继发性外部性脑积水的CT诊断及随访观察

目的探讨继发性外部性脑积水的CT特征及随访观察的价值.方法回顾性分析了36例有明确病因的继发性外部性脑积水的CT特征及随访结果.结果双侧额或额顶部蛛网膜下腔对称性增宽者36例(100%),双侧裂池增宽者36例(100%),额顶部脑沟增宽加深者31例(86.11%),前纵裂池增宽者36例(100%),脑基底池扩大者21例(58.33%),脑室轻度扩大者7例(19.44%).复查10例患儿其中8例外部性脑积水及临床症状消失,智力良好.其余2例均伴外伤史,其中一例复查出现脑萎缩征象,另一例出现脑积水征象.结论C检查及随访观察对于继发性外部性脑积水的诊断、鉴别诊断及指导临床治疗具有重要的价值.     

平板运动试验Q-Tc、Q-Tr和△Q-Tc对冠心病的诊断价值:与冠脉造影结果对照

78例病人接受冠脉造影和次极量平板运动试验,探讨Q-Tc、Q-Tr和△Q-Tc预测冠脉病变的准确性。Q-Tc与Q-Tr的敏感度,特异度,阳性预测值及诊断准确率分别为84,76,83,81％与69,88,89,77％,与ST段降低的相比无明显差别,提示其诊断价值相似。与心血管造影资料对照,上述指标能反映冠脉病变的程度。△Q-Tc的敏感度低(58％)而特异性高(94％),其诊断准确性(73％)与其他指标相比无显著差异,故有应用价值。     

脑膜瘤组织中Survivin和PCNA的表达

目的 探讨Survivin和PCNA在脑膜瘤组织中的表达及临床意义。方法 采用免疫组织化学sP法检测97例脑膜瘤组织和18例正常脑膜组织中Survivin和PCNA的表达。结果 Survivin在正常脑膜和脑膜瘤组织中阳性表达率为0％和65．9％（64／97），差异具有显著性（P〈0．001）。Survivln表达阳性率在脑膜瘤Ⅱ级（85．7％）和Ⅰ级（51、7％）之间、Ⅲ级（93．8％）和Ⅰ级之间差异具有显著性（P〈0．001）。PCNA蛋白在正常脑膜和脑膜瘤组织中表达阳性率为0％和55．7％（54／97），差异具有显著性（P〈0．001）。PC—NA表达阳性率在脑膜瘤Ⅱ级（71．4％）和Ⅰ级（41、7％）之间，Ⅲ级（87．5％）同Ⅰ级之间差异具有显著性（P〈0．001）。Survivin与PCNA蛋白在脑膜瘤中的表达呈正相关关系（rs=0．640，P〈0．01）。结论 Survivin过度表达和脑膜瘤发生及PCNA的表达密切相关，两者参与了脑膜瘤发生、增殖和发展。     

Mouse chromosome 9 quantitative trait loci for soft tissue regeneration: Congenic analysis and fine mapping

Development of gene therapies for wound healing will depend on the identification of the genes involved in wound healing and tissue regeneration. Previous quantitative trait loci (QTL) studies in mice using the ear punch model have shown that major QTL exist on chromosome (Chr) 9 for soft tissue regeneration. In this study, we have developed a congenic line that contains the Chr 9 QTL chromosomal region from super healer MRL/MpJ in the genomic background of poor-healing SJL/J. The phenotypic effect of this QTL was confirmed in male mice, where the congenic line has shown significant healing improvement over SJL. Fine mapping of the Chr 9 QTL region with 23 markers at an average distance of 4.2 Mb using a total of 1,564 MRL/MpJ x SJL/J F(2) mice revealed the presence of at least three QTL peaks, implying that three separate loci may contribute to the phenotypic effect of this QTL. Based on the 2-LOD intervals, the total QTL region was confined to a combined length of no more than 28.2 Mb. Application of a Bayesian shrinkage estimation indicated that a major locus was located in a region of just 1.3 Mb.     

微电极导向同期双侧腹后苍白球 毁损术治疗帕金森病

目的介绍微电极导向同期双侧腹后苍白球毁损术治疗帕金森病的方法与效果。方法应用微电极电生理记录技术术对毁损靶点进行确认定位,对３１例难治性帕金森病患者行同期双侧腹后内侧苍白球毁损治疗,术前及术后于开状态、关状态分别行改良Ｗｅｂｓｔｅｒ记分,计算改善率,评价其疗效。结果经微电极确认后的电生理靶点与ＣＴ定位靶点存在明显差异,靶点调整率８０６％。３１例手术均有效,其中治愈７例,明显进步２４例。Ｗｅｂｓｔｅｒ计分术后１周开状态改善率为７４２％±９５％,关状态改善率为８９１％±８９％。无永久性并发症。结论微电极导向同期双侧腹后苍白球毁损术安全、有效,具有明显临床治疗优势,微电极记录技术可使术中定位精确度大大提高。     

