全文获取类型
收费全文 | 852篇 |
免费 | 47篇 |
国内免费 | 46篇 |
专业分类
耳鼻咽喉 | 12篇 |
儿科学 | 20篇 |
妇产科学 | 10篇 |
基础医学 | 128篇 |
口腔科学 | 4篇 |
临床医学 | 109篇 |
内科学 | 160篇 |
皮肤病学 | 5篇 |
神经病学 | 73篇 |
特种医学 | 145篇 |
外国民族医学 | 1篇 |
外科学 | 80篇 |
综合类 | 26篇 |
一般理论 | 2篇 |
预防医学 | 37篇 |
眼科学 | 15篇 |
药学 | 66篇 |
肿瘤学 | 52篇 |
出版年
2022年 | 8篇 |
2021年 | 9篇 |
2020年 | 9篇 |
2019年 | 15篇 |
2018年 | 14篇 |
2017年 | 12篇 |
2016年 | 14篇 |
2015年 | 12篇 |
2014年 | 14篇 |
2013年 | 36篇 |
2012年 | 30篇 |
2011年 | 27篇 |
2010年 | 24篇 |
2009年 | 35篇 |
2008年 | 49篇 |
2007年 | 62篇 |
2006年 | 43篇 |
2005年 | 48篇 |
2004年 | 35篇 |
2003年 | 32篇 |
2002年 | 37篇 |
2001年 | 34篇 |
2000年 | 23篇 |
1999年 | 18篇 |
1998年 | 26篇 |
1997年 | 22篇 |
1996年 | 9篇 |
1995年 | 16篇 |
1994年 | 12篇 |
1993年 | 12篇 |
1992年 | 18篇 |
1991年 | 19篇 |
1990年 | 13篇 |
1989年 | 9篇 |
1988年 | 10篇 |
1987年 | 10篇 |
1986年 | 7篇 |
1985年 | 10篇 |
1984年 | 11篇 |
1983年 | 9篇 |
1982年 | 9篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1979年 | 11篇 |
1977年 | 6篇 |
1976年 | 9篇 |
1975年 | 7篇 |
1974年 | 6篇 |
1972年 | 6篇 |
1970年 | 5篇 |
排序方式: 共有945条查询结果,搜索用时 15 毫秒
1.
2.
3.
Persistent asthma after accidental exposure to ethylene oxide. 总被引:2,自引:0,他引:2
4.
P. Lambin A. Gervais M. Levy E. Defendini M. Dubarry P. Lebon P. Rouger E. Schuller 《Journal of neuroimmunology》1991,35(1-3):179-189
Serum and cerebrospinal fluid (CSF) of 50 neurological patients (24 multiple sclerosis (MS), ten acquired immunodeficiency syndrome (AIDS) and 16 other neurological diseases (OND)) and ten controls were analyzed by enzyme-linked immunosorbent assay (ELISA) for IgG subclass quantification and for the calculation of intrathecal synthesis (ITS). Total IgG was determined by two methods: electroimmunodiffusion (EID) and ELISA. A highly significant correlation was established between both methods. The existence of ITS was proved by the IgG/albumin ratio, the IgG index, Tourtellotte's formula, and Schuller's formula. In AIDS patients all IgG subclasses showed an increase in the CSF, whereas in sera only the IgG1 was significantly increased. CSF of MS patients showed a predominant increase of IgG1 whereas no significant modification of IgG subclasses was observed in sera. In most of the AIDS patients there was an ITS of IgG1, IgG3 and IgG4, but rarely (3/10) IgG2. In contrast, a polyclonal ITS of IgG was exceptional (1/24) in MS patients. No significant correlation could be established between clinical data and IgG subclass ITS in MS. The variations of each IgG subclass in serum and in ITS were not significantly correlated. Measurement of each IgG subclass and calculation of ITS seems essential in order to analyze any subclass antibody repertory inside the central nervous system. 相似文献
5.
