非脱垂子宫经阴道全切除术在肥胖患者中的应用

目的:比较肥胖和非肥胖患者经阴道子宫全切除术的手术结局。方法:因子宫良性病变行子宫全切除者,BM I≥30 kg/m2者为研究组,BM I<30 kg/m2者为对照组。比较两组的出血量、手术时间、排气时间、住院时间、发热率、子宫重量及其他并发症的发生情况。结果:研究组和对照组出血量分别为(184.6±82.7)m l及(179.8±78.2)m l,出血量超过500 m l而需要输血者研究组及对照组分别为4.8%(2例)及4.1%(8例),手术时间分别为(87.4±23.6)m in及(85.9±25.1)m in,排气时间分别为(16.4±3.6)h及(17.1±4.2)h,住院时间分别为(5.7±1.2)天及(5.5±1.1)天,发热率分别为7.1%(3例)及6.1%(12例),子宫重量分别为(261.5±52.8)g及(258.3±48.6)g(P>0.05)。结论:经阴道子宫全切除术可安全应用于肥胖患者。     

Nontraumatic posterior atlantooccipital dislocation associated with atlantoaxial instability

Introduction

Nontraumatic posterior atlantooccipital dislocation has only been rarely reported. In the current study, the authors reported an extremely rare case of nontraumatic posterior atlantooccipital dislocation associated with atlantoaxial instability.

Materials and methods

A 47-year-old female was referred with a history of neck pain for 5 years. The patient had no history of trauma. The axial rotation of range of motion of the cervical spine was severely restricted. Posterior atlantooccipital dislocation with atlantoaxial instability was confirmed through conventional radiography, computed tomography and magnetic resonance imaging. We performed realignment of the dislocation and posterior occipitocervical (C0–C2) fusion. After the surgery, the patient’s symptoms improved significantly and she manifested neurological improvement.

Conclusion

To our knowledge, this lesion has not been reported previously. Anomalies of upper cervical spine may have induced this instability.

     

非脱垂子宫经阴道全切除术150例临床分析

目的:观察非脱垂子宫经阴道全切除术的临床结局及其影响因素。方法:比较不同大小子宫及不同部位子宫肌瘤对手术时间、出血量及术后发热率的影响。结果:子宫≤8孕周与>8孕周者相比较,手术时间及阴道出血量前者小于后者(P<0.05),术后发热率两组相比较差异无显著性(P>0.05),子宫肌瘤位于子宫上段者与位于下段者相比较,手术时间及出血量前者均小于后者(P<0.01),术后发热率两组相比较差异无显著性(P>0.05)。结论:子宫大小及子宫肌瘤部位对非脱垂子宫经阴道全切除术手术时间及出血量具有明显影响,对发热率无影响。     

Chemical composition, antimicrobial and antioxidant activities of essential oil from Gnaphlium affine

The chemical composition of the essential oil from Gnaphlium affine was determined, and its antimicrobial and antioxidant activities were evaluated. Twenty-four compounds, representing 94.95% of the amount of total oil, were identified by gas chromatography-mass spectrometry (GC-MS) analysis. Main constituents of the essential oil were found to be eugenol (18.24%), linalool (10.62%), trans-caryophyllene (8.86%), α-terpineol (5.97%), p-cymene (5.75%), hexadecanoic acid (5.63%), γ-cadinene (4.98%), δ-cadinene (4.22%), α-humulene (3.22%), and (−)-β-elemene (3.15%). The essential oil revealed a remarkable antimicrobial effect against the tested food-borne microorganisms with the MIC and MBC values in the ranges of 0.2-1.56 μg/ml and 0.39-3.13 μg/ml, respectively. The essential oil showed a potent antioxidant activity in ABTS radical scavenging, lipid peroxidation and reducing power assay. It was suggested that the essential oil from G. affine may be a new potential source as natural antimicrobial and antioxidant agents applied in food systems.     

Subcutaneous and intranasal immunization with Stx2B-Tir-Stx1B-Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice

