Iliopsoas Abscess Due to Brenner Tumor Malignancy: A Case Report

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种植受体血管内皮细胞在异种血管移植中的实验研究

目的　探讨种植受体血管内皮细胞在抗异种血管移植超急排斥反应 (HAR)方面的意义。方法　选用豚鼠→大鼠移植模型 ,先将分离、培养的大鼠动脉内皮细胞种植于去内皮的豚鼠动脉内壁 ,再将此豚鼠动脉植入大鼠体内 ,观察动脉血栓形成时间 ,并采用免疫组化技术检测了 Ig M、C3在移植后豚鼠动脉壁上沉积的情况。结果　已种植大鼠内皮细胞的豚鼠动脉血栓形成时间 (2 0 .3± 4.42 h)较植入大鼠体内的正常豚鼠动脉 (0 .35± 0 .2 84h)以及单纯去内皮豚鼠动脉血栓形成时间 (0 .16 5± 0 .0 77h)显著延长 (P<0 .0 0 1)。免疫组化结果显示 ,植入大鼠体内的正常豚鼠内皮细胞表面有 Ig M和 C3沉积 ,而预先种植大鼠内皮细胞的豚鼠动脉内壁无 Ig M和 C3沉积。结论　在异种供体血管壁上预先种植受者血管内皮细胞 ,可延长移植的异种供体血管通畅时间 ,该技术可能在抗异种血管移植 HAR方面具有重要意义     

妇产科髂内动脉结扎术21例分析

髂内动脉结扎术是控制妇产科手术中大出血的有效措施。本文收集此类患者２１例，行双侧髂内动脉结扎１５例，单侧６例，均达到了满意的止血效果。笔者介绍了馆内动脉结扎术的方法和临床应用，以及预防髂内静脉损伤和误扎髂多外动脉的具体措施。着重提出髂内动脉结扎后再局部止血才能完全控制盆腔大出血。     

医院后勤服务社会化实践中的几点思考

就医院后勤服务社会化的实际操作中的几个问题进行了论述,指出实行后勤社会化服务,必须有相应的外部环境;后勤部门实行企业化管理,必须有相配套的政策作保障;实施商品化服务,必须有对等的经济意识;加大后勤服务化的力度,必须有果敢超前的决策意识。     

Effects of Electro-Acupuncture on Immune Function After Chemotherapy in 28 Cases

PURPOSE: To observe the effects of electroacupuncture therapy on T cells and activity of NK cell in the patient of Chemotherapy. METHOD: Electro-acupuncture therapy was simultaneously applied during chemotherapy, T cells and activity of NK cell of patients were determined before electroacupuncture treatment (before chemotherapy) and after 4-course electro-acupuncture treatments. RESULTS: Before chemotherapy, CD3 was low within the normal range, CD4 was much lower than the normal range, and CD8, CD4/CD8 and activity of NK cell were within the normal range. After one month of chemotherapy combined with electro-acupuncture, no decline of all the indices was found (P > 0.05). CONCLUSION: Electro-acupuncture can really increase the immune function of patients of chemotherapy.     

一期动静脉转流与并加压破坏深静脉瓣对比的实验研究

目的:改进一期动静脉转流术的疗效。方法:10只狗双后肢,随机选择一侧后肢行一期动静脉转流,另一侧行一期动静脉转流加水压破坏深静脉瓣。术后30天通过动脉造影进行对比研究。结果:后者能更有效地加速远段深静脉瓣的破坏,使动脉血逆行更远,同时形成更为丰富的侧枝循环,而不会增加肢体肿胀和深静脉血栓的机会。结论:一期动静脉转流加水压破坏深静脉瓣可能是治疗严重肢体缺血更为有效的方法。     

Intracellular Abeta is increased by okadaic acid exposure in transfected neuronal and non-neuronal cell lines.

Intracellular Abeta was examined in both a neuronal cell line (B103) expressing human APP with Swedish mutation and a non-neuronal cell line (Chinese hamster ovary, CHO) expressing wild human APP. Exposure of the APP695sw-transfected B103 cells to okadaic acid for 3 h, Abeta immunostaining was enhanced, as demonstrated by two independent anti-Abeta antibodies. The confocal microscopic study revealed that the immunoreactivity of Abeta was mainly colocalized with a Golgi marker and partially with an ER marker. Quantitative analyses, using Abeta sandwich ELISA, showed significantly increased intracellular Abeta. False positive detection of Abeta by antibody cross-reaction with APP was ruled out by extracting the fraction with formic acid and making it alkaline before subjecting it to ELISA. This procedure resulted in a fraction that contained little APP. Using CHO cells, OA treatment was also shown to be effective in increasing Abeta, as demonstrated by Western blot. The increased full-length APP and decreased APPC99 were also observed. This is the first study to demonstrate that OA treatment significantly increases intracellular Abeta.     

