排序方式: 共有16条查询结果,搜索用时 15 毫秒
1.
Michels Guido Horn Rudolf Helfen Andreas Hagendorff Andreas Jung Christian Hoffmann Beatrice Jaspers Natalie Kinkel Horst Greim Clemens-Alexander Knebel Fabian Bauersachs Johann Busch Hans-Jörg Kiefl Daniel Spiel Alexander O. Marx Gernot Dietrich Christoph F. 《Der Anaesthesist》2022,71(4):307-310
Die Anaesthesiologie - 相似文献
2.
3.
4.
Dauer M Lam V Arnold H Junkmann J Kiefl R Bauer C Schnurr M Endres S Eigler A 《Journal of immunological methods》2008,337(2):97-105
Phenotypical maturation, IL-12p70 production and migration upon chemokine receptor CCR7 ligation are currently proposed as requirements for the use of human monocyte-derived dendritic cells (DC) in antitumoral vaccination. We have previously described a short-term protocol for DC generation from monocytes including stimulation with TNF-alpha, IL-1beta and PGE(2) (FastDC). These "conventional" FastDC are mature, migrate in response to CCR7 ligation and effectively stimulate autologeous T cells in vitro, but are deficient in IL-12p70 production. Here, conventional FastDC were compared to FastDC activated with different TLR ligands. High levels of IL-12p70 were induced by combined activation of FastDC with TLR4 and TLR7/8 ligands. IL-12 secretion could be maximized by additional T cell-derived stimulation. However, TLR-stimulated FastDC failed to migrate upon CCR7 ligation, independent of additional activation with CD40 ligand and IFN-gamma. The presence of PGE(2) during TLR ligation fully restored migratory capacity of FastDC, but left IL-12p70 production and activation of tumor antigen-specific cytotoxic T cells unaffected, challenging previous findings obtained with standard 7-day monocyte-derived DC. The FastDC model thus not only represents an effective tool for antitumoral vaccination, but may also provide novel insights into human DC biology. 相似文献
5.
6.
F. Mazoochian F. Schmidutz J. Kiefl A. Fottner B. Michalke R. Schierl 《Acta chirurgica Belgica》2013,113(2):123-128
Background : Hip resurfacing arthroplasty is known to increase the metal ion concentration in the serum, urine and whole blood, with potentially adverse effects on the organism. However, only few data are available about the metal ion concentrations in erythrocytes, although they are directly exposed to the higher concentrations of the serum. Methods : The ion levels of chromium, cobalt, nickel and molybdenum in erythrocytes of 25 patients with a hip resurfacing implant were analysed with high resolution ICP-sf-MS (inductively-coupled-plasma-sector-field-mass-spectrometry). The results were compared to the ion levels in the serum and urine of the patients, and also to the ion levels of 27 control persons without an implant.Results : Compared to the control group, ion levels in the erythrocytes of the hip resurfacing group were markedly increased for cobalt (0.10 vs. 3.26 μg/kg) and slightly for chromium (6.04 vs. 7.38 μg/kg). In contrast, ion levels in the serum of the hip resurfacing group were increased for cobalt (0.21 vs. 1.92 μg/l), chromium (1.48 vs. 5.64 μg/l), nickel (1.53 vs. 4.25 μg/l) and molybdenum (2.17 vs. 3.78 μg/l).Conclusion : Ion concentrations of cobalt and chromium are also increased in erythrocytes after hip resurfacing arthroplasty. Further research is required to evaluate the impact of the elevated ion levels on the erythrocytes, and to evaluate if metal ions also accumulate in other tissues of the body. 相似文献
7.
Heiser P Dempfle A Friedel S Konrad K Hinney A Kiefl H Walitza S Bettecken T Saar K Linder M Warnke A Herpertz-Dahlmann B Schäfer H Remschmidt H Hebebrand J 《Journal of neural transmission (Vienna, Austria : 1996)》2007,114(4):513-521
Summary Alterations in the serotonergic pathway have been implicated in the pathogenesis of attention-deficit/hyperactivity disorder
(ADHD). The aim of this study was to investigate seven genetic variants in three genes (serotonin transporter (5-HTT), serotonin receptor 1B (5-HTR1B) and serotonin receptor 2A (5-HTR2A)), which have previously been shown to be associated with ADHD. The polymorphisms under investigation were the 5-HTTLPR,
the VNTR in intron 2 and the 3′UTR SNP in 5-HTT, the 5-HTR1B variations 861G>C and 102T>C, and the 5-HTR2A variations His452Tyr and 1438G>A. We genotyped these variants in a sample of 102 families with 229 children with ADHD according
to DSM-IV criteria. Among the affected children, 69% fulfilled criteria for the combined type, 27% for the predominantly inattentive
type, and 4% for the predominantly hyperactive-impulsive type. Associations were tested by the pedigree transmission disequilibrium
test (PDT). All investigated polymorphisms in serotonergic candidate genes showed no association to ADHD in our sample. Earlier
studies of these polymorphisms had also shown inconsistent results, with some studies reporting significant associations and
others demonstrating no association. This discordance between studies may reflect variation in patient ascertainment criteria,
genetic heterogeneity, too low statistical power for the expected effects or false positive results in the initial reports.
