老年黄斑变性的视网膜色素上皮及其视功能的改变

目的：应用黄斑区密集点矩阵视野并结合眼底荧光血管造影电脑眼底图像分析老年黄斑变性视网膜色素上皮损害与ＤＭＭＭ的视野光敏感度的改变，找出改变的规律有评价其应用价值。方法：用Ｈｕｍｐｈｒｅｙ－６４０视野计自行设计ＤＭＭＭ检测ＡＭＤ干性组５１人６４只眼湿性且２３人２７只眼，用Ｚｅｉｓｓ公司生产的ＫＯＮＴＲＯＮＥＬＥＫＴＲＯＮＩＫ Ｉｎａｇｅ Ａｎａｌｙｓｉｓ Ｄｉｖｉｓｉｏｎ－计算机图像分析仪总丢失量显     

老年抑郁障碍患者的心理干预对比分析

目的 :探讨老年抑郁障碍心理干预的效果。方法 :采用对比研究的方法 ,在流行病学调查的基础上选择符合CCMD -Ⅱ -R诊断标准的 72例抑郁性神经症和 10例抑郁症患者 ,按性别、年龄、文化等基本相同 ,分成对照和实验组各一半 ,实验组给以 6周的心理干预 ,两组均不用药物治疗 ,分别于治疗前及三个月后 ,测评老年抑郁量表、生活满意度指数及社会支持评定量表 ,并进行对比分析。结果 :心理干预前两组老年抑郁量表 (SDS)得分分别为 17 41± 3 19、 17 5 1± 2 44,三个月后对照组下降不显著 ,而实验组明显下降 ( 17 5 1± 2 44/ 8 93± 5 5 2 ,t=10 89,P <0 0 0 1) ;生活满意度 (t=4 33,P <0 0 0 1)实验组显著提高 ;对照组无明显变化 ,社会支持评定实验组 ( 31 15± 6 37/ 36 32± 6 86 ,t =4 93,P <0 0 0 1)也显著提高 ,而对照组则不明显。结论 :社区综合性心理干预可改变抑郁性神经症患者的抑郁情绪 ,改善认知水平 ,从而提高生活满意度。     

Prevalence and factors associated with late diagnosis among older adults living with HIV in liuzhou,China: 2010−2020

This paper aimed to quantify and characterize the prevalence and associated factors for late diagnosis in older adults living with human immunodeficiency virus (HIV) in Liuzhou, China, from 2010 to 2020. The characteristics of older adults living with HIV were described separately in time, space and population. Multivariate logistic regression analysis evaluates the factors influencing late diagnosis in HIV-positive adults ≥ 50 years of age. The majority of older adults living with HIV were over 60 years old, male, and with CD4 counts < 200 cells/μl at diagnosis, with most late diagnoses being more likely to report heterosexual transmission. These two factors may potentially provide a positive influence on late diagnosis: older and CD4 counts < 500 cells/μl. In contrast, females and those with homosexual or other transmission provide a negative. These results suggest that late diagnosis of HIV-positive adults ≥ 50 years of age remains a severe and growing epidemiological issue.     

新生血管性青光眼视乳头筛板结构的研究

采用正常死者眼球和新生血管性青光眼的筛板组织, 研究高眼压对视乳头筛板和结缔组织的作用． 正常眼球１２ 只, 新生血管性青光眼眼球１２ 只, 摘除眼球后用１０ ％ 福尔马林溶液固定, 切取视乳头组织约２ ｍ ｍ ×２ ｍｍ , 石蜡包埋, 苏木素－ 伊红染色, 免疫细胞化学 Ａ Ｂ Ｃ 法染色． 结果： 正常人筛板的横纵切面具有特定形态, 新生血管性青光眼筛板孔板变形, 筛孔周围结缔组织断裂, 丧失了筛板结构． 结果表明：高眼压下筛孔板和结缔组织的改变, 造成对筛板孔神经纤维的挤压, 阻滞了神经纤维轴浆的运输, 导致神经纤维功能上的损害和相应的视野缺损．     

B超在近视眼巩膜后葡萄肿诊断中的应用价值

目的 :探讨B超在近视眼巩膜后葡萄肿中的应用价值。方法 :用B型超声仪检查 >3.0 0D的 38例76眼进展性近视眼的巩膜后葡萄肿的发生情况。结果 :76眼中 5 8眼发生后葡萄肿。其中各级后葡萄肿的平均屈光度及眼轴差异均有非常显著性意义 (P <0 .0 1和P <0 .0 0 1)。 1～ 4级后葡萄肿与 0级葡萄肿的弧形斑发生率比较差异有非常显著性意义 (P <0 .0 1)。结论 :B超是诊断巩膜后葡萄肿最安全、快速、可靠的方法 ,对近视眼的预后判断有着重要作用。近视眼的屈光度和眼轴随巩膜后葡萄肿的加重而增加 ,眼底弧形斑的形成与后葡萄肿的形成有密切关系     

HPLC法测定冠心通络片中橙皮苷的含量

目的:建立冠心通络片中橙皮苷含量的测定方法。方法:采用高效液相色谱法,色谱柱为HypersilODSC18(5μm,4.6mm×100mm);流动相为甲醇-5%醋酸溶液(30∶70);检测波长为283nm;柱温为30℃;流速为1.0mL/min。结果:橙皮苷在419.2～2096μg(r=0.9998,n=5)范围内呈良好的线性关系;平均回收率98.71%;RSD为1.13%。结论:本法检测快速,定量准确,可用于冠心通络片的定量分析。     

Ortho-substituted PCBs kill thymocytes.

