地锦草抗真菌有效部位中芦丁及槲皮苷含量的高效液相色谱法测定

目的 建立高效液相色谱(HPLC)法同时测定维吾尔医药地锦草抗真菌有效部位中芦丁及槲皮苷含量的方法.方法 色谱柱为waters symmetry C18(4.6 mm×150 mm,5μm);流动相为甲醇-0.4％磷酸水溶液(42:58,V/V),,检测波长为360nm;流速为1.0 ml· min-1;柱温25℃;进样量10μl.结果 芦丁和槲皮苷线性范围分别在0.42～2.52 μg,0.6～3.6μg内线性关系良好(r≥0.999 5),平均加样回收率分别为99.2％,101.1％,RSD≤3.3％.结论 该方法操作简便、准确、灵敏度高、重复性好,可用于地锦草抗真菌有效部位的质量控制.     

Villin 1 is a predictive factor for the recurrence of high serum alpha-fetoprotein-associated hepatocellular carcinoma after hepatectomy

The prognostic assessment of patients with hepatocellular carcinoma (HCC) after resection is an important clinical issue. The present study investigated those genes associated with high serum alpha-fetoprotein (AFP), and their clinical significance, including prognosis and recurrence after hepatectomy. Based on gene expression analysis of 110 training HCC cases, 20 genes whose mRNA expression levels were significantly upregulated and 50 genes that were downregulated correlated with high serum AFP-associated HCC patients. Gene expression profiles of Villin1 (Vil1) were obtained in high serum AFP-associated HCC tumor tissues. In the present analysis, only VIL1 was significantly correlated with the recurrence of HCC. The results were validated independently using Taqman gene expression assays and immunostaining analysis. Results showed that the upregulation of VIL1 mRNA was also correlated with high serum PIVKAII, vascular invasion (P < 0.05), poor differentiation, an advanced cancer stage (P < 0.01) and recurrence-free survival (P = 0.017). The upregulation of VIL1 mRNA was observed more frequently in the early recurrence patients as compared to the late recurrence patients. Cox regression univariate and multivariate analyses indicated that high serum AFP levels (overall survival, HR 1.675, P = 0.002; FRS, HR 1.359, P = 0.039) and Vil1 protein expression (overall survival, HR 0.253, P = 0.009; FRS, HR 0.401, P = 0.041) were independent, unfavorable prognostic factors for overall and recurrence-free survival of patients. We demonstrated that the VIL1 gene is a potential candidate molecular marker for high serum AFP-associated HCC and a predictive candidate for the postoperative recurrence and poorer prognosis of HCC.     

Selectable and Releasable Noncovalent Functionalization of Semiconducting SWCNTs by Biethynyl‐2,5‐bis(dodecoxy)benzene Unit‐Containing Conjugated Copolymers

A rigid structural unit diethynyl‐2,5‐bis(dodecoxy)benzene (DDB) is proposed to functionalize single‐walled carbon nanotubes (SWCNTs) with the assistance of pyrimidine ring. The effectiveness of conjugated polymer extraction of semiconducting SWCNTs is demonstrated by absorption and Raman spectroscopy. The protonated pyrimidine ring by the trifluoroacetic acid is used to reduce the interaction between polymer and SWCNTs that the bound wrapping polymer can be removed from the sorted SWCNTs, and molecular dynamics simulations of the binding ability of DDB and pyrimidine units to SWCNTs are conducted to further illustrate the strong binding ability of DDB units to SWCNTs. Therefore, this work provides an effective structural and theoretical reference for polymer separation or modification of semiconducting SWCNTs.     

Sorting and decoration of semiconducting single-walled carbon nanotubes via the quaternization reaction

A study for the selective separation and functionalization of alcohol-soluble semiconducting single-walled carbon nanotubes (sc-SWCNTs) is carried out by polymer main-chain engineering. Introducing tertiary amine groups endows the functionalized sc-SWCNTs with alcohol-soluble properties and introducing the pyrimidine rings allows to increase the selective purity of sc-SWCNTs. In this study, a series of poly[(9,9-dioctylfluorene)-2,7-(9,9-bis(3′-(N,N-dimethylamino)propyl)-fluorene)]_m-alt-[2-methylpyrimidine-2,7-(9,9-dioctylfluorene)]_n (PFPy) are used for the selective dispersion of semiconducting single-walled carbon nanotubes, where n and m are the composition ratio of the copolymer. When m = n, the effective isolation of sc-SWCNTs with purity greater than 99% is achieved. The alcohol-soluble sc-SWCNTs with a diameter in the range of 1.1–1.4 nm are obtained through designing reasonable molecular structure. Moreover, the particular preference of PFPy (m = n) for sc-SWCNTs was studied via density functional theory (DFT) calculations and it was proved to be a promising method for the separation and functionalization of sc-SWCNTs.