We cannot rule out the possibility that other variations in the investigated genes contribute to the etiology of ADHD. 相似文献
8.
S Friedel F Fontenla Horro A K Wermter F Geller A Dempfle K Reichwald J Smidt G Br?nner K Konrad B Herpertz-Dahlmann A Warnke U Hemminger M Linder H Kiefl H P Goldschmidt W Siegfried H Remschmidt A Hinney J Hebebrand 《American journal of medical genetics. Part B, Neuropsychiatric genetics》2005,(1):96-99
Several lines of evidence indicate an involvement of brain derived neurotrophic factor (BDNF) in body weight regulation and activity: heterozygous Bdnf knockout mice (Bdnf(+/-)) are hyperphagic, obese, and hyperactive; furthermore, central infusion of BDNF leads to severe, dose-dependent appetite suppression and weight loss in rats. We searched for the role of BDNF variants in obesity, eating disorders, and attention-deficit/hyperactivity disorder (ADHD). A mutation screen (SSCP and DHPLC) of the translated region of BDNF in 183 extremely obese children and adolescents and 187 underweight students was performed. Additionally, we genotyped two common polymorphisms (rs6265: p.V66M; c.-46C > T) in 118 patients with anorexia nervosa, 80 patients with bulimia nervosa, 88 patients with ADHD, and 96 normal weight controls. Three rare variants (c.5C > T: p.T2I; c.273G > A; c.*137A > G) and the known polymorphism (p.V66M) were identified. A role of the I2 allele in the etiology of obesity cannot be excluded. We found no association between p.V66M or the additionally genotyped variant c.-46C > T and obesity, ADHD or eating disorders. This article contains supplementary material, which may be viewed at the American Journal of Medical Genetics website at http://www.interscience.wiley.com/jpages/0148-7299:1/suppmat/index.html. 相似文献
9.
Smidt J Heiser P Dempfle A Konrad K Hemminger U Kathöfer A Halbach A Strub J Grabarkiewicz J Kiefl H Linder M Knölker U Warnke A Remschmidt H Herpertz-Dahlmann B Hebebrand J 《Fortschritte der Neurologie-Psychiatrie》2003,71(7):366-377
Twin, family and adoption studies have led to a solid understanding of the contribution of both genetic and environmental factors to the development of attention deficit/hyperactivity disorder (ADHD). We review recent studies under consideration of both methodological aspects and relevant findings. Heritability estimates in the range of 0.6 - 0.8 surpass those for most other child and adolescent psychiatric disorders. First degree relatives have elevated rates for ADHD, affective disorders, conduct disorders and substance abuse and dependency. The ADHD subtype of the index patient does not predict the subtype of other family members affected with ADHD; hence non-genetic factors seemingly account for this intrafamilial variability. Because the familial rates for ADHD are not higher in families of female in comparison to male index patients, there is no indication that the genetic loading is higher in affected females. Recently, rater effects have been discussed broadly: Whereas the heritability estimates are uniformly high independent of the informant (mother, father, teacher), the correlations between quantitatively rated symptoms are low between different informants. Knowledge of the formal genetic aspects of ADHD is a prerequisite for understanding the results of recent molecular genetic studies. 相似文献
10.
John O. Ticknor Izumi Umegaki Ryan M. L. McFadden Aris Chatzichristos Derek Fujimoto Victoria L. Karner Robert F. Kiefl Shintaro Kobayashi C. D. Philip Levy Ruohong Li Gerald D. Morris Matthew R. Pearson Kazuyoshi Yoshimura Jun Sugiyama W. Andrew MacFarlane 《RSC advances》2020,10(14):8190
We have studied a mosaic of 1T-CrSe2 single crystals using β-detected nuclear magnetic resonance of 8Li from 4 to 300 K. We identify two broad resonances that show no evidence of quadrupolar splitting, indicating two magnetically distinct environments for the implanted ion. We observe stretched exponential spin lattice relaxation and a corresponding rate (1/T1) that increases monotonically above 200 K, consistent with the onset of ionic diffusion. A pronounced maximum in 1/T1 is observed at the low temperature magnetic transition near 20 K. Between these limits, 1/T1 exhibits a broad minimum with an anomalous absence of strong features in the vicinity of structural and magnetic transitions between 150 and 200 K. Together, the results suggest 8Li+ site occupation within the van der Waals gap between CrSe2 trilayers. Possible origins of the two environments are discussed.We report the first nuclear magnetic resonance measurements of an atypical transition metal dichalcogenide, CrSe2. 相似文献