全文获取类型
收费全文 | 465篇 |
免费 | 26篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 45篇 |
妇产科学 | 9篇 |
基础医学 | 62篇 |
口腔科学 | 22篇 |
临床医学 | 35篇 |
内科学 | 84篇 |
皮肤病学 | 3篇 |
神经病学 | 20篇 |
特种医学 | 18篇 |
外国民族医学 | 1篇 |
外科学 | 82篇 |
综合类 | 3篇 |
预防医学 | 16篇 |
眼科学 | 10篇 |
药学 | 32篇 |
中国医学 | 3篇 |
肿瘤学 | 45篇 |
出版年
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 22篇 |
2020年 | 9篇 |
2019年 | 17篇 |
2018年 | 22篇 |
2017年 | 9篇 |
2016年 | 9篇 |
2015年 | 12篇 |
2014年 | 26篇 |
2013年 | 19篇 |
2012年 | 32篇 |
2011年 | 32篇 |
2010年 | 12篇 |
2009年 | 14篇 |
2008年 | 29篇 |
2007年 | 31篇 |
2006年 | 20篇 |
2005年 | 18篇 |
2004年 | 18篇 |
2003年 | 11篇 |
2002年 | 4篇 |
2001年 | 7篇 |
2000年 | 6篇 |
1999年 | 10篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1992年 | 3篇 |
1990年 | 6篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 11篇 |
1985年 | 11篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 7篇 |
1969年 | 1篇 |
1967年 | 1篇 |
1965年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有491条查询结果,搜索用时 15 毫秒
1.
2.
Examination of adult rat brain regions by specific radioimmunoassays revealed a widespread distribution of transforming growth factor-alpha (TGF-alpha), but not epidermal growth factor (EGF), the peptide that had previously been reported to be present in rodent brain. Polyadenylated RNA samples from the different regions of rat brain were analyzed by Northern blot to identify mRNA species encoding precursor proteins for EGF (preproEGF), TGF-alpha (preproTGF-alpha), and the EGF/TGF-alpha receptor. The results indicate that TGF-alpha is the most abundant ligand for the EGF/TGF-alpha receptor in most parts of the brain analyzed. Message for preproEGF was only detectable after prolonged autoradiographic exposure; levels of preproEGF mRNA were between two and three orders of magnitude lower in brain than those expressed in control tissue (kidney), and one to two orders of magnitude lower than preproTGF-alpha mRNA levels in all brain regions. These results were confirmed by analysis of mRNA by RT/PCR, and support the hypothesis that expression of preproEGF mRNA in the brain is limited to smaller discrete areas, whereas preproTGF-alpha gene expression is almost ubiquitous. 相似文献
3.
Nakashima Y Tagawa H Suzuki R Karnan S Karube K Ohshima K Muta K Nawata H Morishima Y Nakamura S Seto M 《Genes, chromosomes & cancer》2005,44(3):247-255
Natural killer (NK) cell lymphomas/leukemias are highly aggressive lymphoid malignancies, but little is known about their genomic alterations, and thus there is an urgent need for identification and analysis of NK cell lymphomas/leukemias. Recently, we developed our own array-based comparative genomic hybridization (array CGH) with an average resolution of 1.3 Mb. We performed an array CGH analysis for 27 NK-cell lymphoma/leukemia cases that were classified into two disease groups based on the World Health Organization Classification (10 aggressive NK-cell leukemia cases and 17 extranodal NK/T-cell [NK/T] lymphomas, nasal type). We identified the differences in the genomic alteration patterns of the two groups. The recurrent regions characteristic of the aggressive NK-cell leukemia group compared with those of the extranodal NK/T lymphoma, nasal-type group, were gain of 1q and loss of 7p15.1-p22.3 and 17p13.1. In particular, gain of 1q23.1-24.2 (P = 0.041) and 1q31.3-q44 (P = 0.003-0.047), and loss of 7p15.1-p22.3 (P = 0.012-0.041) and 17p13.1 (P = 0.012) occurred significantly more frequently in the former than in the latter group. Recurrent regions characteristic of the extranodal NK/T lymphoma, nasal-type group, compared with those of the other group were gain of 2q, and loss of 6q16.1-q27, 11q22.3-q23.3, 5p14.1-p14.3, 5q34-q35.3, 1p36.23-p36.33, 2p16.1-p16.3, 4q12, and 4q31.3-q32.1. Our results can be expected to provide further insights into the genetic basis of lymphomagenesis and the clinicopathologic features of NK-cell lymphomas/leukemias. 相似文献
4.
