全文获取类型
收费全文 | 907篇 |
免费 | 55篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 30篇 |
妇产科学 | 9篇 |
基础医学 | 129篇 |
口腔科学 | 19篇 |
临床医学 | 58篇 |
内科学 | 127篇 |
皮肤病学 | 13篇 |
神经病学 | 71篇 |
特种医学 | 89篇 |
外科学 | 85篇 |
综合类 | 14篇 |
预防医学 | 95篇 |
眼科学 | 17篇 |
药学 | 82篇 |
中国医学 | 1篇 |
肿瘤学 | 139篇 |
出版年
2022年 | 4篇 |
2021年 | 11篇 |
2020年 | 7篇 |
2019年 | 6篇 |
2018年 | 13篇 |
2017年 | 12篇 |
2016年 | 20篇 |
2015年 | 32篇 |
2014年 | 41篇 |
2013年 | 44篇 |
2012年 | 30篇 |
2011年 | 41篇 |
2010年 | 53篇 |
2009年 | 46篇 |
2008年 | 23篇 |
2007年 | 49篇 |
2006年 | 38篇 |
2005年 | 32篇 |
2004年 | 24篇 |
2003年 | 21篇 |
2002年 | 33篇 |
2001年 | 35篇 |
2000年 | 13篇 |
1999年 | 27篇 |
1998年 | 32篇 |
1997年 | 33篇 |
1996年 | 21篇 |
1995年 | 17篇 |
1994年 | 19篇 |
1993年 | 12篇 |
1992年 | 19篇 |
1991年 | 20篇 |
1990年 | 15篇 |
1989年 | 19篇 |
1988年 | 12篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1985年 | 13篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 9篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1975年 | 4篇 |
1973年 | 6篇 |
1972年 | 4篇 |
1971年 | 3篇 |
排序方式: 共有981条查询结果,搜索用时 46 毫秒
1.
Andy KH Lim 《World journal of gastroenterology : WJG》2020,26(10):1020-1028
Rhabdomyolysis is a syndrome of skeletal muscle injury with release of cellular constituents such as potassium,phosphate,urate and intracellular proteins such as myoglobin into the circulation,which may cause complications including acute kidney injury,electrolyte disturbance and cardiac instability.Abnormal liver function tests are frequently observed in cases of severe rhabdomyolysis.Typically,there is an increase in serum aminotransferases,namely aspartate aminotransferase and alanine aminotransferase.This raises the question of liver injury and often triggers a pathway of investigation which may lead to a liver biopsy.However,muscle can also be a source of the increased aminotransferase activity.This review discusses the dilemma of finding abnormal liver function tests in the setting of muscle injury and the potential implications of such an association.It delves into some of the clinical and experimental evidence for correlating muscle injury to raised aminotransferases,and discusses pathophysiological mechanisms such as oxidative stress which may cause actual liver injury.Serum aminotransferases lack tissue specificity to allow clinicians to distinguish primary liver injury from muscle injury.This review also explores potential approaches to improve the accuracy of our diagnostic tools,so that excessive or unnecessary liver investigations can be avoided. 相似文献
2.
3.
王伏虎 《南京医科大学学报(英文版)》2002,16(2):49-64
Stroke is a debilitating disease that affects millions each year.While in many cases cerebral ischemic in jury can be limited by effectivw resuscitation or thrombolytic treatment,the injured neurons wither in a process known as delayed neuronal death(DND).Mounting evidence indicates that DND is not simply necrosis played out in slow motion but apoptosis is triggered.Of particular interest are two groups of signal proteins that participate in apoptosis-cyclin dependent kinases(CDKs) and p53-among a myriad of signaling events after an ischemic insult.Recent investigations have shown that CDKs,a family of enzymes initially known for their role in cell cycle regulation,are activated in injured neurons in DND.As for p53,new reports suggest that its up-regulation may represent a failed attempt to rescue in jured neurons,although its up-regulation was previously considered an indication of apoptosis.These observations thus rekindle an old quest to identify new neuroprotective targets to minimize the stroke damage.In this review,the author will examine the evidence that indicates the participation of CDKs and p53 in DND and then introduce pre-clinical data to explore CDK inhibition as a potential neuroprotective target.Finally,using CDK inhibition as an example,this paper will discuss the pertinent criteria for a viable neuroprotective strategy for ischemic in jury. 相似文献
4.
5.
The effect of dantrolene sodium in relation to blood levels in spastic patients after prolonged administration. 总被引:1,自引:0,他引:1 下载免费PDF全文
W J Meyler H Bakker J J Kok S Agoston H Wesseling 《Journal of neurology, neurosurgery, and psychiatry》1981,44(4):334-339
In 25 patients with spasticity, pharmacokinetics and effects of dantrolene sodium were investigated after prolonged administration. A beneficial effect occurred in seven patients. The results were better on 100 mg daily than on a higher daily dose. An increase of the daily dose from 200 to 400 mg was not associated with higher blood levels. Many side effects were noted such as: anorexia, nausea, drowsiness, depression and muscle weakness. From this study we conclude that dantrolene sodium is a muscle relaxant with a weak to moderate effect in patients with spasticity; the effect at doses higher than 200 mg daily is probably poor. 相似文献
6.
7.
