全文获取类型
收费全文 | 734篇 |
免费 | 60篇 |
国内免费 | 3篇 |
专业分类
儿科学 | 2篇 |
妇产科学 | 11篇 |
基础医学 | 121篇 |
临床医学 | 64篇 |
内科学 | 211篇 |
皮肤病学 | 7篇 |
神经病学 | 49篇 |
特种医学 | 23篇 |
外科学 | 98篇 |
综合类 | 1篇 |
一般理论 | 2篇 |
预防医学 | 37篇 |
眼科学 | 2篇 |
药学 | 66篇 |
中国医学 | 1篇 |
肿瘤学 | 102篇 |
出版年
2023年 | 1篇 |
2022年 | 11篇 |
2021年 | 14篇 |
2020年 | 6篇 |
2019年 | 14篇 |
2018年 | 8篇 |
2017年 | 13篇 |
2016年 | 13篇 |
2015年 | 32篇 |
2014年 | 36篇 |
2013年 | 37篇 |
2012年 | 67篇 |
2011年 | 68篇 |
2010年 | 31篇 |
2009年 | 33篇 |
2008年 | 61篇 |
2007年 | 48篇 |
2006年 | 59篇 |
2005年 | 65篇 |
2004年 | 53篇 |
2003年 | 46篇 |
2002年 | 41篇 |
2001年 | 5篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1994年 | 7篇 |
1993年 | 1篇 |
排序方式: 共有797条查询结果,搜索用时 0 毫秒
1.
Reliable procedure for the production of 1-[(11)C]acetate in GMP conditions was developed based on a combination of the captive-solvent Grignard reaction conducted in the sterile catheter followed by the convenient solid-phase extraction purification on a series of ion-exchange cartridges. The described procedure proved to be reliable in more than 30 patient productions. The process provides stable radiochemical yields (65% EOB) of sodium acetate (1-[(11)C]) of the Ph.Eur. quality (radiochemical purity better than 95%) in a short time (5 min). 相似文献
2.
Dmitri Artemov Zaver M. Bhujwalla Ross J. Maxwell John R. Griffiths Ian R. Judson Martin O. Leach Jerry D. Glickson 《Magnetic resonance in medicine》1995,34(3):338-342
The anticancer agent temozolomide labeled with 13C (8-Carbamoyl-3-13C-methylimidazo-[5,1-d]-1,2,3,5-tetrazin-4-(3H)-one), was noninvasively detected in subcutaneous RIF-1 tumors by a selective cross polarization 13C NMR method, at a field strength of 9.4T. Pharmacokinetics of the drug, at a dose of 150 mg/kg, were determined for intravenous and intraperitoneal modes of administration (three animals per mode). The half-life of the drug in the tumors was approximately 60 min. The uptake and clearance of the drug, however, varied significantly between individual hosts, for both modes of administration. These results demonstrate the feasibility of obtaining pharmacokinetics of anticancer agents for individual tumors without the need for a label that might modify drug activity (e.g., fluorine). The variability of the in vivo measurements, even within the same tumor model, demonstrates the necessity of directly monitoring the tumor to evaluate drug pharmacokinetics. 相似文献
3.
Dmitri A Rusakov 《The Neuroscientist》2006,12(4):317-326
Classically, a high-power association relates the neurotransmitter release probability to the concentration of presynaptic Ca2+. Activated by the action potential waveform, voltage-gated Ca2+ channels mediate Ca2+entry into presynaptic terminals. Inside the terminal, Ca2+ ions rapidly bind to endogenous intracellular buffers and could trigger Ca2+ release from internal Ca2+ stores. The resulting space-time profile of free Ca2+ determines the time course and probability of neurotransmitter release through the interaction with molecular release triggers strategically located in the vicinity of release sites. Following a rapid concentration transient, excess Ca2+ has to be removed from the cytosol through the process involving Ca2+ uptake by the endoplasmatic reticulum stores, sequestration by mitochondria, and/or extrusion into the extracellular medium. The ongoing synaptic activity could affect any of the multiple factors that shape presynaptic Ca2+ dynamics, thus arbitrating use-dependent modification of the neurotransmitter release probability. Here we present an overview of major players involved in Ca2+-dependent presynaptic regulation of neurotransmitter release and discuss the relationships arising between their actions. 相似文献
4.
