全文获取类型
收费全文 | 1060篇 |
免费 | 30篇 |
国内免费 | 65篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 65篇 |
妇产科学 | 11篇 |
基础医学 | 98篇 |
口腔科学 | 36篇 |
临床医学 | 140篇 |
内科学 | 217篇 |
皮肤病学 | 25篇 |
神经病学 | 57篇 |
特种医学 | 245篇 |
外科学 | 106篇 |
综合类 | 29篇 |
预防医学 | 22篇 |
眼科学 | 20篇 |
药学 | 47篇 |
肿瘤学 | 35篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 8篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 16篇 |
2014年 | 13篇 |
2013年 | 27篇 |
2012年 | 21篇 |
2011年 | 17篇 |
2010年 | 32篇 |
2009年 | 44篇 |
2008年 | 22篇 |
2007年 | 67篇 |
2006年 | 31篇 |
2005年 | 31篇 |
2004年 | 22篇 |
2003年 | 16篇 |
2002年 | 22篇 |
2001年 | 22篇 |
2000年 | 18篇 |
1999年 | 23篇 |
1998年 | 65篇 |
1997年 | 71篇 |
1996年 | 71篇 |
1995年 | 60篇 |
1994年 | 43篇 |
1993年 | 52篇 |
1992年 | 11篇 |
1991年 | 15篇 |
1990年 | 19篇 |
1989年 | 40篇 |
1988年 | 22篇 |
1987年 | 38篇 |
1986年 | 13篇 |
1985年 | 24篇 |
1984年 | 15篇 |
1983年 | 23篇 |
1982年 | 19篇 |
1981年 | 17篇 |
1980年 | 20篇 |
1978年 | 5篇 |
1977年 | 9篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1966年 | 2篇 |
1933年 | 1篇 |
排序方式: 共有1155条查询结果,搜索用时 15 毫秒
1.
2.
Kaufmann SJ; Sharif K; Sharma V; McVerry BA 《Human reproduction (Oxford, England)》1998,13(2):498-499
The patient was diagnosed in childhood as having severe congenital
neutropenia and had recurrent admissions with severe infections. In 1987,
prior to getting married, she was sterilized. She continued to require i.v.
antibiotics when she contracted a severe infection. On one occasion, she
was treated with growth colony stimulating factor (G- CSF). Her increased
neutrophil count was sustained following this treatment. In June 1993, she
wished to start a family and underwent in- vitro fertilization (IVF)
treatment. G-CSF was given prior to oocyte retrieval. She conceived on her
first cycle and an ultrasound scan revealed a singleton pregnancy.
Throughout the course of the pregnancy, her white cell count was monitored
closely and remained at <1.0x10(9)/l. The pregnancy progressed
uneventfully and at 37 weeks gestation she was admitted for G-CSF
injections. At 38 weeks she was delivered of a boy weighing 3350 g, by
elective Caesarean section. His white cell count was normal. This is the
first case of G-CSF being used before conception and during pregnancy in a
patient with congenital neutropenia. It shows that advances in cytokine
therapy and close interdisciplinary liaison can lead to a successful
outcome and help patients, who would otherwise remain childless, to achieve
a family.
相似文献
3.
Silber SJ; Nagy Z; Devroey P; Tournaye H; Van Steirteghem AC 《Human reproduction (Oxford, England)》1997,12(11):2422-2428
The aim of the study was to determine whether a prior diagnostic testicle
biopsy can predict success or failure of testicular sperm extraction (TESE)
with intracytoplasmic sperm injection (ICSI) in patients with
non-obstructive azoospermia caused by testicular failure, and what is the
minimum threshold of sperm production in the testis which must be surpassed
for spermatozoa to reach the ejaculate. Forty- five patients with
non-obstructive azoospermia caused by testicular failure underwent
diagnostic testicle biopsy prior to a planned future TESE-ICSI procedure.
The diagnostic testicle biopsy was analysed quantitatively, and correlated
with the quantitative findings of spermatogenesis in patients with normal
spermatogenesis, as well as with the results of subsequent attempts at
TESE-ICSI. Men with non- obstructive azoospermia caused by germinal failure
had a mean of 0-6 mature spermatids/seminiferous tubule seen on a
diagnostic testicle biopsy, compared to 17-35 mature spermatids/tubule in
men with normal spermatogenesis and obstructive azoospermia. These findings
were the same for all types of testicular failure whether Sertoli cell
only, maturation arrest, cryptorchidism, or post-chemotherapy azoospermia.
Twenty-two of 26 men with mature spermatids found in the prior testis
biopsy had successful retrieval of spermatozoa for ICSI, 12 of their
partners became pregnant, and are either ongoing or delivered. The study
suggests that 4-6 mature spermatids/tubule must be present in the testis
biopsy for any spermatozoa to reach the ejaculate. More than half of
azoospermic patients with germinal failure have minute foci of
spermatogenesis which are insufficient to produce spermatozoa in the
ejaculate. Prior diagnostic testicle biopsy analysed quantitatively (for
the presence of mature spermatids) can predict subsequent success or
failure with TESE-ICSI. Incomplete testicular failure may involve a sparse
multi-focal distribution of spermatogenesis throughout the entire testicle,
rather than a regional distribution. Therefore, it is possible that massive
testicular sampling from many different regions of the testes may not be
necessary for successful TESE-ICSI.
