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1.
Béla Nagy Zsolt Bene Zsolt Fejes Sonya L. Heltshe David Reid Nicola J. Ronan Yvonne McCarthy Daniel Smith Attila Nagy Elizabeth Joseloff György Balla János Kappelmayer Milan Macek Scott C. Bell Barry J. Plant Margarida D. Amaral István Balogh 《Journal of cystic fibrosis》2019,18(2):271-277
Background
We have recently shown that human epididymis protein 4 (HE4) levels correlate with the severity of cystic fibrosis (CF) lung disease. However, there are no data on how HE4 levels alter in patients receiving CFTR modulating therapy.Methods
In this retrospective clinical study, 3 independent CF patient cohorts (US-American: 29, Australian: 12 and Irish: 19 cases) were enrolled carrying at least one Class III CFTR CF-causing mutation (p.Gly551Asp) and being treated with CFTR potentiator ivacaftor. Plasma HE4 was measured by immunoassay before treatment (baseline) and 1–6?months after commencement of ivacaftor, and were correlated with FEV1 (% predicted), sweat chloride, C-reactive protein (CRP) and body mass index (BMI).Results
After 1?month of therapy, HE4 levels were significantly lower than at baseline and remained decreased up to 6?months. A significant inverse correlation between absolute and delta values of HE4 and FEV1 (r?=??0.5376; P?<?.001 and r?=??0.3285; P?<?.001), was retrospectively observed in pooled groups, including an independent association of HE4 with FEV1 by multiple regression analysis (β?=??0.57, P?=?.019). Substantial area under the receiver operating characteristic curve (ROC-AUC) value was determined for HE4 when 7% mean change of FEV1 (0.722 [95% CI 0.581–0.863]; P?=?.029) were used as classifier, especially in the first 2?months of treatment (0.806 [95% CI 0.665–0.947]; P?<?.001).Conclusions
This study shows that plasma HE4 levels inversely correlate with lung function improvement in CF patients receiving ivacaftor. Overall, this potential biomarker may be of value for routine clinical and laboratory follow-up of CFTR modulating therapy. 相似文献2.
László Bajnok Bertalan Kozlovszky József Varga Judit Antalffy Sándor Olvasztó Tamás Fülöp Jr. 《European journal of nuclear medicine and molecular imaging》1994,21(12):1326-1332
Technetium-99m sestamibi was used for functional investigation of the muscle perfusion of lower extremities in 35 patients
with peripheral vascular disease. The aim was to test what useful information could be obtained by additional imaging of the
legs in patients referred for risk stratification with dipyridamole myocardial scanning. Posterior images were acquired over
the thighs and calves after postocclusive reactive hyperaemia and at rest. Inter- and intraextremity ratios and differences
between the stress and rest data were used for the assessment of abnormal circulation. Arteriography was performed in every
case, and surgical procedures or transluminal angioplasty in 31 patients. To estimate diagnostic accuracy, the results of99mTc-sestamibi scintigraphy were compared with those of angiography and the functional consequences of revascularization procedures.
The sensitivity and specificity of99mTc-sestamibi scintigraphy were 55% and 25%, respectively, with an overall accuracy of 50%. Apparently methodological error
was not responsible for these poor results. Instead, a paradoxically high uptake of the radiopharmaceutical in muscles supplied
by significantly stenosed vessels was identified as the main source of both false-negative and false-positive results. This
phenomenon resembles the findings of a previous study involving delayed administration of thallium-201 after exercise. In
conclusion,99mTc-sestamibi scintigraphy has not proved sufficiently reliable to help in the management strategy for patients with peripheral
vascular disease. 相似文献
3.
István Pintér János Mátyus Zoltán Czégány Judit Harsányi Marietta Homoki Miklós Kassai Eva Kiss István Kiss Erzsébet Ladányi Lajos Locsey Lajos Major Mihály Misz Lajos Nagy Kálmán Polner Jeno Rédl István Solt Béla Tichy Marietta T?r?k Gábor Varga Gyula Wagner Imre Wórum Béla Zsoldos László Pótó Katalin Dérczy István Wittmann Judit Nagy 《Nephrology, dialysis, transplantation》2004,19(4):840-843
BACKGROUND: The diagnosis of analgesic nephropathy has improved significantly with modern imaging techniques. We reviewed a large portion of the Hungarian dialysis population to obtain additional insight into the problem. METHODS: Twenty-two participating dialysis units enrolled 1400 patients on renal replacement therapy between 1 January 1995 and 1 January 1998. Patients with no known aetiology (n = 284) were interviewed and studied with renal imaging. We assessed the presence of decreased renal mass combined with either bumpy contours, papillary calcification, or both. The subjects studied were interrogated extensively. RESULTS: Our survey suggested analgesic nephropathy in 47 of 1400 patients (3.3%), 3-fold higher than the EDTA database estimate for Hungary. The analgesics most commonly abused were phenacetin-containing mixtures. The driving symptoms were mainly headache and joint pain. Cardiovascular complications were more common than in the rest of the dialysis population, independent of smoking and lipid values (P<0.01). CONCLUSIONS: Phenacetin should be banned. Our study results support the need for longitudinal cohort and case-control studies in Hungary. 相似文献
4.
