族民间经验方、经典名方的开发思路

云南中医民族药源远流长,造就了大量民族民间经验方和经典名方,在临床医疗和相关科研工作中有不可或缺的作用。通过对云南省的民族民间经验方、经典名方的整理情况,发展现状及存在的问题,二次开发研究情况分析,得到云南中医药民族民间经验方、经典名方启示开发思路。制约民族民间经验方、经典名方的主要问题为:资料收集整理不齐,临床应用缺乏相应的质量考评标准和系统的安全性总结;现行法律法规对民族民间经验方、经典方有效性及合法产权界定尚不完善;二次开发利用不创新等。对流落民间,口耳相传但尚未被整理在册的药方应该及时保存记录,妥善保护,建立安全性,有效性评价标准;建立云南民族医药数据库,完善民族医药保护制度;对现有民族民间经验方、经典名方进行创新及二次开发。     

云南省医疗机构制剂质量标准存在的问题及思考

为医疗机构制剂标准提高工作提供参考。收集整理2018年云南省医疗机构制剂标准整顿工作中存在的问题,分析其主要原因并提出建议。发现部分医疗机构制剂质量标准陈旧,存在处方工艺不规范、检验项目较为落后等问题,需要医疗机构提高认识、整合资源、科学管理持续研究,监管部门多方位把控和帮促,共同建立医疗机构质量标准持续提升机制,保证制剂质量与安全。     

壳聚糖与海藻酸钙双相陶瓷骨支架的机械性能及细胞相容性

BACKGROUND: Hydroxyapatite/β-tricalcium phosphate biphasic ceramic bone has good cell compatibility, but its mechanical properties are poor. OBJECTIVE: To construct chitosan/ or calcium alginate/biphasic ceramic bone scaffolds and to detect their mechanical properties and cytocompatibility. METHODS: Different concentrations of chitosan (2%, 4%, 7%, 10%) or calcium alginate (3%, 4%, 5%, 7%) were mixed with biphasic ceramic bone to prepare chitosan/biphasic ceramic bone scaffold and calcium alginate/biphasic ceramic bone scaffold. Their morphology and structure, coagulation time, anti-dissolution properties, shear force, compressive strength and cell compatibility were detected. RESULTS AND CONCLUSION: (1) Coagulation time: with the concentration increase, the initial and final setting time of these two kinds of composite scaffolds were prolonged to some extent. (2) Scanning electron microscopy: these two kinds of composite scaffolds showed porous microstructures with different pore sizes. (3) Anti-dissolution properties: the calcium alginate/biphasic ceramic bone scaffold (3%, 4%, 5%, 7%) and chitosan/biphasic ceramic bone scaffold (7%, 10%) had good anti-dissolution properties in the liquid. (4) Mechanical strength: with the concentration increase, the shear force and compressive strength of the calcium alginate/biphasic ceramic bone scaffold were reduced. (5) Cell compatibility: the cytotoxicity of chitosan/ or calcium alginate/biphasic ceramic bone scaffolds was graded as 0-1 or 2-3, respectively. These results show that the chitosan/biphasic ceramic bone scaffold has better mechanical properties and cell compatibility than the calcium alginate/biphasic ceramic bone scaffold.      

混合信号诱导的大鼠骨髓间充质干细胞与支架材料复合成牙的体内研究

目的:研究经碱性成纤维细胞生长因子(bFGF)与骨形态发生蛋白2 (BMP-2)混合信号诱导的大鼠骨髓间充质干细胞(BMSCs)在体内环境中与不同支架材料复合后的成牙潜能。方法:将取自SD大鼠的BMSCs与牙胚细胞在bFGF和BMP-2混合信号诱导的微环境中共培养后分别接种于明胶海绵和3D打印的PVA/DCCP复合支架材料上,然后回植入同种异体大鼠肾被膜下。在回植后的第1、3、7、14、28天采集回植体样本,用实时定量PCR (RT-PCR) 检测各组样本成牙相关基因成釉蛋白 (AMBN)、牙本质基质蛋白1 (DMP1)、Ⅰ型胶原蛋白 (Collagen-Ⅰ)、以及同源异型盒基因1 (DLX1) mRNA水平。结果:各基因在不同时间点的表达量与完整牙胚(E组)均存在统计学差异(P＜0.05);上述4个成牙相关基因的表达水平在不同支架材料组之间具有显著性差异(P＜0.001);不同成牙诱导信号对各个基因的表达水平也有一定影响。结论:在体内模拟环境下,经bFGF与BMP-2混合信号诱导的大鼠BMSCs与3D打印支架材料复合后能提高成牙基因的表达水平,促进牙体组织的形成。优选支架材料并构建合适的组织工程牙模型对实现非牙胚源性细胞向牙源性细胞的转化同样具有重要意义     

生物功能性义齿修复牙列缺失的临床效果评价

目的 评价生物功能性义齿修复的临床效果,为无牙牙合患者优化诊疗方案提供科学依据,提高全口义齿修复效果。方法 选取2019年3月至2020年3月就诊于乌鲁木齐市口腔医院样本20例,依据修复方式的不同,分为BPS试验组和对照组,每组各10例,分别行生物功能性修复义齿（BPS试验组）和传统修复义齿（对照组）,检测戴牙后即刻、2、6、9个月时义齿的固位力、咀嚼效率及调磨的压痛点数、舒适视觉模拟量表（VAS）数值,进行比较。结果 戴牙后即刻、2、6、9个月,试验组固位力和咀嚼效率优于对照组,特别是下颌的固位力有明显优势,差异有统计学意义（P<0.5）;在戴牙后2个月内,BPS试验组调磨的压痛点数少于对照组,差异有统计学意义（P<0.5）;在戴牙后即刻及2个月内,BPS试验组VAS评分高于对照组,差异有统计学意义（P<0.5）。结论 生物功能性义齿在全口义齿修复中有较好的诊疗效果,在严格控制修复过程的前提下,适宜临床推广。     

