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目的 真核表达人呼吸道合胞病毒(human respiratory syncytial virus,SV)融合蛋白(fusion protein,),并完成蛋白纯化及纯度测定.方法 根据编码F蛋白的基因序列设计引物,CR方法扩增出3'端带His标签的F基因序列,克隆入pGEM-T-easy载体,经核酸序列分析后,进一步克隆到pcDNA3.1( )真核表达载体,限制性内切酶鉴定,用脂质体Lipofectamine2000转染COS-7细胞,2 h后再用Westem blot检测目的蛋白的表达.Ni柱亲和层析纯化COS-7细胞表达的F蛋白,高效毛细管电泳分析纯化后蛋白纯度.结果 核酸序列分析证实获得带His标签的RSV F基因序列,没有发生无义突变.转染COS-7细胞后,利用Western blot方法检测到F蛋白的特异性条带,纯度达99%以上.结论 初步建立了真核表达RSV F蛋白的纯化方法,为进一步优化RSV F蛋白制备条件及单克隆抗体及诊断试剂等研究奠定了基础. 相似文献
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Effects of Exercise and Respiration on Hemodynamic Efficiency in CFD Simulations of the Total Cavopulmonary Connection 总被引:1,自引:0,他引:1
Marsden AL Vignon-Clementel IE Chan FP Feinstein JA Taylor CA 《Annals of biomedical engineering》2007,35(2):250-263
Congenital heart defects with a single functional ventricle, such as hypoplastic left heart syndrome and tricuspid atresia,
require a staged surgical approach to separate the systemic and pulmonary circulations. Ultimately, the venous or pulmonary
side of the heart is bypassed by directly connecting the vena cava to the pulmonary arteries with a modified t-shaped junction.
The Fontan procedure (total cavopulmonary connection, TCPC) completes this process of separation. To date, computational fluid
dynamics (CFD) simulations in this low pressure, passive flow, intrathoracic system have neglected the presumed important
effects of respiration on physiology and higher “stress” states such as with exercise have never been considered. We hypothesize
that incorporating effects of respiration and exercise would provide more realistic estimates of TCPC performance. Time-dependent,
3D blood flow simulations are performed by a custom finite element solver for two patient-specific Fontan models with a novel
respiration model, developed to generate physiologic time-varying flow conditions. Blood flow features, pressure, and energy
efficiency are analyzed at rest and with increasing flow rates to simulate exercise conditions. The simulations produce realistic
pressure and flow data, comparable to that measured by catheterization and echocardiography, and demonstrate substantial increases
in energy dissipation (i.e. decreased performance) with exercise and respiration due to increasing intensity of small scale
vortices in the flow. As would be expected, these changes are highly dependent on patient-specific anatomy and Fontan geometry.
We propose that respiration and exercise should be incorporated into TCPC CFD simulations to provide increasingly realistic
evaluations of TCPC performance. 相似文献
5.
Mohamed Bekheit Chloe Audebert Petru Bucur Hans Adriaensen Emilie Bled Mylène Wartenberg Irene Vignon-Clementel Eric Vibert 《Hepatobiliary & pancreatic diseases international : HBPD INT》2018,17(6):538-545
Background: The hepatic hemodynamics is an essential parameter in surgical planning as well as in various disease processes. The transit time ultrasound(TTUS) perivascular flow probe technology is widely used in clinical practice to evaluate the hepatic inflow, yet invasive. The phase-contrast-MRI(PC-MRI) is not invasive and potentially applicable in assessing the hepatic blood flow. In the present study, we compared the hepatic inflow rates using the PC-MRI and the TTUS probe, and evaluated their predictive value of post-hepatectomy adverse events. Methods: Eighteen large white pigs were anaesthetized for PC-MRI and approximately 75% hepatic resection was performed under a unified protocol. The blood flow was measured in the hepatic artery(Qha), the portal vein(Qpv), and the aorta above the celiac trunk(Qca) using PC-MRI, and was compared to the TTUS probe. The Bland-Altman method was conducted and a partial least squares regression(PLS) model was implemented. Results: The mean Qpv measured in PC-MRI was 0.55 ± 0.12 L/min, and in the TTUS probe was 0.74 ± 0.17 L/min. Qca was 1.40 ± 0.47 L/min in the PC-MRI and 2.00 ± 0.60 L/min in the TTUS probe. Qha was 0.17 ± 0.10 L/min in the PC-MRI, and 0.13 ± 0.06 L/min in the TTUS probe. The Bland-Altman method revealed that the estimated bias of Qca in the PC-MRI was 32%(95% CI:-49% to 15%); Qha 17%(95% CI:-15% to 51%); and Qpv 40%(95% CI:-62% to 18%). The TTUS probe had a higher weight in predicting adverse outcomes after 75% resection compared to the PC-MRI( β= 0.35 and 0.43 vs β = 0.22 and 0.07, for tissue changes and premature death, respectively). Conclusions: There is a tendency of the PC-MRI to underestimate the flow measured by the TTUS probes. The TTUS probe measures are more predictive of relevant post-hepatectomy outcomes. 相似文献
6.
