全文获取类型
收费全文 | 1135篇 |
免费 | 62篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 7篇 |
儿科学 | 47篇 |
妇产科学 | 7篇 |
基础医学 | 114篇 |
口腔科学 | 20篇 |
临床医学 | 70篇 |
内科学 | 564篇 |
皮肤病学 | 2篇 |
神经病学 | 27篇 |
特种医学 | 7篇 |
外科学 | 121篇 |
综合类 | 9篇 |
预防医学 | 10篇 |
眼科学 | 1篇 |
药学 | 49篇 |
中国医学 | 3篇 |
肿瘤学 | 156篇 |
出版年
2023年 | 4篇 |
2022年 | 11篇 |
2021年 | 11篇 |
2020年 | 11篇 |
2019年 | 9篇 |
2018年 | 16篇 |
2017年 | 9篇 |
2016年 | 15篇 |
2015年 | 9篇 |
2014年 | 18篇 |
2013年 | 39篇 |
2012年 | 80篇 |
2011年 | 73篇 |
2010年 | 52篇 |
2009年 | 33篇 |
2008年 | 55篇 |
2007年 | 61篇 |
2006年 | 59篇 |
2005年 | 79篇 |
2004年 | 58篇 |
2003年 | 61篇 |
2002年 | 52篇 |
2001年 | 46篇 |
2000年 | 51篇 |
1999年 | 47篇 |
1998年 | 11篇 |
1997年 | 9篇 |
1996年 | 10篇 |
1995年 | 10篇 |
1994年 | 9篇 |
1993年 | 10篇 |
1992年 | 20篇 |
1991年 | 24篇 |
1990年 | 12篇 |
1989年 | 15篇 |
1988年 | 13篇 |
1987年 | 15篇 |
1986年 | 8篇 |
1985年 | 4篇 |
1984年 | 12篇 |
1983年 | 7篇 |
1982年 | 7篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 11篇 |
1977年 | 8篇 |
1976年 | 8篇 |
1975年 | 5篇 |
1974年 | 4篇 |
排序方式: 共有1214条查询结果,搜索用时 15 毫秒
1.
2.
Radiotherapy for pediatric brain stem glioma: radiation dose, response, and survival. 总被引:2,自引:0,他引:2
T Hibi N Shitara S Genka T Fuchinoue I Hayakawa T Tsuchida K Nomura T Kondo K Takakura 《Neurosurgery》1992,31(4):643-50; discussion 650-1
An analysis of 39 patients under 20 years of age with brain stem glioma treated with radiotherapy between 1977 and 1991 was undertaken. Twenty-eight (71.2%) of the patients responded well to initial radiotherapy, and 11 (28.8%) responded poorly. Median survival for the total patient population was 10 months. Response rates and median survivals were influenced by radiation dose: 45.5% and 9 months at doses less than 4499 cGy (n = 11), 83.3% and 13 months at doses between 4500 and 5499 cGy (n = 12), 66.7% and 11.5 months at doses between 5500 and 6499 cGy (n = 9), and 100% and 10 months at doses more than 6500 cGy (n = 7). Multivariate analysis revealed the response to initial radiotherapy was the only predictor of survival with radiation doses up to 6499 cGy. Four of the patients who responded well demonstrated radiological and/or histological calcification within or around the tumor at the time of clinical deterioration. Radiation injury was confirmed in two autopsy cases. The possibility that intratumoral radiation injury causes clinical deterioration is suggested. 相似文献
3.
Akihiko Usui Fumihiko Murakami Hideki Ooshima Yasuhiro Tomita Katsuhiko Yoshida Michiaki Hibi Mitsuo Kawamura Mitsuya Muras 《Artificial organs》1997,21(7):772-778
Abstract: Cardiopulmonary support (CPS) requires durability of the oxygenator. The life span of the oxygenator is affected by various clinical factors, including patient condition, perfusion condition, and equipment usage. Predictors for the durability of oxygenators were evaluated clinically in this study. Thirty-two patients, who had undergone CPS during the last 3 years in our institute were assigned to this study. Fifty oxygenators had been used (Capiox SX in 19, CB Maxima in 23, and AL-6000 in 8). Significant predictors for the durability of oxygenators were evaluated by nonparametric survival analysis and proportional hazards regression analysis. Univariate regression analysis revealed 6 significant predictors for the life span of oxygenators. These were the oxygenator type, type of centrifugal pump, acidosis with blood pH less than 7.35, base excess less than -5, blood glutamic-oxaloacetic transaminase (GOT) levels greater than 1,000 IU, and blood lactate dehydrogenase (LDH) levels greater than 3,000 IU. After multivariate analysis, there remained only 2 significant predictors. An oxygenator used with a noncoated CPS system (Capiox SX with Capiox EBS) proved to have a significantly shorter life span than one used with a heparin-coated system (CB Maxima or AL-6000 with CB BP-80) (hazards ratio, 3.588, p = 0.0065). Patient conditions, which revealed acidosis with less than -5 of base excess, significantly shortened the life of the oxygenator (hazards ratio, 3.595, p = 0.0188). 相似文献
4.
A total of 46 patients with Turner's syndrome were treated for three years with recombinant hGH. Nineteen patients received hGH at a weekly dosage of 0.5 IU.kg-1.week-1, while 27 received 1.0 IU.kg-1.week-1 by daily sc injection. Both treatment groups showed a statistically significant growth increase during treatment. The increase in height over three years' treatment, as expressed by SD score (SDS) for chronological age, did not differ significantly between patients treated with 0.5 IU and those with 1.0 IU hGH. Seventeen of 22 patients over the age of 14 had exceeded their expected adult height. Plasma IGF-1 levels were elevated and no remarkable advances in bone age were observed during the treatment in either treatment group. There were no other significant changes in physical or laboratory examinations. No glucose intolerance was observed. These results indicate that hGH treatment is useful for accelerating growth velocity in patients with Turner's syndrome. However, further study will be required to find the best treatment dosage. 相似文献
5.