Construction and characterization of a cigR deletion mutant of Salmonella enterica serovar Pullorum

Salmonella enterica serovar Pullorum (S. Pullorum) is the causative agent of pullorum disease (PD) and results in severe economic losses to the poultry industry. As a Salmonella type III secretion system 2 (T3SS2) effector and predicted membrane protein, CigR is encoded by the cigR gene within Salmonella pathogenicity island 3 (SPI3). In order to research the influence of the cigR gene on S. Pullorum, a cigR mutant of S. Pullorum S06004 was constructed by the lambda Red recombination system, and then its characterization was analysed. Lack of cigR did not affect the growth and biochemical properties, but resulted in decreased biofilm formation. The mutant strain was stable with the deletion of the cigR gene. Macrophage infection assay and in vivo competition assay showed that the mutant strain increased the replication and/or survival ability in the HD11 cell line and in chickens compared to that of the parent strain, the median lethal dose (LD₅₀) of the mutant strain was one-fifth of the parent strain for 2-day-old chickens when injected intramuscularly. These results demonstrate CigR plays roles in biofilm formation and pathogenicity of S. Pullorum, deletion of cigR can significantly decrease biofilm formation and significantly increase virulence.     

基于BP神经网络和遗传算法优化茺蔚子水苏碱提取工艺的研究

【目的】优化茺蔚子中水苏碱的提取工艺。【方法】采用正交设计,应用误差反向后传（BP）人工神经网络建立茺蔚子水苏碱提取时间、乙醇浓度、料液比与盐酸水苏碱含量之间的关系模型,再结合遗传算法优化提取工艺参数。【结果】提取工艺优化参数为：提取时间62 min、乙醇浓度体积分数69%、料液比11倍,网络模型优化结果稍优于正交试验结果。【结论】 BP神经网络结合遗传算法寻优,能较好地实现茺蔚子水苏碱提取工艺参数的优化,可为解决多维非线性系统的工艺参数优化问题提供崭新而有效的途径。     

从少阳经辨证论治眩晕症的探讨

眩晕作为临床常见症状,可见于现代医学的多种病症,常常涉及到神经科、眼科、耳鼻喉科、心血管科等多种学科.中医病因一般为肝阳上亢、气血亏虚、痰湿中阻、肾精不足四种,即"风、火、痰、虚"四个方面.临床辨证论治以脏腑辨证为多见,也有以经络辨证治疗.作者从少阳经论治眩晕,做了有益的探讨,在临床上取得了一定的临床效果.     

Evaluation of the Salmonella enterica Serovar Pullorum Pathogenicity Island 2 Mutant as a Candidate Live Attenuated Oral Vaccine

Salmonella enterica serovar Pullorum (S. Pullorum) is a highly adapted pathogen that causes pullorum disease (PD), an important systemic disease of poultry that causes severe economic losses in developing countries. In the interests of developing a safe and immunogenic oral vaccine, the efficacy of a Salmonella pathogenicity island 2 (SPI2)-deleted mutant of S. Pullorum (S06004ΔSPI2) was evaluated in chickens. S06004ΔSPI2 was severely less virulent than the parental wild-type strain S06004 as determined by the 50% lethal dose (LD₅₀) for 3-day-old chickens when injected intramuscularly. Two-day-old chickens immunized with a single oral dose of S06004ΔSPI2 showed no differences in body weight or clinical symptoms compared with those in the negative-control group. S06004ΔSPI2 bacteria were not isolated from livers or spleens of immunized chickens after a short period of time, and specific humoral and cellular immune responses were significantly induced. Immunized chickens were challenged with S. Pullorum strain S06004 and Salmonella enterica serovar Gallinarum (S. Gallinarum) strain SG9 at 10 days postimmunization (dpi), and efficient protection against the challenges was observed. None of the immunized chickens died, the clinical symptoms were slight and temporary following challenge in immunized chickens compared with those in the control group, and these chickens recovered by 3 to 5 dpi. Overall, these results demonstrate that S06004ΔSPI2 can be used as a live attenuated oral vaccine.     

瞬时受体电位通道蛋白6参与血管紧张素Ⅱ诱导足细胞损伤的信号传导机制

足细胞表达瞬时受体电位通道蛋白6(TRPC6),TRPC家族是一类能通透钙离子的非选择性阳离子通道,近年来,研究发现TRPC6参与了血管紧张素Ⅱ(AngⅡ)诱导足细胞损伤的病理生理过程.本文就TRPC6一般特性及参与AngⅡ诱导足细胞损伤的信号传导机制作一简述.