氯丁二烯对小鼠血浆及脾脏淋巴细胞中cAMP和cGMP含量的影响

氯丁二烯经皮下注射给小鼠染毒,可引起血浆及脾脏淋巴细胞中cAMP含量升高和cGMP含量降低,二者含量的比值亦明显升高。结果提示,氯丁二烯可能通过改变血浆中cAMP和cGMP的含量而影响机体的生理功能及代谢;氯丁二烯所致淋巴细胞中的cAMP和cGMP含量的变化可能是引起细胞免疫和体液免疫毒性的机理之一。     

LONG TERM SUPPLEMENTATION OF SE-ENRICHED MALT FOR THE HIGH RISK POPULATION OF LUNG CANCER: INHIBITION OF ULTRAVIOLET LIGHT AND BENZOaPYRENE INDUCED UNSCHEDULED AND SYNTHESIS OF PERIPHERAL LYMPHOCYTES

Se malt cakes containing 300μg selenium were taken up daily to men from high risk area in lung cancer and the influences of Ultraviolet (UV) and Benaopyrene (BαP) induced unscheduled DNA synthesis (UDS) were determined. The Se levels in serum, hair and activity of GSH-px were increased by 89%, 67% and 178%, respectively, after Se-supplementation for half year. In the UV treatment, the ratio of UDS was decreased from the mean values of 2. 47 in the control to 1. 61 (P<0. 05) in the Se-group, In the BaP treatment, furthermore, the elevated Se levels of 78% in serum and 83% in the hair accompanied with 56% high in activity of GSH-px were followed by the Se intake for one year, while the mean value of UDS was reduced from 2. 21 in the control to 1. 47(P< 0. 05) in the group of selenium tested. The blocking effects of UV irradiation and BaP treatment induced UDS of peripheral lymphocytes were showed in the Se-supplementation.     

地质勘探作业呼吸性粉尘与尘肺的剂量-反应关系

目的研究地质勘探作业呼吸性粉尘浓度接触阈限值.方法用寿命表法分析地质勘探坑探掘进接尘工人呼吸性粉尘吸入量与尘肺发病的剂量-反应关系.结果地质勘探坑探掘进接尘工人的呼吸性粉尘接触浓度与环境中粉尘浓度的比值为1∶4.6,粉尘中游离二氧化硅含量的平均值为(48±9)%,剂量-反应关系回归方程为Y=3.2506lgX-1.0607.结论地质勘探作业呼吸性粉尘(含游离二氧化硅48%)浓度接触阈限值为0.4696mg/m3.     

胰岛素对全血血小板膜P-选择素表达影响的研究

目的探讨胰岛素对血小板膜P-选择素表达的影响。方法建议全血血小板膜P-选择素表达的流式细胞术(FCM)测定方法,观察健康青年人胰岛素在静止和活化状态下对血小板膜P-选择素表达的影响。结果胰岛素对正常人静息血小板膜P-选择素表达无明显影响;而显著抑制凝血酶、胶原活化的血小板膜P-选择素的上调表达,且该效应呈剂量和时间依赖性。结论胰岛素可抑制活化血小板膜P-选择素的表达。     

The epidemiology of lymphangioleiomyomatosis in Japan: a nationwide cross-sectional study of presenting features and prognostic factors

BACKGROUND AND OBJECTIVE: To evaluate the characteristics and prognostic factors of Japanese patients with lymphangioleiomyomatosis (LAM). METHODS: A nationwide survey to identify patients with LAM was conducted by questionnaire. Survival probability was estimated using the Kaplan-Meier method, and the prognostic factors were analysed by Cox regression. RESULTS: Data were collected on 173 patients with pulmonary LAM. The major presenting features were pneumothorax (43%) and exertional dyspnoea (37%). The survival probabilities for patients presenting with exertional dyspnoea (Group A) were 85%, 60% and 47% after 5, 10 and 15 years, respectively, and for patients presenting with pneumothorax (Group B) were 95%, 89% and 89%, respectively. Although the age at symptom onset was higher among patients in Group A than in Group B, Cox regression revealed that the presenting feature was a prognostic factor independent of age at symptom onset (Group A/B hazard ratio = 5.732, P < 0.01). In the subgroup of patients whose initial FEV(1) was >1000 mL, or FEV(1)/FVC >40%, or %DL(CO) >40%, the rate of deterioration in these tests was greater in Group A than in Group B (P < 0.01 for FEV(1), P < 0.05 for FEV(1)/FVC and %DL(CO)). CONCLUSIONS: There are two possible subgroups of LAM patients. One subgroup that presented with pneumothorax, had onset of symptoms at a younger age and a more favourable prognosis; the other presented with exertional dyspnoea, had onset of symptoms at an older age and a poorer prognosis.     

苯乙烯生物标志物的研究

目的 研究苯乙烯的生物标志物，为苯乙烯的生物监测提供理论依据。方法 采用高效液相色谱法对苯乙醇酸(MA)、苯乙醛酸(PGA)、苯乙烯巯基尿酸(MUA)进行监浏。结果 晨尿中MA、PGA、MUA和苯乙烯接触浓度间相关关系分别为y＝2．58x 70．82，y＝1．66. 37．42，y＝0．05x 0．555;班末尿中MA、PGA、MUA和苯乙烯接触浓度间相关关系分别为y＝1．85x 89．02，y=1．33x 4．32，y＝0．04x |0．68，均呈良好的相关性。结论 晨尿及班末尿中的MA、PGA、MUA测定均可作为苯乙烯的生物监测指标。     

空气中乙胺的气相色谱测定方法研究

本文建立了对空气中乙胺用硅胶管采集、稀硫酸解吸、气相色谱分析的新方法,系统地研究了样品采集、洗脱、干扰及气相色谱分离条件等技术。本法现场应用效果满意。其精密度为2.2～4.2%,检出下限为0.011μg/4μl,采样效率为99.5%,解吸效率为98%。     

纺织女工服用咖啡因对血脂、脂蛋白的影响及血清PK活性值的测定——Ⅰ.纺织女工服用咖啡因对血脂、脂蛋白的影响

测定了接触强噪声的纺织女工在饮用含咖啡因的咖啡前后血液TG,Ch,LDL-ch,和HDL-ch;以不接触噪声的女大学生作对照。结果表明,纺织女工血液TG、ch,LDL-ch显著升高,服用咖啡因后明显降低,而HDL-ch含量增多,提示咖啡因可改善接触强噪声的纺织女工的脂代谢异常。