F.?W. Ahnefeld J. Barth W. Dick A. Doenicke T. Fuchs H. Gervais H. Laubenthal H. L?llgen W. Lorenz H.?H. Mehrkens G.?H. Meuret H. M?llmann S. Piepenbrock B. Przybilla R. Ring W. Schmutzler G. Schultze-Werninghaus J. Schüttler H.?P. Schuster P. Sefrin M. Tryba J. Zander M. Zenz 《Der Anaesthesist》1994,43(4):211-222
6.
7.
8.
Jocelyne Daigneault Gervais Aubin Fernand Simard Marc De Braekeleer 《Clinical genetics》1991,40(4):298-303
Cystic fibrosis (CF) is an autosomal recessive disorder with a prevalence at birth estimated at 1/2000-1/2500 livebirths in Caucasian populations. Some 127 CF individuals are known in Saguenay-Lac-St-Jean (SLSJ), a geographically isolated region of Quebec. The prevalence at birth was estimated at 1/902 live borns, and the carrier rate was estimated at 1/15 inhabitants in the SLSJ region. The mean inbreeding coefficient was only slightly elevated in the CF group compared with three control groups, and was due to remote consanguinity. The mean kinship coefficient was 2.4 times higher in the CF group than in the control groups. In SLSJ region, the places of origin of the CF individuals and their parents did not show a clustered nonuniform distribution. Endogamy was not higher in the CF group than in control groups. 相似文献
9.
Calcium ionophore-induced acrosome reaction correlates with fertilization rates in vitro in patients with teratozoospermic semen 总被引:5,自引:3,他引:5
The aim of this study was to determine the relationship between calcium
ionophore A23187-induced acrosome reaction (AR) and sperm fertilizing
ability. Semen samples remaining after preparation for standard IVF were
studied in 109 patients who had sperm concentrations > or =20 x
10(6)/ml. Ionophore-induced AR was performed on motile spermatozoa selected
by centrifugation on a Percoll gradient. Semen analysis was performed using
standard methods. Patients with higher (>50%, n = 76) fertilization
rates had significantly higher ionophore-induced AR than patients with
lower (<50%, n = 33) fertilization rates (49 +/- 14 versus 38 +/- 21%, P
< 0.05). When the data from all patients were analysed by logistic
regression, only the percentage sperm motility in insemination medium and
ionophore-induced AR were significantly related to fertilization rates.
Similar results were also obtained when the data from a subgroup of
patients with poor (<15% normal) sperm morphology were analysed.
However, when patients with normal sperm morphology > or =15% were
analysed separately, only sperm count and the percentage of spermatozoa
with progressive motility in semen were significantly related to
fertilization rates. In conclusion, ionophore- induced AR was significantly
related to fertilization rates in vitro mainly in patients with
teratozoospermic semen. Tests for ionophore- induced AR may provide
additional information about sperm fertilizing ability but may not indicate
specific defects of the physiological AR.
相似文献
10.
Cloning of the genome of a densovirus and rescue of infectious virions from recombinant plasmid in the insect host Spodoptera littoralis 总被引:1,自引:0,他引:1
We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparable percentage of larvae became infected following inoculation of equivalent amounts of purified virion DNA or cloned viral DNA. Virions extracted from transfected larvae were indistinguishable from wild-type (wt) virions with regard to their biophysical and biological properties. In particular, rescued virions were as infectious as wt virions and showed identical restriction profiles of their genome. In contrast, subcloning of JcDNV DNA deleted at both extremities of a sequence of ca 250 or ca 100 bp resulted in the inability of the recombinant plasmids to initiate a viral infection. These data suggest that, as for vertebrate parvoviruses, the inverted terminal repeats display essential functions in the rescue process and replicative cycle of densoviruses. This is the first report of the molecular cloning of the infectious genome from an insect parvovirus, and more generally from an invertebrate virus. pBRJ should provide an efficient tool to further define the organization of the JcDNV genome and compare it to other parvoviruses. 相似文献