全文获取类型
收费全文 | 13547篇 |
免费 | 1289篇 |
国内免费 | 25篇 |
专业分类
耳鼻咽喉 | 160篇 |
儿科学 | 428篇 |
妇产科学 | 648篇 |
基础医学 | 2156篇 |
口腔科学 | 259篇 |
临床医学 | 1827篇 |
内科学 | 2316篇 |
皮肤病学 | 239篇 |
神经病学 | 1189篇 |
特种医学 | 430篇 |
外科学 | 1239篇 |
综合类 | 294篇 |
一般理论 | 33篇 |
预防医学 | 1492篇 |
眼科学 | 294篇 |
药学 | 847篇 |
中国医学 | 10篇 |
肿瘤学 | 1000篇 |
出版年
2023年 | 82篇 |
2022年 | 136篇 |
2021年 | 243篇 |
2020年 | 230篇 |
2019年 | 255篇 |
2018年 | 297篇 |
2017年 | 235篇 |
2016年 | 265篇 |
2015年 | 272篇 |
2014年 | 427篇 |
2013年 | 605篇 |
2012年 | 830篇 |
2011年 | 882篇 |
2010年 | 457篇 |
2009年 | 422篇 |
2008年 | 760篇 |
2007年 | 739篇 |
2006年 | 715篇 |
2005年 | 711篇 |
2004年 | 695篇 |
2003年 | 589篇 |
2002年 | 562篇 |
2001年 | 297篇 |
2000年 | 298篇 |
1999年 | 257篇 |
1998年 | 151篇 |
1997年 | 125篇 |
1996年 | 113篇 |
1995年 | 126篇 |
1994年 | 129篇 |
1993年 | 108篇 |
1992年 | 209篇 |
1991年 | 172篇 |
1990年 | 195篇 |
1989年 | 187篇 |
1988年 | 176篇 |
1987年 | 152篇 |
1986年 | 127篇 |
1985年 | 120篇 |
1984年 | 127篇 |
1983年 | 97篇 |
1982年 | 81篇 |
1980年 | 80篇 |
1979年 | 124篇 |
1978年 | 100篇 |
1977年 | 74篇 |
1976年 | 76篇 |
1974年 | 92篇 |
1973年 | 70篇 |
1972年 | 75篇 |
排序方式: 共有10000条查询结果,搜索用时 156 毫秒
1.
Christopher D Hue Frances S Cho Siqi Cao Cameron R ”Dale” Bass David F Meaney Barclay Morrison III 《Journal of cerebral blood flow and metabolism》2015,35(7):1191-1198
Owing to the frequent incidence of blast-induced traumatic brain injury (bTBI) in recent military conflicts, there is an urgent need to develop effective therapies for bTBI-related pathologies. Blood-brain barrier (BBB) breakdown has been reported to occur after primary blast exposure, making restoration of BBB function and integrity a promising therapeutic target. We tested the hypothesis that treatment with dexamethasone (DEX) after primary blast injury potentiates recovery of an in vitro BBB model consisting of mouse brain endothelial cells (bEnd.3). DEX treatment resulted in complete recovery of transendothelial electrical resistance and hydraulic conductivity 1 day after injury, compared with 3 days for vehicle-treated injured cultures. Administration of RU486 (mifepristone) inhibited effects of DEX, confirming that barrier restoration was mediated by glucocorticoid receptor signaling. Potentiated recovery with DEX treatment was accompanied by stronger zonula occludens (ZO)-1 tight junction immunostaining and expression, suggesting that increased ZO-1 expression was a structural correlate to BBB recovery after blast. Interestingly, augmented ZO-1 protein expression was associated with specific upregulation of the α+ isoform but not the α− isoform. This is the first study to provide a mechanistic basis for potentiated functional recovery of an in vitro BBB model because of glucocorticoid treatment after primary blast injury. 相似文献
2.
3.
Understanding the contribution of endothelial cells to the progenitor pools of adult tissues has the potential to inform therapies for human disease.To address whether endothelial cells transdifferentiate into non-vascular cell types,we performed cell lineage tracing analysis using transgenic mice engineered to express a fluorescent marker following activation by tamoxifen in vascular endothelial cadherin promoter-expressing cells(VEcad-CreERT2;B6 Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze).Activation of target-cell labeling following 1.5 months of ad libitum feeding with tamoxifen-laden chow in 4–5 month-old mice resulted in the tracing of central nervous system and peripheral cells that include:cerebellar granule neurons,ependymal cells,skeletal myocytes,pancreatic beta cells,pancreatic acinar cells,tubular cells in the renal cortex,duodenal crypt cells,ileal crypt cells,and hair follicle stem cells.As Nestin expression has been reported in a subset of endothelial cells,Nes-CreERT2 mice were also utilized in these conditions.The tracing of cells in adult Nes-CreERT2 mice revealed the labeling of canonical progeny cell types such as hippocampal and olfactory granule neurons as well as ependymal cells.Interestingly,Nestin tracing also labeled skeletal myocytes,ileal crypt cells,and sparsely marked cerebellar granule neurons.Our findings provide support for endothelial cells as active contributors to adult tissue progenitor pools.This information could be of particular significance for the intravenous delivery of therapeutics to downstream endothelial-derived cellular targets.The animal experiments were approved by the Boise State University Institute Animal Care and Use Committee(approval No.006-AC15-018)on October 31,2018. 相似文献
4.
5.
6.
7.
Annals of Surgical Oncology - 相似文献
8.
9.
Béla Nagy Zsolt Bene Zsolt Fejes Sonya L. Heltshe David Reid Nicola J. Ronan Yvonne McCarthy Daniel Smith Attila Nagy Elizabeth Joseloff György Balla János Kappelmayer Milan Macek Scott C. Bell Barry J. Plant Margarida D. Amaral István Balogh 《Journal of cystic fibrosis》2019,18(2):271-277
Background
We have recently shown that human epididymis protein 4 (HE4) levels correlate with the severity of cystic fibrosis (CF) lung disease. However, there are no data on how HE4 levels alter in patients receiving CFTR modulating therapy.Methods
In this retrospective clinical study, 3 independent CF patient cohorts (US-American: 29, Australian: 12 and Irish: 19 cases) were enrolled carrying at least one Class III CFTR CF-causing mutation (p.Gly551Asp) and being treated with CFTR potentiator ivacaftor. Plasma HE4 was measured by immunoassay before treatment (baseline) and 1–6?months after commencement of ivacaftor, and were correlated with FEV1 (% predicted), sweat chloride, C-reactive protein (CRP) and body mass index (BMI).Results
After 1?month of therapy, HE4 levels were significantly lower than at baseline and remained decreased up to 6?months. A significant inverse correlation between absolute and delta values of HE4 and FEV1 (r?=??0.5376; P?<?.001 and r?=??0.3285; P?<?.001), was retrospectively observed in pooled groups, including an independent association of HE4 with FEV1 by multiple regression analysis (β?=??0.57, P?=?.019). Substantial area under the receiver operating characteristic curve (ROC-AUC) value was determined for HE4 when 7% mean change of FEV1 (0.722 [95% CI 0.581–0.863]; P?=?.029) were used as classifier, especially in the first 2?months of treatment (0.806 [95% CI 0.665–0.947]; P?<?.001).Conclusions
This study shows that plasma HE4 levels inversely correlate with lung function improvement in CF patients receiving ivacaftor. Overall, this potential biomarker may be of value for routine clinical and laboratory follow-up of CFTR modulating therapy. 相似文献10.