5.
Lakshmanan V Rhee KY Wang W Yu Y Khafizov K Fiser A Wu P Ndir O Mboup S Ndiaye D Daily JP 《The Journal of infectious diseases》2012,206(2):238-248
Metabolomics offers a powerful means to investigate human malaria parasite biology and host-parasite interactions at the biochemical level, and to discover novel therapeutic targets and biomarkers of infection. Here, we used an approach based on liquid chromatography and mass spectrometry to perform an untargeted metabolomic analysis of metabolite extracts from Plasmodium falciparum-infected and uninfected patient plasma samples, and from an enriched population of in vitro cultured P. falciparum-infected and uninfected erythrocytes. Statistical modeling robustly segregated infected and uninfected samples based on metabolite species with significantly different abundances. Metabolites of the α-linolenic acid (ALA) pathway, known to exist in plants but not known to exist in P. falciparum until now, were enriched in infected plasma and erythrocyte samples. In vitro labeling with (13)C-ALA showed evidence of plant-like ALA pathway intermediates in P. falciparum. Ortholog searches using ALA pathway enzyme sequences from 8 available plant genomes identified several genes in the P. falciparum genome that were predicted to potentially encode the corresponding enzymes in the hitherto unannotated P. falciparum pathway. These data suggest that our approach can be used to discover novel facets of host/malaria parasite biology in a high-throughput manner. 相似文献
6.
Imayavaramban Lakshmanan Sanjib Chaudhary Raghupathy Vengoji Parthasarathy Seshacharyulu Satyanarayana Rachagani Joseph Carmicheal Rahat Jahan Pranita Atri Ramakanth ChirravuriVenkata Rohitesh Gupta Saravanakumar Marimuthu Naveenkumar Perumal Sanchita Rauth Sukhwinder Kaur Kavita Mallya Lynette M. Smith Subodh M. Lele Moorthy P. Ponnusamy Mohd W. Nasser Ravi Salgia Surinder K. Batra Apar Kishor Ganti 《Molecular oncology》2021,15(7):1866
7.
8.
9.
Purpose
In vitro anticancer effect and in vivo biodistribution and biocompatibility of metformin encapsulated O-Carboxymethyl chitosan nanoparticles were evaluated for its application as pancreatic cancer therapy.Methods
In vitro studies such as cell migration assay, clonogenic assay, cell cycle analysis and qRT-PCR analysis were done in pancreatic cancer cells (MiaPaCa-2) treated with O-CMC-metformin NPs for evaluating its anticancer potential. In vivo biodistribution studies were carried out by NIR imaging of O-CMC-metformin NPs after tagging it with ICG. In vivo biocompatibility of the NPs was assessed by histopathology analysis of organs from mice administered with the NPs.Results
In vitro cell migration assay showed marginal effect of NPs on migration property of pancreatic cancer cells (MiaPaCa-2). In vitro clonogenic assay established that the O-CMC-metformin NPs reduced colony formation ability of the cancer cells. While cell cycle analysis showed that the O-CMC-metformin NPs had only minor effect on progression of cell cycle in the cancer cells. qRT-PCR analysis exhibited reduced mRNA expression of p21, vanin 1 and MMP9 in pancreatic cancer cells treated with the nanoparticles. In vivo NIR imaging study showed normal biodistribution pattern of the intravenously injected O-CMC-metformin NPs suggesting normal clearance rate of nanoparticles and no adverse toxicity to the organs.Conclusions
The biocompatible O-CMC-metformin NPs with anticancer potential and capability for normal biodistribution can be beneficial for the treatment of pancreatic cancer. 相似文献10.
Larisa Kovacevic Cortney Wolfe-Christensen Jelena Mirkovic Jessica Yih Yegappan Lakshmanan 《Pediatric nephrology (Berlin, Germany)》2014,29(7):1189-1194