The type III secretion system of Escherichia coli O157:H7 is involved in colonization of mammalian hosts by the organism. The translocated intimin receptor (Tir) is inserted into the mammalian host cell plasma membrane in a hairpin loop topology with the central loop of the molecule exposed to the host cell surface and accessible for interaction with an LEE-encoded bacterial outer membrane adhesin called intimin. Shiga toxin type 1 and 2 produced by E. coli O157:H7 are responsible for hemolytic uremic syndrome and able to promote intestinal colonization. Zonula occludens toxin (Zot) is a single polypeptide chain encoded by the filamentous bacteriophage CTXφ of Vibrio cholerae. Zot binds a receptor on intestinal epithelial cells and increases mucosal permeability by affecting the structure of epithelial tight junctions. Because of these properties, Zot is a promising tool for mucosal drug and antigen (Ag) delivery. In the current study, we constructed a novel fusion protein carrying both of the immunogenic B subunits derived from the two toxins, Tir and Zot, designated Stx2B-Tir-Stx1B-Zot, expressed in the E. coli BL21 and harvested the purified protein by a simple GST·Bind Resin chromatography method. We used a streptomycin-treated mouse model to evaluate the efficacy of subcutaneous vs. intranasal administration of the vaccine. Following immunization, mice were infected with E. coli O157:H7 and feces were monitored for shedding. Immune responses against Stx2B-Tir-Stx1B-Zot, Stx2B-Tir-Stx1B and control agent (GST/PBS) were also monitored. Subcutaneous immunization of mice with Stx2B-Tir-Stx1B-Zot induced significant Stx2B-Tir-Stx1B-Zot-specific serum IgG antibodies but did not significantly induce any antigen-specific IgA in feces, whereas intranasal immunization elicited significant Stx2B-Tir-Stx1B-Zot-specific serum IgG antibodies with some animals developing antigen-specific IgA in feces. Mice that were immunized intranasally with Stx2B-Tir-Stx1B-Zot showed dramatically decreased E. coli O157:H7 shedding compared to those of Stx2B-Tir-Stx1B and control agent following experimental infection. Mice immunized subcutaneously with Stx2B-Tir-Stx1B-Zot or Stx2B-Tir-Stx1B both showed reduced shedding in feces, moreover, Stx2B-Tir-Stx1B-Zot did better. These results demonstrate the perspective for the use of Stx2B-Tir-Stx1B-Zot to prevent colonization and shedding of E. coli O157:H7.     

急性特发性血小板减少性紫癜儿童T-bet和GATA3的表达

目的：通过分析急性特发性血小板减少性紫癜（ITP）儿童血中转录因子T-bet和GATA3 mRNA的表达及细胞因子IFN-γ和IL-4的含量,探讨急性ITP发病机制中Th1/Th2细胞分化的趋势及其与转录因子的关系。方法：利用RT-PCR技术,测定急性TIP患儿发作期（n=30）和缓解期（n=28）外周血中T-bet和GATA3 mRNA 的表达,并使用ELISA法检测血浆IFN-γ和IL-4含量。20例健康儿童作为对照组。结果：ITP患儿发作期血中T-bet mRNA表达和IFN-γ含量明显高于缓解期和对照组（P＜0.01）。发作期血中GATA3 mRNA表达和IL-4的含量明显低于缓解期和对照组（P＜0.01）。结论：儿童急性ITP存在明显的Th1优势分化现象,且可能与转录因子T-bet和GATA3的调控作用有关。［中国当代儿科杂志,2010,12（1）：29-31］     

Emerging role of ER quality control in plant cell signal perception

The endoplasmic reticulum quality control (ER-QC) is a conserved mechanism in surveillance of secreted signaling factors during cell-to-cell communication in eukaryotes.Recent data show that the ER-QC ...     

Mechanism of induction of fibroblast to corneal endothelial cell

Objective:To explore mechanism of nduction of fibroblast to corneal endothelial cell.Methods:Rabbit conjunctiva fibroblasts were used as feeder cells,rabbit oral mucosa epithelial cells were used as seed cells,and human denuded amniotic membrane was used as carrier to establish tissue engineering corneal endothelium.The transformation effect was observed.Results:As concentration of mitomycin C increased,cell survival rale gradually decreased,cell proliferation was obviously inhibited when concentration ≥25μg/mL;5 days alter being treated by 5μg/mL.mitomycin C,cell body was enlarged and extended without cell fusion,however after being treated by 0.5 μg/mL mitomycin C,cell body was significantly proliferated and gradually fused:alter 3 weeks of culture,stratified epithelium appeared on rabbit oral mucosa epithelial cells,differentiation layers were 4-5 and were well differentiated,the morphology was similar to corneal endothelial cells;Under electron microscope,surface layer of cells were polygonal,tightly connected to another with microvilli on the bonier,there was hemidesmosome between basal cells and human denuded amniotic membrane.Conclusions:Fibroblast cells have the potential of multi-directional differentiation,effective induction can promote emergence of intercellular desmosomes between seed cells and emergence ol epithelial surface microvilli,and differentiate to the corneal endothelial cell.However,clinical application still needs more research and safetv evaluation.     

3D-printed custom trays with a Gothic arch for centric relation recording and definitive impression making for complete dentures: A dental technique

An appliance was designed and fabricated by using computer-aided design and computer-aided manufacturing (CAD-CAM) and 3-dimensional (3D) printing to combine a custom tray with an intraoral Gothic arch. This helped simplify centric relation recording and combined definitive impression making and centric relation recording into a single step.