An effective route to decorate and selectively separate alcohol-soluble sc-SWCNTs has been proposed.     

含2-N,N-二甲氨基嘧啶环与噻吩环结构的共轭聚合物的合成及其性能

合成了一种新型单体2-N,N-二甲氨基-4,6-二氯嘧啶(ADNP).以二氯-1,3-双(二苯基磷)丙烷基镍(Ⅱ)作催化剂,通过2,5-二溴噻吩格氏试剂与该单体共聚得到了一种新型共轭聚合物,聚[(2-N,N-二甲胺基嘧啶-4,6-二)-2,5-噻吩](PThPm).采用FT-IR、1H-NMR等测试手段对新型单体与共聚物的结构进行了表征,并用UV-Vis、荧光光谱、X射线衍射、循环伏安、热重分析(TGA)等测试方法对单体和共聚物进行了性能分析.结果表明:共聚物在420 nm处出现UV-Vis吸收峰,在528 nm处出现荧光发射峰;共聚物的DMF溶液存在酸致变色现象,加入CF3COOH和CH3SO3H后,溶液由淡绿黄色转变为淡红色,UV-Vis吸收峰发生红移;PThPm共聚物有一定的结晶性和热稳定性;在-1.8～ 1.8 V之间出现电致变色现象,电极周围的溶液颜色从深蓝色变成深红色.     

椎间孔镜下腰椎间盘髓核摘除治疗腰椎间盘突出症的生物力学优势

文题释义：腰椎间盘突出症的手术治疗：腰椎间盘突出症是脊柱外科常见病,手术方式较多。近年来,经皮椎间孔镜腰椎间盘髓核摘除术作为微创治疗方法迅速发展,具有创伤小、出血量少并能快速康复的优点,受到越来越多的关注。 背景：腰椎间盘突出症的手术方式较多,各有优缺点,通过有限元分析方法进行脊柱生物力学研究可以指导临床工作。 目的：建立L_3-5三维有限元模型,分析不同术式在椎间盘摘除后对脊柱力学的影响。方法：选择1名无腰椎病史的35岁男性志愿者,收集腰椎CT数据构建有限元模型并验证。在L₃/L₅脊柱正常模型(模型Ⅰ)的基础上建立L_4/5节段单纯开窗髓核摘除模型(模型Ⅱ)、椎间盘镜手术模型(模型Ⅲ)、经皮椎间孔镜腰椎间盘髓核摘除模型(模型Ⅳ)、关节突关节切除模型(模型Ⅴ),在7种加载工况下测定L₄/L₅节段椎体、椎间盘和左右关节软骨应力分布及L₄椎体位移。 结果与结论：①建立的三维有限元模型有效,方差分析模型有效;②L_4-5椎间盘两两比较后模型Ⅲ、Ⅳ在椎间盘应力集中最小;L₄椎体应力,模型Ⅳ对椎体影响最小;L₄椎体位移,模型Ⅲ和Ⅳ对椎体稳定影响最小;③从加载方式看,关节突关节损伤越小,椎间盘应力越小,模型Ⅳ最小;左侧关节突模型Ⅲ和Ⅳ均保持关节完整;右侧关节左右旋转加载下手术侧关节越完整,应力越大,故模型Ⅳ较大;左右侧倾加载下反而相反,模型Ⅴ最大;L₄椎体应力模型Ⅳ最小;椎体位移,模型Ⅲ和Ⅳ位移最小,接近模型Ⅰ;④提示经皮椎间孔镜腰椎间盘髓核摘除模型在椎体、椎间盘和关节突关节应力分布最小,有明显优势;椎间盘镜手术模型和经皮椎间孔镜腰椎间盘髓核摘除模型位移均较其他手术模型稳定,二者无差异;故经皮椎间孔镜腰椎间盘髓核摘除目前是较理想的术式。　 ORCID: 0000-0002-2604-0049(吐尔洪江·阿布都热西提) 中国组织工程研究杂志出版内容重点：人工关节;骨植入物;脊柱;骨折;内固定;数字化骨科;组织工程     