目的:探讨上皮样血管瘤(EH)的临床及组织病理学特征。 方法:回顾性分析16例已确诊患者的临床及病理资料,并对相关文献进行综述。 结果:16例患者中女8例,男8例;平均年龄(45.44±12.52)岁;病程3个月~20年;皮损主要表现为红色至暗红色丘疹、结节,可伴瘙痒和糜烂,好发于头皮、耳部,其中发生在头部10例、耳部5例、外阴1例;皮损单发者5例,多发者11例。皮损组织病理学检查均具有典型的血管增生,管壁增厚,内皮细胞呈上皮样突向管腔,管周大量淋巴细胞及嗜酸性粒细胞浸润。结论:上皮样血管瘤少见,诊断需要临床与病理密切结合。加强对该病的认识,可避免误诊误治。 相似文献
7.
We measured total respiratory system compliance (Crs) before and after instilling 25 mg artificial surfactant in 1 ml saline down the endotracheal tube of preterm babies requiring resuscitation at birth, and compared results with data from 6 similar babies receiving saline only. Surfactant did not produce a significant improvement in Crs. 相似文献
8.
DI Wilson SB Britton C McKeown D Kelly IE Cross S Strobel PJ Scambler 《Archives of disease in childhood》1993,68(2):187-189
A boy with the dysmorphic features of Noonan's syndrome and pulmonary valve stenosis who had evidence of hypoparathyroidism and abnormal T lymphocyte numbers in the neonatal period is reported. He had a normal karyotype but molecular analysis revealed a submicroscopic deletion within chromosome 22q11, the region deleted in DiGeorge syndrome. Thus this child has both Noonan's syndrome and DiGeorge syndrome; 22q11 is a candidate region for a gene defective in Noonan's syndrome. 相似文献
9.
H. J. Kim I. E. Vignon-Clementel C. A. Figueroa J. F. LaDisa K. E. Jansen J. A. Feinstein C. A. Taylor 《Annals of biomedical engineering》2009,37(11):2153-2169
Aortic flow and pressure result from the interactions between the heart and arterial system. In this work, we considered these
interactions by utilizing a lumped parameter heart model as an inflow boundary condition for three-dimensional finite element
simulations of aortic blood flow and vessel wall dynamics. The ventricular pressure–volume behavior of the lumped parameter
heart model is approximated using a time varying elastance function scaled from a normalized elastance function. When the
aortic valve is open, the coupled multidomain method is used to strongly couple the lumped parameter heart model and three-dimensional
arterial models and compute ventricular volume, ventricular pressure, aortic flow, and aortic pressure. The shape of the velocity
profiles of the inlet boundary and the outlet boundaries that experience retrograde flow are constrained to achieve a robust
algorithm. When the aortic valve is closed, the inflow boundary condition is switched to a zero velocity Dirichlet condition.
With this method, we obtain physiologically realistic aortic flow and pressure waveforms. We demonstrate this method in a
patient-specific model of a normal human thoracic aorta under rest and exercise conditions and an aortic coarctation model
under pre- and post-interventions. 相似文献
10.