全文获取类型
收费全文 | 1582篇 |
免费 | 91篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 64篇 |
妇产科学 | 20篇 |
基础医学 | 191篇 |
口腔科学 | 18篇 |
临床医学 | 191篇 |
内科学 | 266篇 |
皮肤病学 | 36篇 |
神经病学 | 124篇 |
特种医学 | 81篇 |
外科学 | 335篇 |
综合类 | 21篇 |
一般理论 | 2篇 |
预防医学 | 85篇 |
眼科学 | 73篇 |
药学 | 87篇 |
肿瘤学 | 86篇 |
出版年
2024年 | 2篇 |
2023年 | 26篇 |
2022年 | 26篇 |
2021年 | 59篇 |
2020年 | 38篇 |
2019年 | 54篇 |
2018年 | 51篇 |
2017年 | 34篇 |
2016年 | 51篇 |
2015年 | 75篇 |
2014年 | 79篇 |
2013年 | 109篇 |
2012年 | 158篇 |
2011年 | 169篇 |
2010年 | 88篇 |
2009年 | 73篇 |
2008年 | 120篇 |
2007年 | 69篇 |
2006年 | 90篇 |
2005年 | 81篇 |
2004年 | 64篇 |
2003年 | 60篇 |
2002年 | 34篇 |
2001年 | 8篇 |
2000年 | 9篇 |
1999年 | 10篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有1683条查询结果,搜索用时 15 毫秒
1.
2.
3.
Wound healing in diabetes mellitus 总被引:1,自引:0,他引:1
Diabetes mellitus is a complex metabolic disorder whose components have several direct and indirect effects on the healing of wounds. This article examines how diabetes affects wound healing from the standpoint of alterations in the microvasculature, nerve function, and the immune system. 相似文献
4.
5.
Clone-based systematic haplotyping (CSH): a procedure for physical haplotyping of whole genomes 总被引:4,自引:0,他引:4
下载免费PDF全文
![点击此处可从《Genome research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Burgtorf C Kepper P Hoehe M Schmitt C Reinhardt R Lehrach H Sauer S 《Genome research》2003,13(12):2717-2724
We present a novel methodology to determine the phase of single-nucleotide polymorphisms (SNPs) on a chromosome, which we term clone-based systematic haplotyping (CSH). The CSH procedure is based on separating the allelic chromosomes of a diploid genome by fosmid/cosmid cloning, and subsequent SNP typing of 96 clone pools, each representing approximately 10% of the genome. The pools are screened by PCR for the sequence of interest, followed by SNP typing on the PCR products using the GOOD assay. We demonstrate that by CSH, the haplotype of SNPs separated by more than 50 kilobases can definitely be assigned. We propose this method as being suitable for constructing maps of ancestral haplotypes, analysis of complex diseases, and for diagnosis of rare defects in which the molecular haplotype is crucial. In addition, by amplifying the initial DNA by many orders of magnitude, the original DNA resource is effectively immortalized, enabling the haplotyping of hundreds of thousands of SNPs per individual. 相似文献
6.
Lena Möbus Elke Rodriguez Inken Harder Agatha Schwarz Ulrike Wehkamp Dora Stölzl Nicole Boraczynski Sascha Gerdes Thomas Litman Andreas Kleinheinz Susanne Abraham Annice Heratizadeh Christiane Handrick Eva Haufe Jochen Schmitt Thomas Werfel Stephan Weidinger 《The Journal of allergy and clinical immunology》2021,147(5):1959-1965.e2
7.
8.
Hordijk José A. Verbruggen Sascha C. Buysse Corinne M. Utens Elisabeth M. Joosten Koen F. Dulfer Karolijn 《Quality of life research》2022,31(9):2615-2617
Quality of Life Research - 相似文献
9.
Asher Y. Rosinger Samantha M. Olson Sascha R. Ellington Janice Perez-Padilla Regina M. Simeone Caitlin S. Pedati Betsy A. Schroeder Gilberto A. Santiago Freddy A. Medina Jorge L. Muoz-Jordn Laura E. Adams Romeo R. Galang Miguel Valencia-Prado Sonia Bakkour Candimar Coln Mary Goodwin Dana Meaney-Delman Jennifer S. Read Lyle R. Petersen Denise J. Jamieson Carmen C. Deseda Margaret A. Honein Brenda Rivera-García Carrie K. Shapiro-Mendoza 《Emerging infectious diseases》2021,27(5):1505
We evaluated nucleic acid amplification testing (NAAT) for Zika virus on whole-blood specimens compared with NAAT on serum and urine specimens among asymptomatic pregnant women during the 2015–2016 Puerto Rico Zika outbreak. Using NAAT, more infections were detected in serum and urine than in whole blood specimens. 相似文献
10.
Carolin Lepa Sascha Hoppe Antje Stber Boris V. Skryabin Laura Katharina Sievers Barbara Heitplatz Giuliano Ciarimboli Ute Neugebauer Maja T. Lindenmeyer Clemens D. Cohen Hannes C.A. Drexler Peter Boor Thomas Weide Hermann Pavenstdt Britta George 《Journal of the American Society of Nephrology : JASN》2021,32(2):357
BackgroundInjury to kidney podocytes often results in chronic glomerular disease and consecutive nephron malfunction. For most glomerular diseases, targeted therapies are lacking. Thus, it is important to identify novel signaling pathways contributing to glomerular disease. Neurotrophic tyrosine kinase receptor 3 (TrkC) is expressed in podocytes and the protein transmits signals to the podocyte actin cytoskeleton.MethodsNephron-specific TrkC knockout (TrkC-KO) and nephron-specific TrkC-overexpressing (TrkC-OE) mice were generated to dissect the role of TrkC in nephron development and maintenance.ResultsBoth TrkC-KO and TrkC-OE mice exhibited enlarged glomeruli, mesangial proliferation, basement membrane thickening, albuminuria, podocyte loss, and aspects of FSGS during aging. Igf1 receptor (Igf1R)–associated gene expression was dysregulated in TrkC-KO mouse glomeruli. Phosphoproteins associated with insulin, erb-b2 receptor tyrosine kinase (Erbb), and Toll-like receptor signaling were enriched in lysates of podocytes treated with the TrkC ligand neurotrophin-3 (Nt-3). Activation of TrkC by Nt-3 resulted in phosphorylation of the Igf1R on activating tyrosine residues in podocytes. Igf1R phosphorylation was increased in TrkC-OE mouse kidneys while it was decreased in TrkC-KO kidneys. Furthermore, TrkC expression was elevated in glomerular tissue of patients with diabetic kidney disease compared with control glomerular tissue.ConclusionsOur results show that TrkC is essential for maintaining glomerular integrity. Furthermore, TrkC modulates Igf-related signaling in podocytes. 相似文献