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排序方式: 共有129条查询结果,搜索用时 468 毫秒
1.
The effect of thrombopoietin on the proliferation and differentiation of murine hematopoietic stem cells 总被引:15,自引:16,他引:15
Sitnicka E; Lin N; Priestley GV; Fox N; Broudy VC; Wolf NS; Kaushansky K 《Blood》1996,87(12):4998-5005
In this study, we explored whether thrombopoietin (Tpo) has a direct in vitro effect on the proliferation and differentiation of long-term repopulating hematopoietic stem cells (LTR-HSC). We previously reported a cell separation method that uses the fluorescence-activated cell sorter selection of low Hoescht 33342/low Rhodamine 123 (low Ho/low Rh) fluorescence cell fractions that are highly enriched for LTR-HSC and can reconstitute lethally irradiated recipients with fewer than 20 cells. Low Ho/low Rh cells clone with high proliferative potential in vitro in the presence of stem cell factor (SCF) + interleukin-3 (IL-3) + IL-6 (90% to 100% HPP-CFC). Tpo alone did not induce proliferation of these low Ho/low Rh cells. However, in combination with SCF or IL-3, Tpo had several synergistic effects on cell proliferation. When Tpo was added to single growth factors (either SCF or IL-3 or the combination of both), the time required for the first cell division of low Ho/low Rh cells was significantly shortened and their cloning efficiency increased substantially. Moreover, the subsequent clonal expansion at the early time points of culture was significantly augmented by Tpo. Low Ho/low Rh cells, when assayed in agar directly after sorting, did not form megakaryocyte colonies in any growth condition tested. Several days of culture in the presence of multiple cytokines were required to obtain colony-forming units-megakaryocyte (CFU-Mk). In contrast, more differentiated, low Ho/high Rh cells, previously shown to contain short- term repopulating hematopoietic stem cells (STR-HSC), were able to form megakaryocyte colonies in agar when cultured in Tpo alone directly after sorting. These data establish that Tpo acts directly on primitive hematopoietic stem cells selected using the Ho/Rh method, but this effect is dependent on the presence of pluripotent cytokines. These cells subsequently differentiate into CFU-Mk, which are capable of responding to Tpo alone. Together with the results of previous reports of its effects on erythroid progenitors, these results suggest that the effects of Tpo on hematopoiesis are greater than initially anticipated. 相似文献
2.
GV Ramesh Prasad Marcel Ruzicka Kevin D Burns Sheldon W Tobe Marcel Lebel 《The Canadian journal of cardiology》2009,25(5):309-314
For the first time, the Canadian Hypertension Education Program has studied the evidence supporting blood pressure control in people requiring renal replacement therapy for end-stage kidney disease, including those on dialysis and with renal transplants. According to the Canadian Organ Replacement Registry’s 2008 annual report, there were an estimated 33,832 people with end-stage renal disease in Canada at the end of 2006, an increase of 69.7% since 1997. Of these, 20,465 were on dialysis and 13,367 were living with a functioning kidney transplant. Thus, it is becoming more likely that primary care practitioners will be helping to care for these complex patients. With the lack of large controlled clinical trials, the consensus recommendation based on interpretation of the existing literature is that blood pressure should be lowered to below 140/90 mmHg in hypertensive patients on renal replacement therapy and to below 130/80 mmHg for renal transplant patients with diabetes or chronic kidney disease. 相似文献
3.
AE Scaramuzza A De Palma C Mameli D Spiri L Santoro GV Zuccotti 《Acta paediatrica (Oslo, Norway : 1992)》2010,99(8):1237-1241
Aim: The aim of the student is to assess whether adolescents with type 1 diabetes mellitus (T1DM) in Italy differ from their healthy peers in regard to risky behaviour. Methods: Data were collected from 215 patients, aged 14 ± 2 years with a mean disease duration of 7 ± 5 years. The control group was comprised of 464 healthy adolescents recruited among high school students. Each patient completed an anonymous confidential questionnaire to determine the prevalence of sexual behaviour, alcohol and tobacco consumption, illicit drug use, and, among patients with diabetes and frequency of mismanagement related to diabetes care. Results: Compared with controls, subjects with diabetes showed a similar rate of sexual intercourse among males and lower rates among females (34.8% vs 35.5%, p NS and 29.4% vs 41.4%, p < 0.05, respectively). Males in the diabetes group reported a higher rate of tobacco use, whereas females showed similar or higher rates of use for every illicit drug studied. Among patients with diabetes, those who are engaged in risky behaviour showed a higher rate of treatment mismanagement (76% vs 34%, p < 0.01). Conclusion: Adolescents with T1DM are as likely as their healthy peers to engage in risky behaviour, indicating the potential benefit of anticipatory guidance concerning glycaemic control and increased risk of acute and chronic complications. 相似文献
4.
GV Coppa P Pierani L Zampini I Carloni A Carlucci O Gabrielli 《Acta paediatrica (Oslo, Norway : 1992)》1999,88(S430):89-94
Twenty-one oligosaccharides of human milk were quantified by high-performance anion-exchange chromatography. Milk samples were collected from 18 mothers during the first 3 mo of lactation. The data show that the highest amount of all oligosaccharides is present at day 4 postpartum (20 g l−1 ) and then decreases by about 20% at day 30 of lactation. The protective role played by these substances against different infectious agents, in different organs and systems of the breastfed baby, is emphasized. 相似文献
5.