Androgen receptor YAC transgenic mice carrying CAG 45 alleles show trinucleotide repeat instability 总被引:1,自引:15,他引:1
La Spada AR; Peterson KR; Meadows SA; McClain ME; Jeng G; Chmelar RS; Haugen HA; Chen K; Singer MJ; Moore D; Trask BJ; Fischbeck KH; Clegg CH; McKnight GS 《Human molecular genetics》1998,7(6):959-967
X-linked spinal and bulbar muscular atrophy (SBMA) is caused by a CAG
repeat expansion in the first exon of the androgen receptor (AR) gene.
Disease-associated alleles (37-66 CAGs) change in length when transmitted
from parents to offspring, with a significantly greater tendency to shift
size when inherited paternally. As transgenic mice carrying human AR cDNAs
with 45 and 66 CAG repeats do not display repeat instability, we attempted
to model trinucleotide repeat instability by generating transgenic mice
with yeast artificial chromosomes (YACs) carrying AR CAG repeat expansions
in their genomic context. Studies of independent lines of AR YAC transgenic
mice with CAG 45 alleles reveal intergenerational instability at an overall
rate of approximately 10%. We also find that the 45 CAG repeat tracts are
significantly more unstable with maternal transmission and as the
transmitting mother ages. Of all the CAG/CTG repeat transgenic mice
produced to date the AR YAC CAG 45 mice are unstable with the smallest
trinucleotide repeat mutations, suggesting that the length threshold for
repeat instability in the mouse may be lowered by including the appropriate
flanking human DNA sequences. By sequence-tagged site content analysis and
long range mapping we determined that one unstable transgenic line has
integrated an approximately 70 kb segment of the AR locus due to
fragmentation of the AR YAC. Identification of the cis - acting elements
that permit CAG tract instability and the trans -acting factors that
modulate repeat instability in the AR YAC CAG 45 mice may provide insights
into the molecular basis of trinucleotide repeat instability in humans.
相似文献
8.
Keratitis caused by Scedosporium apiospermum successfully treated with a cornea transplant and voriconazole 总被引:2,自引:0,他引:2 下载免费PDF全文
Nulens E Eggink C Rijs AJ Wesseling P Verweij PE 《Journal of clinical microbiology》2003,41(5):2261-2264
A case of Scedosporium apiospermum keratitis was successfully treated with oral voriconazole and penetrating keratoplasty. Voriconazole levels in the aqueous humor were 53% of the levels in plasma and exceeded the MIC for the isolate by sevenfold. 相似文献
9.
Founder effect in spinal and bulbar muscular atrophy (SBMA) 总被引:3,自引:2,他引:3
Tanaka F; Doyu M; Ito Y; Matsumoto M; Mitsuma T; Abe K; Aoki M; Itoyama Y; Fischbeck KH; Sobue G 《Human molecular genetics》1996,5(9):1253-1257
We analyzed the polymorphic (CAG)n and (GGC)n repeats of the androgen
receptor gene in 113 unrelated X-linked spinal and bulbar muscular atrophy
(SBMA) X chromosomes and 173 control X chromosomes in Japanese males. The
control chromosomes had an average CAG repeat number of 21 +/- 3 with a
range from 14-32 repeat units, and SBMA chromosomes had a range from 40-55
with a median of 47 +/- 3 copies. The control chromosomes had seven
different alleles of the (GGC)n repeat with the range of 11 to 17; the most
frequent size of (GGC)n was 16 (79%), while (GGC)17 was very rare (1%).
However, in SBMA chromosomes only two alleles were seen; the most frequent
size of (GGC)n was 16 (61%) followed by 17 (39%). (GGC)n size distribution
was significantly different between SBMA and control chromosomes (P <
0.0001), indicating the presence of linkage disequilibrium. There was no
allelic association between the (CAG)n and (GGC)n microsatellites among
control subjects as well as SBMA patients, which suggests that a founder
effect makes a more significant contribution to generation of Japanese SBMA
chromosomes than new mutations.
相似文献
10.
Agrin is a major heparan sulfate proteoglycan accumulating in Alzheimer's disease brain 总被引:4,自引:0,他引:4 下载免费PDF全文
Verbeek MM Otte-Höller I van den Born J van den Heuvel LP David G Wesseling P de Waal RM 《The American journal of pathology》1999,155(6):2115-2125
Heparan sulfate proteoglycans (HSPGs) have been suggested to play an important role in the formation and persistence of senile plaques and neurofibrillary tangles in dementia of the Alzheimer's type (DAT). We performed a comparative immunohistochemical analysis of the expression of the HSPGs agrin, perlecan, glypican-1, and syndecans 1-3 in the lesions of DAT brain neocortex and hippocampus. Using a panel of specific antibodies directed against the protein backbone of the various HSPG species and against the glycosaminoglycan (GAG) side-chains, we demonstrated the following. The basement membrane-associated HSPG, agrin, is widely expressed in senile plaques, neurofibrillary tangles and cerebral blood vessels, whereas the expression of the other basement membrane-associated HSPG, perlecan, is lacking in senile plaques and neurofibrillary tangles and is restricted to the cerebral vasculature. Glypican and three different syndecans, all cell membrane-associated HSPG species, are also expressed in senile plaques and neurofibrillary tangles, albeit at a lower frequency than agrin. Heparan sulfate GAG side chains are also associated with both senile plaques and neurofibrillary tangles. Our results suggest that glycosaminoglycan side chains of the HSPGs agrin, syndecan, and glypican, but not perlecan, may play an important role in the formation of both senile plaques and neurofibrillary tangles. In addition, we speculate that agrin, because it contains nine protease-inhibiting domains, may protect the protein aggregates in senile plaques and neurofibrillary tangles against extracellular proteolytic degradation, leading to the persistence of these deposits. 相似文献