相似文献
4.
β-Lactoglobulin was isolated from infant formulae that were ultra high temperature (UHT) -treated, sterilized or spray-dried. The effect of the isolated β-lactoglobulin on SfaII-fimbriae-mediated adhesion of Escherichia coli to human ileostomy glycoproteins was studied in vitro. β-Lactoglobulin isolated from sterilized formulae was found to perform significantly less well than preparations from spray-dried formulae (p = 0:05). Great heterogeneity was observed in the adhesion inhibitory capacity of β-lactoglobulin isolated from UHT-treated formulae. Therefore, no significant difference was observed between UHT-treated and sterilized formulae or spray-dried formulae (p < 0:10). It can be hypothesized that β-lactoglobulin from spray-dried and some UHT-treated infant formulae may affect the colonization of mucous membranes by E. coli strains causing neonatal septicaemia and meningitis. 相似文献
5.
6.
7.
Cajal-Retzius cells are among the first neurons appearing during corticogenesis and play an important role in the establishment of cortical lamination. To characterize the hyperpolarization-activated inward current (I(h)) and to investigate whether I(h) contributes to the relatively positive resting membrane potential (RMP) of these cells, we analyzed the properties of I(h) in visually identified Cajal-Retzius cells in cortical slices from neonatal rats using the whole cell patch-clamp technique. Membrane hyperpolarization to -90 mV activated a prominent inward current that was inhibited by 1 mM Cs(+) and was insensitive to 1 mM Ba(2+). The activation time constant for I(h) was strongly voltage dependent. In Na(+)-free solution, I(h) was reduced, indicating a contribution of Na(+). An analysis of the tail currents revealed a reversal potential of -45.2 mV, corresponding to a permeability coefficient (pNa(+)/pK(+)) of 0. 13. While an increase in the extracellular K(+) concentration ([K(+)](e)) enhances I(h), it was reduced by a [K(+)](e) decrease. This [K(+)](e) dependence could not be explained by an effect on the electromotive force on K(+) but suggested an additional extracellular binding site for K(+) with an apparent dissociation constant of 7.2 mM. Complete Cl(-) substitution by Br(-), I(-), or NO(3)(-) had no significant effect on I(h), whereas a complete Cl(-) substitution by the organic compounds methylsulfate, isethionate, or gluconate reduced I(h) by approximately 40%. The I(h) reduction observed in gluconate could be abolished by the addition of Cl(-). The analysis of the [Cl(-)](e) dependence of I(h) revealed a dissociation constant of 9.8 mM and a Hill-coefficient of 2.5, while the assumption of a gluconate-dependent I(h) reduction required an unreasonably high Hill-coefficient >20. An internal perfusion with the lidocaine derivative lidocaine N-ethyl bromide blocks I(h) within 1 min after establishment of the whole cell configuration. An inhibition of I(h) by 1 mM Cs(+) was without an effect on RMP, action potential amplitude, threshold, width, or afterhyperpolarization. We conclude from these results that Cajal-Retzius cells express a prominent I(h) with characteristic properties that does not contribute to the RMP. 相似文献
8.
Preimplantation genetic diagnosis principles and ethics 总被引:4,自引:0,他引:4
9.
Life expectancy in British Marfan syndrome populations 总被引:2,自引:0,他引:2
JR Gray AB Bridges RR West L. McLeish AG Stuart JCS Dean MEM Porteous M. Boxer SJ Davies 《Clinical genetics》1998,54(2):124-128
A total of 206 patients with Marfan syndrome were ascertained throughout genetic clinics in Wales and Scotland during the period 1970–1990. There were 45 deaths representing 22% of the cohort. Mean age at death was 45.3 ± 16.5 years. 50% median cumulative survival in the total cohort (n = 206) was 53 years for males and 72 years for females. Multivariate analysis confirmed severity as the best independent indicator of survival. These findings and survival curves will assist in the counselling of British families and individuals with Marfan syndrome. 相似文献
10.
Frataxin is reduced in Friedreich ataxia patients and is associated with mitochondrial membranes 总被引:17,自引:8,他引:17
Campuzano V; Montermini L; Lutz Y; Cova L; Hindelang C; Jiralerspong S; Trottier Y; Kish SJ; Faucheux B; Trouillas P; Authier FJ; Durr A; Mandel JL; Vescovi A; Pandolfo M; Koenig M 《Human molecular genetics》1997,6(11):1771-1780
Friedreich ataxia is a progressive neurodegenerative disorder caused by
loss of function mutations in the frataxin gene. In order to unravel
frataxin function we developed monoclonal antibodies raised against
different regions of the protein. These antibodies detect a processed 18
kDa protein in various human and mouse tissues and cell lines that is
severely reduced in Friedreich ataxia patients. By immunocytofluorescence
and immunocytoelectron microscopy we show that frataxin is located in
mitochondria, associated with the mitochondrial membranes and crests.
Analysis of cellular localization of various truncated forms of frataxin
expressed in cultured cells and evidence of removal of an N-terminal
epitope during protein maturation demonstrated that the mitochondrial
targetting sequence is encoded by the first 20 amino acids. Given the
shared clinical features between Friedreich ataxia, vitamin E deficiency
and some mitochondriopathies, our data suggest that a reduction in frataxin
results in oxidative damage.
相似文献