Krisztina Bencsik Judit Füvesi Zsanett Fricska-Nagy Cecília Rajda Erika Losonczi Margit T?r?k László Vécsei 《Journal of interferon & cytokine research》2006,26(2):96-100
The first pharmacon with proved efficacy for the treatment of patients with the relapsing-remitting or relapsing-progressive form of multiple sclerosis (MS) was interferon-beta1b (IFN-beta1b). In 1996, we started treating 34 relapsing-remitting (RRMS) and 2 relapsing-progressive MS (RPMS) patients with IFN-beta1b. Of these 36 patients, 28 received continuous medication for 6 years. The primary end point of the study was the effect of 6 years of continuous IFN-beta1b treatment on the annual relapse rate, the secondary end point was the change in the progression index during the 6 years, and the tertiary end point was the alteration in the expanded disability status scale (EDSS) score of the patients. Finally, we give the reasons for the dropouts. The relapse rate decreased by 80.62% (p < 0.001), the mean EDSS score increased significantly, by approximately 0.5 points, to 2.21 +/- 1.48 (p = 0.016), and the reduction in the mean progression index was 67.19% (p < 0.001). This increase of < 0.5 point in the EDSS score is appreciably different from the 3-point deterioration expected after 6 years for the natural course of the disease. The significant improvement in the progression index clearly demonstrates that 6 years of IFN-beta1b therapy slowed the progression of the disease, thereby improving the quality of life of these MS patients. 相似文献
5.
L Collet E Veuillet J Bene A Morgon 《Audiology : official organ of the International Society of Audiology》1992,31(1):1-7
The association between contralateral stimulation and evoked otoacoustic emissions (EOAEs) allows study of sound-evoked olivocochlear feedback and then of the medial olivocochlear system. A method allowing quantification of sound-evoked olivocochlear feedback is proposed. The feedback is present in almost all normal-hearing subjects, but with great interindividual variability. In sensorineural hearing loss, the feedback is present in 20 out of 21 subjects. One paradoxical clinical case is described with a unilateral increase of EOAE intensity during contralateral stimulation. 相似文献
6.
Barbarino-Monnier P.; Gobert B.; Guillet-May F.; Bene M.C.; Barbarino A.; Foliguet B.; Faure G.C. 《Human reproduction (Oxford, England)》1995,10(8):2006-2007
This case report describes a successful pregnancy and deliveryin a woman presenting with ovarian autoimmunity who had previouslybeen involved in two unsuccessful in-vitro fertilization (IVF)attempts. Ten days before a third attempt, she began a regimenof 25 mg per day of prednisolone which was continued throughoutthe whole IVF protocol. Ovulation was induced by human menopausalgonadotrophin. After administration of 5000 IU of human chorionicgonadotrophin, 18 oocytes were collected. At 48 h after inseminationwith the patient's husband's spermatozoa, four 4-cell pre-embryoswere transferred. A singleton pregnancy developed and led tothe birth of a girl who is doing well 10 months later. 相似文献
7.
Immunologic typing has demonstrated considerable heterogeneity among the acute leukemias. The most significant recent advance has been development of monoclonal antibody techniques. Some markers identified using these techniques seem to be specific for a given stage of maturation of one lymphoid or myeloid cell line. Most acute lymphoblastic leukemias (ALLs) are malignant proliferations whose differentiation appears to have become 'stuck' at one stage of maturation. Results of immunologic typing correlate well with the other clinical and biological data. For prognostic purposes, several patterns can be identified. Among B line ALLs, four varieties have been differentiated, i.e., CD10 negative ALLs, common ALLs, pre-B ALLs, and B ALLs. T ALLs include a broad spectrum of heterogeneous proliferations whose immunologic classification is made difficult by the large number of phenotypes encountered. Among acute myeloblastic leukemias (AMLs), some highly undifferentiated forms have been recognized, by means of immunologic typing, as originating in one of the myeloid cell lines. However, the nosologic and prognostic significance of these studies is less obvious than in ALLs. 相似文献
8.