一种新型哒嗪与N-戊烷基咔唑类聚合物的合成与表征

以二氯-1,3-双(二苯基磷)丙烷基镍(Ⅱ)为催化剂,通过3,6-二氯哒嗪格氏试剂与3,6-二溴-N-戊烷基咔唑共聚得到了一种新型共轭聚合物,并使用IR和1H-NMR等测试手段对单体和聚合物的结构进行了表征。该合成方法所得的共聚物收率为75%,共聚物在N,N-二甲基酰胺(DMF)、二甲基亚砜(DMSO)等极性溶剂和CF3COOH、CH3SO3H、HCOOH等常用有机酸中具有较好的溶解性。用红外(FT-IR)、紫外-可见吸收光谱(UV-vis)、荧光光谱(PL)、X射线衍射、循环伏安(CV)等对聚合物的吸收光谱特性、结晶性和电化学活性进行了探讨。结果表明:聚合物具有较高的发光强度,是一种还原型聚合物,结晶性比较差。     

主动脉瓣置换术前合并中度功能性二尖瓣关闭不全治疗策略的回顾性队列研究

目的分析主动脉瓣置换术(aortic valve replacement,AVR)同期不同方式处理中度功能性二尖瓣关闭不全(functional mitral regurgitation,FMR)对患者预后的影响。方法回顾性纳入2014～2018年在本中心接受AVR且合并中度FMR的118例患者,其中男84例、女34例,年龄(58.1±12.4)岁。根据不同二尖瓣处理方式将患者分为三组:A组(未处理,11例)、B组(二尖瓣修复,51例)及C组(二尖瓣置换,56例)。研究主要终点为患者近中期生存情况,次要终点为FMR改善情况。结果中位随访时间为29.5个月。围术期死亡5例,均为C组患者;术后早期A、B两组FMR改善率分别为90.9%、94.1%(P=0.694)。A、B、C三组中期死亡率分别为0.0%、5.9%、3.9%(P=0.264),而主要心脑血管病事件发生率分别为0.0%、9.8%、17.7%(P=0.230)。A、B两组中期FMR改善率分别为100.0%、94.3%(P>0.05)。结论对于接受AVR合并中度FMR的患者,不处理或同期修复二尖瓣更为合理,而二尖瓣置换可能会...     

Enrichment of highly pure large-diameter semiconducting SWCNTs by polyfluorene-containing pyrimidine ring

The use of copolymers to extract and purify semiconducting SWCNTs (sc-SWCNTs) and metallic SWCNTs (m-SWCNTs) is widely employed. In this paper, the performances of two pyrimidine-alt-dioctylfluorene conjugated polymers in the enrichment of semiconducting SWCNTs are compared, and the subtle structural effects on them are discussed. It was found that both pyrimidine-polymers were more effective in wrapping the semiconducting SWCNTs than the metallic SWCNTs under the co-action of the pyrimidine and fluorene rings. Moreover, methyl groups on the pyrimidine ring of the polymer slightly contributed to the semiconducting purity, and the minor differences of sc-SWCNTs extraction between two pyrimidine-polymers are compared. Additionally, the average diameter of the selected SWCNTs is larger than that of the raw SWCNTs. The experimental results show the excellent selectivity for sc-SWCNT from both co-polymers: the index Φ_i values for determining the purity of sc-SWCNTs were increased from 0.408 for P2 to 0.465 for P1, of which the selected sc-SWCNT purity is up to 99.9%. The resulting purity and the inexpensive availability of pyrimidine co-polymers make them suitable to be considered for effective sc-SWCNT enrichment.

The pyrimidine-alt-fluorene copolymer exhibits excellent selectivity towards the semiconducting SWCNTs, and the methylated pyrimidine ring presents a better performance.