荧光原位杂交技术分析人结肠菌群方法研究

建立荧光原位杂交技术分析人体内结肠菌群的方法。取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。结果建立最佳实验条件为 :粪便样本收集后应尽快在 4℃下保存 ,放置时间不要超过 12小时即作处理 ;样本的适宜离心条件为 70 0g 2分钟 ;样本用多聚甲醛固定后在 - 80℃下存放时间不要超过 5个月。该方法具有较好的稳定性 ,可以有效地检出个体之间结肠菌群的差异。     

The female sensation seeker and marital sexuality.

To examine the role of sensation seeking in female sexuality, the Zuckerman's Sensation Seeking Scale was administered to a nonclinical population of married women. Comparative assessments were made between two matched samples of high and low sensation seekers on frequency of sexual intercourse, marital satisfaction, sexual desire, sexual arousal, sexual assertiveness, sexual attitudes, and sexual satisfaction. Although the high sensation seekers demonstrated significantly greater sexual desire, sexual arousability, and a more positive attitude toward sex, there were surprisingly no differences between the groups on frequency of sexual intercourse and sexual assertiveness. Also, the women classed as low sensation seekers reported greater marital and sexual satisfaction.