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排序方式: 共有524条查询结果,搜索用时 15 毫秒
1.
Martin Juenemann Holger Nef Helge Möllmann Pratibha Singh Christian Troidl Patrick Schramm Manfred Kaps Tibo Gerriets Franz Blaes Marlene Tschernatsch 《Immunobiology》2019,224(2):220-222
Background
An association between Tako-Tsubo cardiomyopathy (TTC) and underlying malignancies has been observed, suggesting that TTC might be the consequence of paraneoplastic phenomena. This study investigates the presence of autoantibodies against cardiomyocytes as well as adrenergic (β1, β2) and muscarinic (M2) receptors in patients with TTC.Methods and results
Serum from 20 TTC patients and 20 controls with ischemic heart disease was obtained. Indirect immunofluorescence testing for intracellular autoantibodies against cardiomyocytes showed a homogenous distribution, as in both groups 9 of 20 sera displayed a characteristic binding pattern of antibodies including vascular walls and intracellular structures. Flow cytometry analysis revealed no difference between TTC and controls in the binding of autoantibodies to the surface antigens of cardiomyocyte HL-1 cells (p?=?0.569, t-test). Flow cytometry analysis of nontransfected wild type cells (p?=?0.633, t-test), M2 receptor-transfected cells (p?=?0.687, t-test), β1 receptor-transfected cells (p?=?0.444, t-test) and β2 receptor-transfected cells (p?=?0.632, t-test) showed similar results for control and TTC sera. Likewise, the binding pattern of TTC patients with a history of neoplasia compared to those without or to controls did not differ significantly (p?>?0.05, u-test).Conclusion
Findings suggest that the presumed paraneoplastic etiology of TTC cannot be attributed to the formation of these antibodies. 相似文献2.
We investigated the capacity of human interleukin (IL) 7 to induce proliferation of B cells. Purified tonsillar B cells were cultured in the presence of IL7 with Staphylococcus aureus Cowan I (SAC) or anti-μ beads as co-mitogens. IL 7 supported a dose-dependent proliferation of anti-μ-activated B cells but did not significantly support proliferation of SAC-activated B cells. When B cells were separated on Percoll gradient into small (60%-80%) and large (50%–60%) B cells and then cultured with anti-μ beads, IL7 acted on both cell populations equally well. IL7 and BCGF (low molecular weight) were synergistic in their proliferative action on anti-μ-activated B cells in a 5-day culture. On the other hand, synergistic effect of IL 7 on activated B cells was not evident in the presence of any other factor recombinant [(r)IL 1β, rIL2, rIL3, rIL4, rIL5, rIL6, recombinant tumor necrosis factor-α, recombinant lymphotoxin, recombinant granulocyte-monocyte colony-stimulating factor and recombinant interferon-γ] we tested. IL7 did not induce IgG secretion by activated B cells. 相似文献
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Ashvini L. Punde Shruti P. Shah Yogesh V. Hase Ashish D. Waghmare Pratibha S. Shinde Bharat R. Bade Habib M. Pathan Mohit Prasad Shashikant P. Patole Sandesh Jadkar 《RSC advances》2022,12(46):30157
Layered bismuth triiodide (BiI3) is a 2D material that has emerged as an ideal choice for optical sensors. Although BiI3 has been prepared using vacuum-based deposition techniques, there is a dearth of research studies on synthesizing this material using chemical route. The present work uses a facile spin coating method with varying rotation speeds (rpm) to fabricate BiI3 material thin films for photodetection applications. The structural, optical, and morphological study of BiI3 thin films prepared at 3000–6000 rpm were investigated. XRD analysis indicates formation of BiI3 films and revealed that BiI3 has a rhombohedral crystal structure. FESEM analysis showed that BiI3 films prepared at different rpm are homogeneous, dense, and free from cracks, flaws, and protrusions. In addition, films show an island-like morphology with grain boundaries having different grain sizes, micro gaps, and the evolution of the granular morphology of BiI3 particles. The UV spectroscopy and photoluminescence analysis revealed that BiI3 films strongly absorb light in the visible region of spectra with a high absorption coefficient of ∼104 cm−1, have an optical band gap of ∼1.51 eV. A photodetector was realised using fabricated BiI3 film obtained at an optimum spin speed of 4000 rpm. It showed rapid rise and decay times of 0.4 s and 0.5 s, a responsivity of ∼100 μA W−1, external quantum efficiency of 2.1 × 10−4%, and detectivity of ∼3.69 × 106 Jones at a bias voltage of 0 V. Our results point towards a new direction for layered 2D BiI3 materials for the application in self-biased photodetectors.Layered bismuth triiodide (BiI3) is a 2D material that has emerged as an ideal choice for optical sensors. 相似文献
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Maneesh Singh Pratibha Singh Dolores Vaira Mathieu Amand Souad Rahmouni Michel Moutschen 《Immunology》2014,142(4):562-572
More than a quarter of a century of research has established chronic immune activation and dysfunctional T cells as central features of chronic HIV infection and subsequent immunodeficiency. Consequently, the search for a new immunomodulatory therapy that could reduce immune activation and improve T‐cell function has been increased. However, the lack of small animal models for in vivo HIV study has hampered progress. In the current study, we have investigated a model of cord blood haematopoietic progenitor cells (CB‐HPCs) ‐transplanted humanized NOD/LtsZ‐scidIL‐2Rγnull mice in which progression of HIV infection is associated with widespread chronic immune activation and inflammation. Indeed, HIV infection in humanized NSG mice caused up‐regulation of several T‐cell immune activation markers such as CD38, HLA‐DR, CD69 and co‐receptor CCR5. T‐cell exhaustion markers PD‐1 and CTLA‐4 were found to be significantly up‐regulated on T cells. Moreover, increased plasmatic levels of lipopolysaccharide, sCD14 and interleukin‐10 were also observed in infected mice. Treatment with minocycline resulted in a significant decrease of expression of cellular and plasma immune activation markers, inhibition of HIV replication and improved T‐cell counts in HIV‐infected humanized NSG mice. The study demonstrates that minocycline could be an effective, low‐cost adjunctive treatment to regulate chronic immune activation and replication of HIV. 相似文献
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