The effects of exposure of acutely dissociated rat thymocytes to various polychlorinated biphenyl (PCB) congeners were examined using flow cytometry. Non-planar, ortho-substituted congeners caused a rapid cell death at low micromolar concentrations, while coplanar, dioxin-like congeners at the same concentration were without significant effect. The most potent of the congeners studied was PCB 52 (2,2',5,5'-tetrachlorobiphenyl), which had an IC50 of 3.96 microM at 20 min. Prior to loss of viability there was a decrease in mitochondrial membrane potential Delta Psi m, an accumulation of intracellular calcium, and a progressive leakiness of the plasma membrane. Application of PCB 52 in calcium-free medium reduced the calcium accumulation, but did not reduce cell death. Agents that depolarized mitochondria also did not induce the same degree of cell death caused by PCB 52. Cyclosporin A, which prevents opening of the mitochondria permeability transition channel, protected against cell death but did not protect against mitochondrial depolarization or calcium accumulation. Rapamycin and FK 506 at high concentration provided partial protection against cell death. These observations indicate that the ortho-substituted PCB 52 disrupts plasma, mitochondrial and endoplasmic reticulum membranes. We hypothesize that PCB 52 incorporates into lipid bilayers and with its bulky, three-dimensional ortho-substituted congener structure disrupts membrane function to a greater degree than coplanar congeners.     

缬沙坦对心力衰竭患者血浆细胞因子浓度的影响

目的 :探讨重度充血性心力衰竭 (CHF)患者血浆中细胞因子浓度的变化及其对缬沙坦干预的反应。方法 :选择CHF患者 80例 (CHF组 )和健康体检者 4 0例 (对照组 ) ,采用放免法测定血浆肿瘤坏死因子 (TNF α)、白细胞介素 1(IL 1)和白细胞介素 6 (IL 6 )的浓度 ;然后将CHF组随机分为常规治疗组和加用缬沙坦组各 4 0例 ,1个月后再测定上述指标。结果 :CHF组 3种细胞因子浓度均高于对照组 (P <0 .0 1) ;CHF越重 ,细胞因子的浓度越高 (P <0 .0 1) ;不同病种所致同级CHF的细胞因子浓度无明显差别 (P >0 .0 5 ) ;缬沙坦能改善心功能 ,降低上述细胞因子的浓度 (P <0 .0 1)。结论 :细胞因子在重度CHF的发展中起重要作用 ,而且与基础病无关 ;缬沙坦在改善心功能的同时 ,也降低细胞因子的血浆浓度。     

电压门控氯通道生物学功能与疾病的关系

电压门控氯通道是表达于细胞膜或细胞器质膜上的一类阴离子跨膜通道蛋白,具有调节细胞容积、参与细胞迁移、增殖与凋亡以及氧化应激等生物学功能,本综述总结近年来该通道生物学功能及其与疾病的相关研究进展。     

Modulation of cyclins and p53 in mesangial cell proliferation and apoptosis during Habu nephritis

Background

Mesangial cell (MC) proliferation and apoptosis are the main pathological changes observed in mesangial proliferative nephritis. In this study, we explored the role of cyclins and p53 in modulating MC proliferation and apoptosis in a mouse model of Habu nephritis.

Methods

The Habu nephritis group was prepared by injection of Habu toxin. Mesangiolysis and mesangial expansion were determined by periodic acid-Schiff (PAS) reagent staining. Immunohistochemical analysis of PCNA and KI67, and TUNEL staining were used to detect cell proliferation and apoptosis, respectively. Expression levels of cyclins and p53 were examined by Western blotting.

Results

PAS staining showed that mesangial dissolution appeared on days 1 and 3, and mesangial proliferation with extracellular matrix accumulation was apparent by days 7 and 14. Both PCNA and KI67 immunohistochemical analysis showed that MC proliferation began on day 3, peaked on day 3 and 7, and recovered by day 14. TUNEL staining results showed that MC apoptosis began to increase on day 1, continued to rise on day 7, and peaked on day 14. Western blot analysis showed that cyclin D1 was upregulated on day 1, cyclins A2 and E were upregulated on days 3 and 7, and p53 was upregulated on days 3, 7 and 14. There was no change in the expression levels of Bax or p21.

Conclusion

We explored the tendency for MC proliferation and apoptosis during the process of Habu nephritis and found that cyclins and p53 may modulate the disease pathology. This will help us determine the molecular pathogenesis of MC proliferation and provide new targets for disease intervention.