全文获取类型
收费全文 | 1005篇 |
免费 | 83篇 |
国内免费 | 4篇 |
专业分类
儿科学 | 23篇 |
妇产科学 | 13篇 |
基础医学 | 109篇 |
口腔科学 | 20篇 |
临床医学 | 77篇 |
内科学 | 157篇 |
皮肤病学 | 22篇 |
神经病学 | 71篇 |
特种医学 | 246篇 |
外国民族医学 | 1篇 |
外科学 | 88篇 |
综合类 | 19篇 |
一般理论 | 1篇 |
预防医学 | 122篇 |
眼科学 | 22篇 |
药学 | 58篇 |
肿瘤学 | 43篇 |
出版年
2022年 | 6篇 |
2021年 | 20篇 |
2020年 | 11篇 |
2019年 | 20篇 |
2018年 | 30篇 |
2017年 | 13篇 |
2016年 | 21篇 |
2015年 | 21篇 |
2014年 | 31篇 |
2013年 | 38篇 |
2012年 | 43篇 |
2011年 | 49篇 |
2010年 | 48篇 |
2009年 | 52篇 |
2008年 | 59篇 |
2007年 | 48篇 |
2006年 | 36篇 |
2005年 | 35篇 |
2004年 | 24篇 |
2003年 | 30篇 |
2002年 | 19篇 |
2001年 | 29篇 |
2000年 | 20篇 |
1999年 | 26篇 |
1998年 | 19篇 |
1997年 | 32篇 |
1996年 | 19篇 |
1995年 | 17篇 |
1994年 | 12篇 |
1993年 | 18篇 |
1992年 | 26篇 |
1991年 | 20篇 |
1990年 | 21篇 |
1989年 | 14篇 |
1988年 | 20篇 |
1987年 | 21篇 |
1986年 | 13篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1983年 | 11篇 |
1978年 | 6篇 |
1976年 | 8篇 |
1975年 | 6篇 |
1974年 | 5篇 |
1973年 | 7篇 |
1972年 | 6篇 |
1969年 | 5篇 |
1968年 | 6篇 |
1967年 | 5篇 |
1966年 | 5篇 |
排序方式: 共有1092条查询结果,搜索用时 31 毫秒
1.
A novel surface membrane nonglycosylated acidic polypeptide (34 kDa), encoded by a structural gene on chromosome 11, has been identified using murine monoclonal antibody (MoAb) 53.6 (IgG2a). MoAb 53.6, raised against uninduced cells of a human erythroleukemia line (HEL), recognizes a surface membrane antigen that is displayed on proliferating (cell cycle phase G1, S, and M + G2 phase) human leukocytes. The expression and redistribution (i.e., patching and capping) of the p34 kDa antigen on 27 different long-term human hematopoietic cell (HHC) lines was defined by fluorescence microscopy. These lines had been established from patients with leukemia or healthy donors and included phenotypically defined populations of T cells, B cells, and myelomonocytic cells. Almost all (greater than 95%) of the leukocytes of the 27 lines reacted strongly with MoAb 53.6. The majority of the leukocytes displayed p34 kDa antigen patching (26/27 lines; patched cells, 96-100%); moreover, 20 of 27 lines exhibited p34 kDa antigen capping (capped cells, 8-96%). Presentation of the p34 kDa antigen on surface membrane ultrastructures, imaged with immunogold using an indirect antibody labeling procedure, was illustrated by scanning electron microscopy, and endocytosis of the gold-tagged antigen-antibody complex was studied by transmission electron microscopy. The HHC lines are thought to represent immortalized populations of different human leukocyte subsets that are in different stages of maturation and/or differentiation; thus these lines should prove useful as models for further characterizing this unique p34 kDa proliferation-associated antigen and for defining the mechanisms and significance of surface membrane antigen redistribution and modulation that has been associated with leukocyte activation and propagation. 相似文献
2.
Caporale CM Capasso M Lucani M Gandolfi P De Angelis MV Di Muzio A Caporale V Uncini A . 《Journal of the peripheral nervous system : JPNS》2004,9(2):114-115
Campylobacter jejuni (C. jejunj) infection is the most common antecedent in the axonal variant of Guillain‐Barré syndrome (GBS). Antibodies against nerve gangliosides found in GBS patients recognize cross‐reactive epitopes in the lipopolysaccharide (LPS) of C. jejuni. This led to the molecular mimicry hypothesis of GBS. We immunized eleven rabbits with a LPS extracted from HS:19 C. jejuni strain isolated from a patient with GBS and complete Freund's adjuvant (CFA)(group I). In a second experiment we immunized seven rabbits with LPS, CFA and keyhole limpet hemocyanin (KLH)(group II). All group I rabbits developed high titers of anti‐LPS, anti‐GM1, anti‐GD1b antibodies and lower titers of anti‐GD1a. One rabbit, 50 days after initial inoculation, showed tremor and weakness. All rabbits of group II developed high titres of antiganglioside antibodies and six animals showed weakness 59–113 days after initial inoculation. Two rabbits died. Pathology showed mild to moderate, tendentially grouped, axonal degeneration in sciatic nerves of four out of five animals. Control rabbits of group I (immunized with CFA only) did not develop antibodies, controls of group II (immunized with CFA + KLH) developed low titers of IgG anti‐GM1. None developed neurological signs or showed axonal degeneration. C. jejuni LPS is a potent B‐cell stimulator capable to induce a strong antiganglioside response in rabbits. However, to induce the neuropathy is crucial to employ KLH, a glycoprotein known to stimulate both humoral and cellular responses. This animal model reproduces the pathogenetic process hypothesized in axonal GBS with antiganglioside antibodies post C. jejuni infection. 相似文献
3.