The coeliac iceberg in Italy. A multicentre antigliadin antibodies screening for coeliac disease in school-age subjects 总被引:8,自引:0,他引:8
C Catassi E Fabiani IM Rätsch GV Coppa PL Giorgi R Pierdomenico S Alessandrini G Iwanejko R Domenici E Mei A Miano M Marani G Bottaro M Spina M Dotti A Montanelli M Barbato F Viola R Lazzari M Vallini G Guariso M Plebani F Cataldo G Traverso C Ughi G Chiaravalloti M Baldassarre P Scarcella F Bascietto L Ceglie A Valenti P Paolucci M Caradonna E Bravi A Ventura 《Acta paediatrica (Oslo, Norway : 1992)》1996,85(S412):29-35
Background : Recent studies suggest that coeliac disease (CD) is one of the commonest, life-long disorders in Italy. The aims of this multicentre work were: (a) to establish the prevalence of CD on a nationwide basis; and (b) to characterize the CD clinical spectrum in Italy. Patients and methods : Fifteen centres screened 17201 students aged 6–15 years (68.6% of the eligible population) by the combined determination of serum IgG- and IgA-antigliadin antibody (AGA) test; 1289 (7.5%) were IgG and/or IgA-AGA positive and were recalled for the second-level investigation; 111 of them met the criteria for the intestinal biopsy: IgA-AGA positivity and/or AEA positivity or IgG-AGA positivity plus serum IgA deficiency. Results : Intestinal biopsy was performed on 98 of the 111 subjects. CD was diagnosed in 82 subjects (75 biopsy proven, 7 not biopsied but with associated AGA and AEA positivity). Most of the screening-detected coeliac patients showed low-grade intensity illness often associated with decreased psychophysical well-being. There were two AEA negative cases with associated CD and IgA deficiency. The prevalence of undiagnosed CD was 4.77 × 1000 (95% CI 3.79–5.91), 1 in 210 subjects. The overall prevalence of CD, including known CD cases, was 5.44 × 1000 (95% CI 4.57–6.44), 1 in 184 subjects. The ratio of known to undiagnosed CD cases was 1 in 7. Conclusions : These findings confirm that, in Italy, CD is one of the most common chronic disorders showing a wide and heterogeneous clinical spectrum. Most CD cases remain undiagnosed unless actively searched. 相似文献
6.
Laberko LA Kuznetsov NA Aronov LS Rodoman GV Brontveĭn AT Semenova TV Fomin VV 《Khirurgiia》2004,(9):25-28
Efficacy of enteral ozone lavage in combined treatment of patients with general peritonitis is analyzed. It is demonstrated that intestinal lavage with ozone-enriched physiological salt solution through nasointestinal probe in postoperative period eliminates effectively bacterial contamination of the intestine, decreases toxicity of enteral contents and leads to significant improvement of functional characteristics of the small intestine and results of treatment. 相似文献
7.
8.
G. V. Rodoman T. I. Shalaeva G. E. Dobretsov A. L. Korotaev 《Bulletin of experimental biology and medicine》1999,128(6):1232-1234
The concentration of albumin and the state of its binding centers in the serum and abdominal exudate from patients with acute
pancreatitis were evaluated using a fluorescent technique. The total albumin concentration was below normal by 33%. The ratio
of effective to total concentration also decreased attesting to conformational changes in the albumin molecule. Albumin molecules
are released into exudate in its native form, but sometimes albumin in the exudate is dramatically changed probably due to
bursa omentalis seal failure and its concentration sharply decreased probably due to proteolytic degradation.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 12, pp. 660–662, December, 1999 相似文献
9.
Paltiel HJ; O'Gorman AM; Meagher-Villemure K; Rosenblatt B; Silver K; Watters GV 《Radiology》1987,162(1):115
10.
Prevalence and characteristics of brittle diabetes in Britain 总被引:3,自引:0,他引:3
Gill GV; Lucas S; Kent LA 《QJM : monthly journal of the Association of Physicians》1996,89(11):839-843
We investigated the prevalence and characteristics of 'brittle diabetes',
defined as insulin-dependent diabetes mellitus associated with glycaemic
instability of any type, leading to life disruption with recurrent and/or
prolonged hospitalizations. A questionnaire was sent to all physicians and
paediatricians running diabetic clinics in the UK, from lists held at the
British Diabetic Association. A total of 414 brittle patients were reported
(72% questionnaire return). Most were young (mean age +/- SD was 26 +/- 15
years), though there was a small peak at ages 60-70 years. There was an
excess of females (66%) and overall clinic prevalence was 1.2 per 1000
diabetic patients and 2.9 per 1000 insulin-treated diabetic patients. On
average, there was 1.0 brittle patient per diabetic clinic. The most common
form of brittleness was recurrent ketoacidosis (59%), with 17% having
predominant hypoglycaemia, and 24% mixed instability. Female excess was
highest and mean age lowest in the recurrent ketoacidosis group, whilst the
reverse was true for those with recurrent hypoglycaemia. Causes of
brittleness were offered by 58% of consultants, and most (93%) considered
various psychosocial problems as likely underlying factors. We conclude
that brittle diabetes is a small but significant problem, currently
affecting about 1 per 1000 diabetic patients. Most, but by no means all,
are young females--often with recurrent ketoacidosis. Older age groups are
more likely to have recurrent hypoglycaemic or mixed types of brittleness.
Perceived causes of brittleness are usually psychosocial.
相似文献