Paul De Sutter Dmitri Dozortsev Philippe Vrijens Rita Desmet Marc Dhont 《Journal of assisted reproduction and genetics》1994,11(8):382-388
Purpose
Treatment of aged human oocytes by puromycin allows a high rate of parthenogenetic activation and development until the first cleavage division. This technique was used for the study of the chromosome complement of oocytes which remained unfertilized after in vitro fertilization. Three hundred four unfertilized oocytes were treated with 10 Μg/ml puromycin for 6–8 hr and further cultured for 12–15 hr.Results
Activation occurred in 90.5% of the oocytes. Heterozygous diploids with two pronuclei predominated (61%), which is in contrast to the mouse, where the majority of oocytes activated by puromycin are uniform haploids (89%).Conclusions
Therefore we conclude that puromycin treatment induces retention of the second polar body in human oocytes, unlike in mouse oocytes treated in the same way. Chromosome analysis performed on 182 oocytes suggested a nondisjunction (ND) rate for the second meiotic division of 12.7%. This is a low figure considering the fact that puromycin itself has been reported to induce nondisjunction. For the first meiotic division a ND rate of only 5.6% was found. This rate is lower than the one found in metaphase II arrested oocytes and we believe that this difference is due to the technical differences between the study of meiotic and that of mitotic chromosomes. 相似文献5.
Zaver M Bhujwalla Dmitri Artemov Kshama Natarajan Meiyappan Solaiyappan Peggy Kollars Paul E G Kristjansen 《Clinical cancer research》2003,9(1):355-362
PURPOSE: The availability of noninvasive techniques to detect the effects of antiangiogenic agents is critically important for optimizing treatment of cancer with these agents. Magnetic resonance imaging (MRI) is one such noninvasive technique that is routinely used clinically. EXPERIMENTAL DESIGN: In this study, we have evaluated the use of MRI of the intravascular contrast agent albumin-GdDTPA to detect the effects of the antiangiogenic agent TNP-470 on the vascular volume and permeability of the MatLyLu prostate cancer model. RESULTS: TNP-470-treated tumors demonstrated a significant decrease of vascular volume, as well as a significant reduction in vascular and permeable regions, compared with volume-matched control tumors. Although the fractional volume of permeable regions in the tumor decreased, the average value of tumor permeability did not decrease significantly. This was attributable to increase in permeability in some regions of the tumor. These regions were mostly associated with low vascular volume. ELISA assays of control and treated MatLyLu tumors also detected a significant increase of vascular endothelial growth factor in the TNP-470-treated tumors. CONCLUSION: MRI detected significant changes in tumor vascular characteristics after treatment with TNP-470. 相似文献
6.
7.
Liu J Yang C Simpson C Deryckere D Van Deusen A Miley MJ Kireev D Norris-Drouin J Sather S Hunter D Korboukh VK Patel HS Janzen WP Machius M Johnson GL Earp HS Graham DK Frye SV Wang X 《ACS medicinal chemistry letters》2012,3(2):129-134
Ectopic Mer expression promotes pro-survival signaling and contributes to leukemogenesis and chemoresistance in childhood acute lymphoblastic leukemia (ALL). Consequently, Mer kinase inhibitors may promote leukemic cell death and further act as chemosensitizers increasing efficacy and reducing toxicities of current ALL regimens. We have applied a structure-based design approach to discover novel small molecule Mer kinase inhibitors. Several pyrazolopyrimidine derivatives effectively inhibit Mer kinase activity at sub-nanomolar concentrations. Furthermore, the lead compound shows a promising selectivity profile against a panel of 72 kinases and has excellent pharmacokinetic properties. We also describe the crystal structure of the complex between the lead compound and Mer, opening new opportunities for further optimization and new template design. 相似文献
8.
9.
10.
Ekaterina Lukianova-Hleb Sarah Bezek Reka Szigeti Alexander Khodarev Thomas Kelley Andrew Hurrell Michail Berba Nirbhay Kumar Umberto D’Alessandro Dmitri Lapotko 《Emerging infectious diseases》2015,21(7):1122-1127
A fast, precise, noninvasive, high-throughput, and simple approach for detecting malaria in humans and mosquitoes is not possible with current techniques that depend on blood sampling, reagents, facilities, tedious procedures, and trained personnel. We designed a device for rapid (20-second) noninvasive diagnosis of Plasmodium falciparum infection in a malaria patient without drawing blood or using any reagent. This method uses transdermal optical excitation and acoustic detection of vapor nanobubbles around intraparasite hemozoin. The same device also identified individual malaria parasite–infected Anopheles mosquitoes in a few seconds and can be realized as a low-cost universal tool for clinical and field diagnoses. 相似文献