Peder E Z Larson Steven M Conolly John M Pauly Dwight G Nishimura 《Magnetic resonance in medicine》2007,58(5):952-961
Ultrashort echo time (UTE) imaging is a technique that can visualize tissues with sub-millisecond T(2) values that have little or no signal in conventional MRI techniques. The short-T(2) tissues, which include tendons, menisci, calcifications, and cortical bone, are often obscured by long-T(2) tissues. This paper introduces a new method of long-T(2) component suppression based on adiabatic inversion pulses that significantly improves the contrast of short-T(2) tissues. Narrow bandwidth inversion pulses are used to selectively invert only long-T(2) components. These components are then suppressed by combining images prepared with and without inversion pulses. Fat suppression can be incorporated by combining images with the pulses applied on the fat and water resonances. Scaling factors must be used in the combination to compensate for relaxation during the preparation pulses. The suppression is insensitive to RF inhomogeneities because it uses adiabatic inversion pulses. Simulations and phantom experiments demonstrate the adiabatic pulse contrast and how the scaling factors are chosen. In vivo 2D UTE images in the ankle and lower leg show excellent, robust long-T(2) suppression for visualization of cortical bone and tendons. 相似文献
4.
5.
6.
7.
William E. Gillies FRACS FRACO FRCS Anne MV Brooks MD PhD FRACS FRACO FRACP 《Clinical & experimental ophthalmology》1996,24(3):169-187
Background: We have investigated the vascular perfusion of a wide variety of conditions of the anterior segment using fluorescein angiography.
Methods: The conditions were classified and findings reported according to the system set out below. Patients underwent full ocular examination. Fluorescein angiography of the anterior segment was carried out when indicated to investigate iris atrophy and neovascularisation. Specular microscopy of the corneal endothelium was used to detect changes in this tissue.
Results: The hypoperfusion was variable in degree and accompanied by varying degrees of iris hypoplasia and atrophy with neovascularisation. The degree of neovascularisation depended upon its rapidity of development, the pre-existing state of vascular perfusion and the underlying pathological condition.
Conclusions: Hypoperfusion with resultant ischaemia and neovascularisation is common in conditions of the anterior segment. An understanding of the changes is valuable in treating many conditions affecting the anterior segment. The changes observed may also occur elsewhere in the physical system and may be a significant part of the ageing process, either as scattered, disparate processes or as part of a general disease process. 相似文献
Methods: The conditions were classified and findings reported according to the system set out below. Patients underwent full ocular examination. Fluorescein angiography of the anterior segment was carried out when indicated to investigate iris atrophy and neovascularisation. Specular microscopy of the corneal endothelium was used to detect changes in this tissue.
Results: The hypoperfusion was variable in degree and accompanied by varying degrees of iris hypoplasia and atrophy with neovascularisation. The degree of neovascularisation depended upon its rapidity of development, the pre-existing state of vascular perfusion and the underlying pathological condition.
Conclusions: Hypoperfusion with resultant ischaemia and neovascularisation is common in conditions of the anterior segment. An understanding of the changes is valuable in treating many conditions affecting the anterior segment. The changes observed may also occur elsewhere in the physical system and may be a significant part of the ageing process, either as scattered, disparate processes or as part of a general disease process. 相似文献
8.
Postoperative radiation therapy in the management of lung cancer 总被引:1,自引:0,他引:1
Postoperative radiation therapy for lung cancer is still controversial. In a 9-year period, 69 patients with non-oat-cell carcinoma of the lung (16% stage I, 26% stage II, and 58% stage III) received such therapy. The radiation dose was less than 5,000 cGy in 42 patients, 5,000-5,900 cGy in 16, and 6,000 cGy or more in 11; follow-up ranged from 24 to 64 months. Actuarial survival at 2 and 4 years was 50% and 16%, respectively, for squamous cell carcinoma, and 40% and 26% for adenocarcinoma. The 5-year survival for stages I, II, and III cancer was 29%, 17%, and 19%, respectively. Histologic findings and type of surgery did not affect survival, but the radiation dose apparently did. The 3-year survival for patients who received less than 6,000 cGy was 35%, compared with 73% for patients who received higher doses. In eight patients, treatment failed within the irradiated volume: all had received doses of less than 6,000 cGy, and the volume in three was judged to be inadequate. 相似文献
9.
10.
L E Scheving W H Harrison P Gordon J E Pauly 《The American journal of physiology》1968,214(1):166-173