A Novel Variant of the Human Blood Group K1 Antigen

A discrepancy in duplicate anti-K1 typing in a parentage case led to the discovery of an unusual K1 blood group antigen. Red blood cells from the propositus (JC) express a rare variant of the K1 antigen that is detectable by only 8 of 72 sera containing anti-K1. Absorption and elution studies using reactive anti-K1 confirmed the presence of a K1 antigen. Nonreactive anti-K1 was not absorbed by or eluted from JC's red blood cells. Red cells from 3 of the propositus's siblings also had the variant K1 antigen. The variant antigen exhibited qualitative as well as quantitative differences as compared to normal K1, and we have named it K1var.     

Etiology-Based Classification of Adjacent Segment Disease Following Lumbar Spine Fusion

Background

Adjacent segment disease (ASDz) is a potential complication following lumbar spinal fusion. A common nomenclature based on etiology and ASDz type does not exist and is needed to assist with clinical prognostication, decision making, and management.

Questions/Purposes

The objective of this study was to develop an etiology-based classification system for ASDz following lumbar fusion.

Methods

We conducted a retrospective chart review of 65 consecutive patients who had undergone both a lumbar fusion performed by a single surgeon and a subsequent procedure for ASDz. We established an etiology-based classification system for lumbar ASDz with the following six categories: “degenerative” (degenerative disc disease or spondylosis), “neurologic” (disc herniation, stenosis), “instability” (spondylolisthesis, rotatory subluxation), “deformity” (scoliosis, kyphosis), “complex” (fracture, infection), or “combined.” Based on this scheme, we determined the rate of ASDz in each etiologic category.

Results

Of the 65 patients, 27 (41.5%) underwent surgery for neurogenic claudication or radiculopathy for adjacent-level stenosis or disc herniation and were classified as “neurologic.” Ten patients (15.4%) had progressive degenerative disc pathology at the adjacent level and were classified as “degenerative.” Ten patients (15.4%) had spondylolisthesis or instability and were classified as “instability,” and three patients (4.6%) required revision surgery for adjacent-level kyphosis or scoliosis and were classified as “deformity.” Fifteen patients (23.1%) had multiple diagnoses that included a combination of categories and were classified as “combined.”

Conclusion

This is the first study to propose an etiology-based classification scheme of ASDz following lumbar spine fusion. This simple classification system may allow for the grouping and standardization of patients with similar pathologies and thus for more specific pre-operative diagnoses, personalized treatments, and improved outcome analyses.

     

Competition among leukemic cells

We investigated competition among leukemic cells induced by Moloney murine leukemia virus (MoLV) in order to understand the mechanisms involved in the generation of leukemia. We used six leukemic cells lines from Balb/C mice infected with MoLV. Each line had a unique genetic marker which enabled us to trace it in mixtures of cells either in vivo or in vitro. The markers were a unique rearrangement of T-cell receptors, the integration sites of the retrovirus and rearrangements in the Pim-1 oncogene. A mixture of two cell lines (1:1) injected into intact Balb/C mice usually produced a monoclonal tumor originating from one cell line. In most cases, the cell lines that were aggressive in vivo were also dominant in mixing experiments in vitro. In some lines, we could correlate the aggressiveness of the tumor to its superior growth rate and lower requirement for serum factors in vitro. In others, this correlation did not hold, and we assumed that host factors like the immune system contribute to the malignant potential of the leukemic cell.     

LDL oxidation is associated with increased blood hemoglobin A1c levels in diabetic patients

AIM: To investigate whether levels of blood HbA1c in diabetic patients are associated with susceptibility of LDL to oxidation. METHODS: LDL was separated from blood of 40 diabetic patients with known blood glucose and HbA1c levels. The tendency to undergo lipid peroxidation was assessed via lag time required for initiation of LDL oxidation. HbA1c formation was measured in vitro following incubation of red blood cell (RBC) hemolysate for 3 months with increasing concentrations of glucose in the absence or presence of LDL or oxidized LDL. RESULTS: Lag time for copper-induced LDL oxidation was twice as long in normal subjects compared to diabetic patients. Correlation analyses between LDL oxidation lag time and HbA1c blood levels revealed an R value of 0.74. Incubation of RBC hemolysate with high glucose concentration (up to 400 mg/dl) resulted in increased blood HbA1c concentration by up to 107%. Addition of LDL to this hemolysate over a period of 3 months resulted in LDL oxidation and an increase in HbA1c levels by up to 168%. Similarly, addition of oxidized LDL to the hemolysate increased HbA1c by up to 240%. CONCLUSIONS: Increased tendency of LDL to undergo lipid peroxidation in diabetic patients contributes to increased levels of blood HbA1c, mainly in those with HbA1c<7.3.     

Ixodid ticks infesting cattle in three agroecological zones in central Oromia: species composition,seasonal variation,and control practices

A cross-sectional study was conducted to determine the species, prevalence, and seasonal variation of ticks on cattle in central Oromia. In addition, questionnaire survey was used to assess the control practices of ticks in central Oromia. During the study period from August 2008 through April 2009, a total of 10,440 adult ixodid ticks were collected from a total of 918 cattle (153 cattle during dry and wet seasons from each of three agroecologies) in three agroecological zones during wet and dry seasons. A total of seven species of ixodid ticks belonging to four genera were identified during the wet period, whereas similar numbers of genera but six species of ixodid ticks were recorded during the dry season. Amblyomma, Boophilus, Rhipicephalus, and Hyalomma tick genera were identified during the two seasons. Five different species of ticks were identified from the highland altitude. Significantly (p?<?0.05) higher proportion of Boophilus decoloratus (88.2 %) was identified during the wet season, Hyalomma truncatum (1.5 %) was with the least proportion. Rhipicephalus evertsi evertsi (50.8 %) was the most abundant during the dry period, whereas Hyalomma marginatum rufipes (5 %) was with smallest proportion. In the midland, a total of six species of ticks were identified. Significantly (p?<?0.05) higher proportion of Amblyomma variegatum (76.6 %) than the other species was recorded during the wet season in the midland. But Rhipicephalus pulchellus (0.05 %) was collected with the lowest proportion. R. e. evertsi (46.3 %) was the most abundant tick species during the dry season, whereas R. pulchellus (0 %) was with lowest proportion. In the lowland, a total of seven species of ticks during the wet and six during the dry season were identified. A significantly (p?<?0.05) higher proportion of A. variegatum (30.3 %) was recorded during the wet season, whereas R. pulchellus (1.4 %) was identified with the lowest proportion in the lowland. R. e. evertsi (24.8 %) was the most abundant tick species during the dry season in the lowland, but R. pulchellus (0 %) was with the lowest proportion during the same season in the lowland. Results of the study revealed that the highest mean total tick burden was recorded during the early rainy season than the dry season from the end of March to April. And the lowest mean tick count was recorded during the early dry and the late dry season during December and February, respectively. The overall tick count across seasons showed significant variation (F?=?114 and p?=?0.000). The questionnaire survey on the tick control practices has revealed that (90 %) diazinon and (57.3 %) cypermethrin were the commonly used acaricides with a frequency of one to three times per year during the peak of tick infestation. In conclusion, this study demonstrated that ixodid ticks occur with high prevalence and burden and still play major roles in reducing productivity of cattle and cause health problems of cattle in central Oromia. Further studies on the role of ticks in the transmission of pathogens to cattle, other animals, and zoonotic pathogens to man in Oromia are urgently needed.