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In this study, we measured the phenology of Ixodes ricinus ticks and their infection with Borrelia burgdorferi sensu lato (sl) simultaneously along an altitudinal gradient to assess the impact of climate on the phenology of ticks and on their infection with B. burgdorferi sl. From 1999 to 2001, free-living I. ricinus ticks were collected monthly by flagging vegetation at three different altitudes (620, 740, and 900 m above sea level) on the slope of a mountain in Chaumont (Neuchatel, Switzerland). I. ricinus ticks were examined for the presence of B. burgdorferi sl by using direct fluorescent antibody assay and isolation of spirochetes. Borrelia species were characterized by polymerase chain reaction followed by restriction fragment-length polymorphism. Tick density and tick phenology varied with altitude. Although the peak tick density decreased and the onset of ticks was delayed with altitude, the phenology was much more stable among years at the highest altitudes than at the lowest. The prevalence of B. burgdorferi infection in nymphs and adults decreased with altitude. The prevalence of infection differed significantly among years, and it was significantly higher in adults (30%) than in nymphs (21%). B. burgdorferi infection in adults was positively related with adult density, but this was not observed for nymphs. Five B. burgdorferi sl genospecies were successfully isolated: B. garinii, B. burgdorferi sensu stricto, B. afzelii, B. valaisiana, and B. lusitaniae. Mixed infections were obtained from five of 140 infected ticks. The greatest diversity in Borrelia species was observed at the lowest altitude where all five Borrelia species were present, whereas at the two highest altitudes, B. lusitaniae was not observed. 相似文献
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Benamor M Belhamel K Draa MT 《Journal of pharmaceutical and biomedical analysis》2000,23(6):1033-1038
A simple, rapid, and sensitive spectrophotometric method for the determination of zinc(II) is performed, based on colour reaction between the metal ion and xylenol orange in the presence of surfactant cationic cetylpyridinium chloride. The important analytical parameters and their effects on the reported system are investigated. Zinc(II) reacts with the reagent and surfactant in the ratio 1:2:4 (metal:ligand:surfactant) in the pH range 5.0–6.0 to form a ternary complex with an absorption maximum at 580 nm. The reaction was extremely rapid at room temperature, and the absorbance value remains unchanged for at least 168 h. The apparent stability constant of the complex was found to be K=1.05×1010, and the method adheres to Beer's law for 1–20 μg zinc(II) per 25 ml with apparent molar absorptivity of 1.1×104 l mol cm−1. The effect of foreign ions was tested by taking a constant concentration of metal ion and determining its concentration in the presence of large number of foreign ions. The method was applied for determination of zinc(II) in dermal ointments where excellent agreement between reported and obtained results were achieved. The relative standard deviation was better than 2%. 相似文献
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Free-living Ixodes ricinus ticks were collected from 12 different sites of canton Ticino, south of the Alps (Switzerland). Each tick was examined for the presence of Borrelia burgdorferi sensu lato (sl), the etiologic agent of Lyme borreliosis using direct fluorescent antibody assay, and isolation of the bacteria. Borreliae were characterized by PCR followed by RFLP. The abundance and infection rates of I. ricinus ticks varied greatly between the areas. Two localities were found free of Borrelia. The prevalence of infected ticks ranged from 5 to 19%. Most ticks (96%) were found infected by <50 spirochetes. Three B. burgdorferi sl species were successfully isolated: B. garinii dominated, followed by B. lusitaniae and B. valaisiana. Additionally, a mixed infection with B. garinii and B. valaisiana was observed. The distribution of the various Borrelia species in the different areas was heterogeneous. This is the first report of the presence of B. lusitaniae in I. ricinus in Switzerland. 相似文献
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H Kabiri Y el Fakir N Mahassini J Benamor S Alaziz A Elmaslout F Imani A Benosman 《Revue de pneumologie clinique》1999,55(1):21-25
We report 4 cases of malignant thoraco-pulmonary small-cell tumors (Askin tumor). Only two cases were operated. We emphasize the difficult histological diagnosis and demonstrate the importance of complete removal for survival. Prognosis remains poor. 相似文献
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Marc K Bourkadi JE Cherradi N Benamor J Hammani Z Iraqi G 《La Revue de médecine interne / fondée ... par la Société nationale francaise de médecine interne》2007,28(11):787-789
Renal involvement in sarcoidosis is rare and more often related to calcium metabolism disorders or granulomatous interstitial nephritis, glomerulonephritis is exceptional. The two cases of renal failure reported in this article illustrate the gravity of this complication, whose treatment remains difficult. 相似文献
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El Fenniri L Toossi Z Aung H El Iraki G Bourkkadi J Benamor J Laskri A Berrada N Benjouad A Mayanja-Kizza H Betts MR El Aouad R Canaday DH 《Tuberculosis (Edinburgh, Scotland)》2011,91(3):224-230
Pleural tuberculosis (TB) is a common presentation of Mycobacterium tuberculosis (MTB) infection, and despite spontaneous resolution remains a strong risk factor for reactivation pulmonary TB in a majority of individuals. This study was undertaken to further understand the characteristics of immune cells at sites of pleural TB. A significant shift toward memory CD4+ T cells with an effector phenotype and away from na?ve CD4+ T cells in pleural fluid as compared to blood mononuclear cells was found. These data suggest that effector T cells are capable of migrating to sites of active TB infection and/or the differentiation to effector phenotype T cells in situ is highly amplified. Using multi-parameter flow cytometry analysis, a significant portion of MTB-specific CD4+ T cells in the pleural space were polyfunctional demonstrating two, three or four simultaneous functions including IFN-gamma, IL-2, TNF-alpha, and or MIP-1 alpha production. A greater proportion of these polyfunctional cells were of effector memory rather than central memory phenotype. The role of these polyfunctional MTB-specific CD4+ T cells at sites of pleural TB requires further study. 相似文献
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