Y. Kohgo H. Hibi T. Chiba T. Shimoyama T. Muto K. Yamamura M.A. Popovsky 《Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy》2002,6(4):255-260
Abstract: Recently, successful results of ulcerative colitis (UC) treatments with leukocyte apheresis have been reported by several institutes. To certify the efficacy of leukocyte apheresis in refractory UC patients, a multicenter open label trial was conducted, and results were analyzed. Fifty patients diagnosed with active steroid‐resistant UC were enrolled in this study from 14 medical centers. Using a centrifugal cell separator (Component Collection System, Haemonetics), leukocyte apheresis was performed once a week for 5 weeks. General conditions and abdominal symptoms were recorded daily, and laboratory tests were followed weekly. Changes of colonoscopic and histological manifestations of luminal activity through the study period were evaluated. At the end of the study period, stool frequency was decreased to less than 4 times a day in 68.4% (26 of 38) and serum C‐reactive protein (CRP) concentration was normalized in 56.7% (17 of 30) of the patients. Colonoscopic remission was achieved in 57.7% (26 of 45), and histological improvement was noted in 54.1% (20 of 37) of the patients tested. Improved disease activity was demonstrated in 74% (37 of 50) of the patients by general assessment criteria. Analysis of the trial data confirmed the valid clinical efficacy of leukocyte apheresis by centrifugal cell separator in refractory UC patients. 相似文献
6.
7.
8.
Alteration of T cell maturation and proliferation in the mouse thymus induced by serum factors from patients with ulcerative colitis. 下载免费PDF全文
M Watanabe S Aiso T Hibi N Watanabe Y Iwao T Yoshida H Asakura S Tsuru M Tsuchiya 《Clinical and experimental immunology》1987,68(3):596-604
Recently it has been reported that patients with ulcerative colitis (UC) often have thymus abnormalities, although the precise mechanisms which induce those abnormalities remain unclear. We have examined the effect of serum fractions from patients with UC and other colonic diseases on mouse thymus to clarify the possible existence of factors which have thymus growth activity. These fractions were separated from sera of patients with UC by gel filtration and anion exchange high performance liquid chromatography. In mice given UC serum fractions; (i) remarkable increases in weight and total cell number of the thymus were observed from day 4 to day 9; (ii) a significant increase in the number of peanut agglutinin (PNA)+ thymus cells was demonstrated using flow cytometry on day 9; (iii) on quantitative analysis of surface antigens the percentage of Lyt-2+ thymus cells decreased and that of L3T4+ thymus cells increased remarkably on day 13; the number of bright Thy-1.2+ cells and of dull Lyt-1+ cells increased. In contrast, the serum fractions from patients with other colonic diseases and from normal persons caused little change in mouse thymus throughout the study. The results suggest that factors fractionated from the serum of patients with UC disturb intra-thymic T cell maturation and enhance the proliferation of thymus cells. 相似文献
9.
Mesophyll protoplasts were isolated from the primary leaves of cowpeas by a one-step procedure using a mixture of Macerozyme and Cellulase. The protoplasts were inoculated with cowpea mosaic virus and virus multiplication was shown to occur by measuring the virus infectivity at various times after inoculation. Eighty to ninety-six percent of the protoplasts were infected as shown by fluorescent antibody staining. More than 108 progeny virus particles were produced per infected protoplast. A cytopathic structure similar to the structure found in CPMV infected leaf cells occurred in infected protoplasts. Poly-l-ornithine was not essential for CPMV infection of the cowpea mesophyll protoplasts, but it had a stimulating effect. This is the first protoplast system after the tobacco mesophyll protoplast system suitable for virus infection studies. As cowpeas belong to a plant family other than tobacco, the new protoplasts allow comparative infection studies with plant viruses. 相似文献
10.
The Serum Factor from Patients with Ulcerative Colitis that Induces T Cell Proliferation in the Mouse Thymus Is Interleukin-7 总被引:3,自引:0,他引:3
Mamoru Watanabe Noriaki Watanabe Yasushi Iwao Haruhiko Ogata Takanori Kanai Yoshitaka Ueno Masaharu Tsuchiya Hiromasa Ishii Sadakazu Aiso Sonoko Habu Toshifumi Hibi 《Journal of clinical immunology》1997,17(4):282-292
The disturbance of immune regulatory T cells is related to the pathogenesis of ulcerative colitis. Here we demonstrated and characterized the serum factor from ulcerative colitis patients that induced proliferation of intrathymic T cells. The factor isolated from the patient sera by a combination of gel filtration and anion-exchange chromatography induced proliferation of CD4+CD8– intrathymic T cells in the organ-cultured embryonic mouse thymus. Purification and amino acid sequence analysis of the serum factor demonstrated that the N-terminal 12 sequence was homologous to that of interleukin-7. SDS-PAGE and Western blot confirmed that purified serum factor was interleukin-7. Enzyme immunoassay demonstrated that the serum interleukin-7 concentration was significantly increased in the patients. PCR and Southern blot hybridization demonstrated that interleukin-7 mRNA expression was increased in the thymus tissues from patients but decreased in the colonic mucosa. Since interleukin-7 is a crucial cytokine for proliferation and differentiation of T cells in the thymus, the present study indicates that interleukin-7 may contribute to the disturbance of immune regulatory T cells in ulcerative colitis. 相似文献