E. coli-expressed recombinant norovirus capsid proteins maintain authentic antigenicity and receptor binding capability

The baculovirus expression system has been widely used to produce the capsid proteins of Norovirus (NV) and the proteins form virus-like particles (VLPs) that are useful in many studies, such as immunology, diagnosis, and host-receptor interaction. We report here the application of the E. coli expression system in the production of recombinant NV capsid proteins. In a direct comparison of a previous well-characterized NV strain (VA387), we have demonstrated that the E. coli-expressed capsid proteins maintain the same antigenicity and receptor binding specificity as that of the baculovirus-expressed capsid, although the E. coli-expressed VA387 proteins did not form VLPs. Using the E. coli-expression system, we characterized the receptor-binding patterns of three additional NV strains (OIF1998, Parris Island and VA115), in which OIF1998 binds to HBGA of nonsecretors but did not bind or binds weakly to the HBGA of secretors, as seen in strain VA207. Parris Island binds to HBGA of types A and B but not type O secretors and nonsecretors. VA115 did not show specific binding to any A, B, O secretor nor nonsecretor, which is also observed when the capsid protein of this strain was expressed in baculovirus. Our data indicate that VLP formation is not required for receptor binding, and that the bacteria expression system offers a simple alternative for large production of NV capsid protein for various research purposes, particularly for strains generating low yields in the insect cells.     

成人特发性局灶节段性肾小球硬化病248例临床病理分析

目的 重新评价肾活检标本中特发性局灶节段性肾小球硬化病（FSGS）的发生率、主要临床及病理特点。方法 收集1994年7月～2000年7月间248例特发性FSGS的患者,观察肾组织的光镜、 荧光及电镜的特点,根据肾小球病变将特发性FSGS分为3种亚型。结果 248例成人特发性局灶节段肾小球硬化占同期肾活检的12.4%,占成年人原发性肾小球疾病的19.0%,以18～30岁为发病高峰年龄（约占44.4%）。临床表现为不同程度的蛋白尿,以肾病综合征为表现者143例（占57.7%)。FSGS的3种病理亚型中,经典型FSGS仍占绝大多数（91.5%),尖端型为4.4%,塌陷型为4.1%,肾活检时,约1/3以上为肾功能不全（约占35.1%）。13例尿毒症中76.9%病理亚型为塌陷型。结论 局灶节段性肾小球硬化病约占原发性肾小球病1/5,FSGS患者肾活检时,除表现为蛋白尿、肾病综合征外,肾功能不全也较常见。塌陷型FSGS常表现为进行性的不可逆的肾功能衰竭,其预后差。     

后路椎体次全切治疗胸腰段椎体成形术椎再骨折的疗效分析

目的探讨经后路椎体次全切治疗胸腰段椎体成形术椎再骨折的疗效。方法回顾性分析2017年6月至2019年10月河南省人民医院脊柱外科收治的28例胸腰段椎体成形术椎再骨折患者资料。男7例,女21例;年龄61~76岁,平均62.4岁;骨折椎体:T115例,T129例,L111例,L23例;既往手术方法:经皮椎体成形术治疗17例,经皮椎体后凸成形术治疗11例。手术方法:后路切除棘突、椎板,经侧方切除关节突、横突,部分切除椎体、骨水泥及上下椎间盘,神经减压,椎体间人工椎体固定,后路钉棒矫形固定。记录手术时间及术中出血量,比较患者术前和末次随访时后凸cobb角评价矫形效果;比较患者术前和末次随访时疼痛视觉模拟评分(VAS)、日本骨科协会(JOA)评分评价脊柱功能改善情况。结果本组患者手术时间平均为182.1 min,术中出血量平均为996.2 mL。28例患者术后随访8~29个月(平均19.8个月),无明显神经功能损伤及其他严重并发症发生。cobb角由术前的41.3°±10.3°改善为末次随访时的6.4°±2.5°,VAS评分由术前的(7.3±1.8)分改善为末次随访时的(2.5±1.0)分,JOA评分由术前的(8.4±2.3)分改善为末次随访时的(21.3±2.5)分,以上比较差异均有统计学意义(P<0.05)。结论经后路椎体次全切治疗胸腰段椎体成形术椎再骨折,可一期进行骨水泥清除、椎管减压、椎间植骨融合,重建脊柱稳定性,有很好的疗效。