Anti-HBs Cellular Immune Response in Kidney Recipients before and 4 Months after Transplantation
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Patricia Aguilar Edith Renoult Loraine Jarrosson Marie Nathalie Kolopp-Sarda Christine Prin Mathieu Gilbert C. Faure Mich
le Kessler Marie C. Bene Chantal Kohler Anne Kennel De March 《Clinical and Vaccine Immunology : CVI》2003,10(6):1117-1122
Patients with renal failure represent a population at risk for hepatitis B, since only 50 to 60% of them develop protective humoral responses after vaccination. As this could be due to an altered regulation of cellular immune responses, the objectives of the present study were to evaluate the proliferative abilities of lymphocytes from patients with chronic renal failure after stimulation in vitro with a mitogen (pokeweed mitogen [PWM]) or HBsAg. In order to differentiate between the immunodeficiency associated with renal failure and that due to immunosuppression posttransplantation, the same subjects were tested before and 4 months after kidney transplantation. The lymphoproliferation assay used was performed by flow cytometry, which is based on sequential analysis of the cell cycle and which allows analysis of cytokine production. Serologically, the group of 36 patients tested comprised 22% nonresponders, 30% poor responders, and 48% responders. Lymphocyte growth was observed for all patients after stimulation with PWM, indicating that these cells had the capacity to proliferate in vitro. The level of lymphoproliferation in response to PWM was significantly reduced after transplantation, yet both before and after transplantation, all serologic nonresponders developed cellular responses to at least two vaccines. No correlation between humoral and cellular responses was shown. Proliferating cells were lymphocytes, which mostly secreted interleukin 4 (IL-4) and IL-10 for the three serologic groups. This study suggests that even when repeated vaccination fails to induce significant antibody levels in patients with renal failure, specific HBs cellular responses develop, and these may prove to be efficient in protecting these patients against hepatitis B. 相似文献
9.
Judit Gervai Zsofia Nemoda Krisztina Lakatos Zsolt Ronai Ildiko Toth Krisztina Ney Maria Sasvari-Szekely 《American journal of medical genetics. Part B, Neuropsychiatric genetics》2005,(1):126-130
Following up the results of a previous population association study (Lakatos et al. [2000: Mol Psychiatry 5:633-637; Lakatos et al. [2002: Mol Psychiatry 7:27-31]) by analyses based on parental genetic data confirmed the link between infant attachment and the dopamine D4 receptor (DRD4) gene. Extended transmission disequilibrium tests (ETDT) were performed to determine whether biased transmission of exon III 48 basepair repeat alleles occurred to infants displaying disorganized and secure attachment behavior with their mothers. The overall allele-wise TDTs were significant for both groups (P = 0.038 and 0.020, respectively): a trend for preferential transmission of the seven-repeat allele to disorganized infants was observed (TDT(chi)(2) = 3.27, df = 1, P = 0.071), and there was a significant non-transmission of the same allele to securely attached infants (TDT(chi)(2) = 6.00, df = 1, P = 0.014). Analysis of haplotypes of the exon III repeat and the -521 C/T promoter polymorphisms in family trios showed that the transmission bias in the larger secure group was due to the low-rate transmission of the T.7 haplotype containing both the seven-repeat and the -521 T alleles (TDT(chi)(2) = 4.46, df = 1, P = 0.035). This suggests that not carrying the T.7 haplotype of the DRD4 gene may act as a resilience factor in the optimal development of early attachment. 相似文献
10.
Pongracz J Parnell S Anderson G Jaffrézou JP Jenkinson E 《Molecular immunology》2003,39(16):1013-1023
While low avidity ligation of the T cell receptor (TCR) leads to positive selection and further maturation of developing thymocytes providing the immune system with mature CD4(+) and CD8(+) (single positive) T cells, high avidity ligation triggers negative selection by apoptotic cell death and therefore the TCR repertoire is purged of autoreactive T cells. On peripheral T cells, however, high avidity ligation of the TCR triggers activation and survival not death. In the present study we used concanavalin A (Con A) and alpha-CD3 epsilon antibody to investigate a possible survival mechanism in connection with TCR ligation. Con A and alpha-CD3 epsilon were used in the study for the following reasons: (1) they both mimic the effects of high avidity TCR ligation by activating peripheral T cells, and (2) they trigger distinctively different physiological changes in developing thymocytes. While Con A supports events associated with cellular survival, alpha-CD3 epsilon induces apoptotic cell death. In our experimental system the TCR was cross-linked by Con A and alpha-CD3 epsilon in thymocytes of major histocompatibility complex (MHC) deficient thymus organ cultures, where signals from the TCR can be triggered on zero background signal level. We have found that TCR cross-linking by Con A and not by alpha-CD3 epsilon decreases the gene and protein expression of the pro-apoptotic molecule, Bad; and that Con A is capable of the activation of the survival signalling pathway including protein kinase B (Akt/PKB) independently of phosphatidyl inositol kinase (PI3K). 相似文献