实施中药材 GAP 与中药生产调研报告

目的：了解中药材生产与中药制剂生产的情况,促使《中药材生产质量管理规范》(GAP)和《药品生产质量管理规范》(GMP)相互结合,进一步推动中药材 GAP 实施。方法采取现场走访、与企业座谈及发放调查问卷相结合的方式进行调研,根据调研情况和对问卷的统计分析得出调研结果。结果与结论中药材 GAP 自发布以来已经实施十多年,但在 GAP 认证体系建设、基地建设与政策扶持、推进中药材GAP 进程方面仍有待深入总结研究。调研显示,种植和使用 GAP 药材成本提高导致价格竞争力减弱,挫伤了企业实施中药材 GAP 的积极性;公司与农户合作种植的模式存在农户积极性降低、对农户制约不严、产品溯源性差等弊端;缺乏有效的中药材价格调控机制;GAP 药材的种植管理缺乏一致性。建议参照新修订药品 GMP 的评定方式,对中药材 GAP 种植企业实施可持续性地改进。     

云南省药用香料植物发展现状及对策建议

云南省作为公认的天然香料植物大省,拥有着得天独厚的香料植物资源。而药用香料植物的开发利用少之又少,因此,本研究对云南省药用香料植物的资源状况、发展现状和开发中存在的问题进行阐述,为其资源综合开发利用提供一定的对策建议。     

藏药珊瑚通窍丸的质量控制方法探讨

目的:在藏药珊瑚通窍丸的原有质量标准基础上进行提高性研究,以期增强了本品质量的可控性.方法:采用高效液相色谱法对羟基红花黄色素A进行了含量测定研究,并对其它有利于珊瑚通窍丸质量控制水平提高的指标进行初步探索.结果:高效液相色谱法能满足羟基红花黄色素A的测定要求,依据药典现有方法或通则进行的其它指标的测定也都能得出有指导...     

圆锥型套筒冠义齿和传统可摘义齿修复孤立基牙的三维有限元分析

BACKGROUND: The conical telescopic crown denture has a better long-term effect on dentition defects of few remaining teeth than the traditional removable denture. However, there are few studies to analyze the application of these two kinds of repair methods using the finite element method. OBJECTIVE: To compare the stress distribution of the isolated abutment tooth repaired using the conical telescopic crown denture and traditional removable denture. METHODS: Two-dimensional images of the mandible and dentition in healthy adults were scanned by CT. Three-dimensional finite element models of conical telescopic crown denture and traditional removable denture were established in the mandible only with the bilateral canines. Under the same vertical and oblique loadings, the maximum equivalent stress of the abutment, periodontal membrane or edentulous alveolar ridge was compared between two repair methods. RESULTS AND CONCLUSION: Under the vertical and oblique loadings, the total stress of abutment and periodontal membrane in the traditional removable denture was higher than that in the conical telescopic crown denture and the stress in the corresponding ring was concentrated obviously; the total stress of edentulous alveolar ridge in the conical telescopic crown denture was slightly larger than that in the traditional removable denture, but the stress was well-distributed and concentrated in a small area. Under the oblique loading, the total stress of abutment, periodontal membrane and edentulous alveolar ridge was higher than that under the vertical loading in these two dentures, and the stress was concentrated in the periodontal membrane of lingual neck, but the total stress in the traditional removable denture was larger, with a wider concentration range. These findings indicate that the stress on the supporting tissue of conical telescopic crown denture is more evenly distributed within a smaller range than that of traditional removable denture, and the former is more fit for the health of abutment, periodontal tissues and edentulous alveolar ridge.      

bFGF/BMP-2转染大鼠骨髓干细胞体内成牙的研究

目的: 牙胚细胞和成纤维生长因子(Fibroblast growth factor bFGF)/骨形成蛋白2(bone morphogenetic protein 2 BMP-2)联合转染的骨髓间充质干细胞 (bone marrow stem cells,BMSCs)混合培养,复合明胶海绵支架构建牙组织工程植入大鼠体内,探究其成牙能力。方法: 取健康SD大鼠128只,随机分为4组:细胞团块组;明胶海绵组;细胞团块+明胶海绵组;空白对照组,不做任何干预,均在全麻下植入大鼠肾被膜下。术后按4个时间点(5、10、14、28 d各8只)分期取材,进行大体组织观察,Masson染色和免疫组化染色。结果: 大体组织和Masson染色:术后5、10、14、28 d形态学观察显示细胞团块+明胶海绵组形成牙样组织。免疫组化:析因分析显示细胞团块+明胶海绵组在5、10、14 d DMP-1、BMP-4、DSP表达量最高且均高于其余各组,随着时间推移表达量逐渐有减小趋势,差异有统计学意义(P＜0.05)。结论: 牙胚细胞和基因转染BMSCs混合培养,复合明胶海绵构建牙组织工程的体内实验结果形成牙样组织,为组织工程牙的成功构建奠定了一定的基础。