经皮椎体成形术治疗新鲜骨质疏松性椎体压缩骨折

目的探讨经皮椎体成形术(percutaneous vertebroplasty,PVP)治疗新鲜骨质疏松性椎体压缩骨折的疗效。方法 2011年1月~2012年12月共治疗骨质疏松性单椎体压缩骨折38例,其中男7例,女31例;年龄55~80岁(平均65.1岁)。患者取俯卧位局麻下行单侧穿刺PVP。测量椎体高度的恢复情况和后凸角改善情况,视觉模拟量表(visual analogue scale,VAS)评分评价疼痛缓解程度,活动能力评分检测活动改善情况,并对并发症进行分析。结果所有手术均顺利完成,每个椎体平均填充骨水泥3.5 m L。随访时间均12个月。椎体前壁高度由术前(19.2±5.7)mm改善至术后(20.0±5.7)mm,椎体中间高度由术前(19.2±5.1)mm改善至术后(20.2±5.0)mm,差异有统计学意义(P0.05);椎体后壁高度和Cobb角术前与术后相比,差异无统计学意义(P0.05);VAS评分术前8.3±1.1,术后12个月时为1.1±0.6,术前、术后差异有统计学意义(P0.05)。活动能力评分术前3.1±0.9,术后12个月时为1.1±0.3,术前、术后差异有统计学意义(P0.05)。骨水泥渗漏9例。结论 PVP可有效治疗新鲜骨质疏松性椎体压缩骨折,创伤小,减轻疼痛,改善功能,无重大并发症。     

利用六通道光纤传感系统监测盐酸胺碘酮片的溶出度

目的:利用六通道光纤药物溶出度过程监测仪,建立盐酸胺碘酮片剂的溶出度测定方法。方法:将6个光纤公共端部分别降入盛有900 mL0.25%十二烷基硫酸钠溶液的溶出杯,分别投入盐酸胺碘酮片,采用桨法,转速100 r·min~(-1),测定波长305 nm,参比波长为550 nm,通过计算机监视溶出过程。结果:光程6 mm,在305 nm 处胺碘酮于20.00～200.00 mg·L~(-1)范围内线性关系良好(r=0.9999),回收率试验与中国药典2000年版溶出度测定方法比较,2种测定方法无统计学意义(P>0.05)。结论:该法实现同时检测6片胺碘酮的溶出度,可直接从溶出曲线上提取相关参数;六通道光纤药物溶出过程监测系统原位、实时、连续、定量地反映了溶出迅速的特性,获得的数据信息更加完整、真实,相应软件的使用实现了体外溶出过程监测的自动化、智能化。     

一阶导数光谱法测定奈韦拉平片含量

目的:建立测定奈韦拉平片中奈韦拉平含量的方法。方法:采用一阶导数UV光谱法,以275 nm(峰)与299 nm(谷)振幅值为定量依据,进行定量分析。该法与RP-HPLC法和零阶导数光谱法进行了对比。色谱柱为Shimadzu ODS(250 mm×4.6 mm,5μm),流动相为甲醇-水(用磷酸调pH=3),(60∶40),流速为1.0 mL.min-1;紫外检测波长282 nm,柱温35℃,采用外标法计算含量。结果:结果表明奈韦拉平浓度在2.5～25.0μg.mL-1范围内与振幅值呈良好的线性关系(r=0.9997),平均回收率为99.5%(RSD=1.5%)。统计检验发现HPLC法与一阶导数UV法无显著性差异,而与零阶导数法有显著性差异。结论:一阶导数UV光谱法可靠,简便快速,可有效消除赋形剂干扰,结果准确,可用于奈韦拉平片的质量控制。     

Synthesis of fluorescent drug molecules for competitive binding assay based on molecularly imprinted polymers

Fluorescent immunosorbent assay (FIA) is very promising for sensitive and selective analysis in bio-medical applications. Here, we proposed an assay, using fluorescent engineering of analytes and the corresponding molecularly imprinted polymers (MIPs) as a plastic antibody. Three drug molecules (metronidazole, zidovudine and lamivudine) were condensed with 9-aminoacridine, using succinic anhydride as a spacer. The target products were characterized with ¹H-NMR, IR and mass spectrometry. UV-vis absorption and fluorescent properties of the fluorophore-labeled drug molecules were investigated. Feasibility of the fluorescent biomimetic immunosorbent assay based on MIPs was demonstrated in the solution. This work will provide sound foundation for the future application in real sample.

Drug molecules (metronidazole, zidovudine and lamivudine) were successfully labelled with a fluorescent reagent and used to develop fluorescent biomimetic immunosorbent assays using molecularly imprinted polymers in the place of natural antibody.     

Effects of soybean peptide on immune function, brain function, and neurochemistry in healthy volunteers

ObjectiveSoybeans, an excellent source of dietary peptides, have beneficial effects on health. We investigated the effect of the soybean peptide on immune function, brain function, and neurochemistry in healthy volunteers.MethodsNear-infrared spectroscopy (NIRS) was used to analyze brain cerebral blood flow. The A and DA levels in the serum were analyzed by ELISA kit. The total number of leukocytes was recorded with a standard counter. Flow cytometry was used to assess lymphocyte subset levels.ResultsCell numbers were upregulated in the group that had fewer leukocytes but downregulated in the group with more leukocytes. For the lymphocyte-rich type, lymphocyte counts tended to decrease, accompanied by an increase in granulocyte numbers. For the granulocyte-rich type, granulocyte counts tended to increase, but lymphocyte counts also increased. The numbers of CD11b⁺ cells and CD56⁺ cells increased significantly. Soybean peptide decreased the adrenalin level in plasma but increased the level of dopamine. Near-infrared spectroscopy showed significant increases in the amplitudes of θ, α-2, and β-L frequency bands after the ingestion of peptides.ConclusionSoybean peptides can modulate cellular immune systems, regulate neurotransmitters, and boost brain function.     

Feedback-based error monitoring processes during musical performance: an ERP study

Auditory feedback is important in detecting and correcting errors during sound production when a current performance is compared to an intended performance. In the context of vocal production, a forward model, in which a prediction of action consequence (corollary discharge) is created, has been proposed to explain the dampened activity of the auditory cortex while producing self-generated vocal sounds. However, it is unclear how auditory feedback is processed and what neural mechanism underlies the process during other sound production behavior, such as musical performances. We investigated the neural correlates of human auditory feedback-based error detection using event-related potentials (ERPs) recorded during musical performances. Keyboard players of two different skill levels played simple melodies using a musical score. During the performance, the auditory feedback was occasionally altered. Subjects with early and extensive piano training produced a negative ERP component N210, which was absent in non-trained players. When subjects listened to music that deviated from a corresponding score without playing the piece, N210 did not emerge but the imaginary mismatch negativity (iMMN) did. Therefore, N210 may reflect a process of mismatch between the intended auditory image evoked by motor activity, and actual auditory feedback.     

罗布麻叶中多酚的提取工艺和抗氧化活性的研究

目的确定罗布麻叶中多酚的最佳提取工艺,并分析其抗氧化活性。方法以乙醇为溶剂利用正交实验法优化提取多酚的条件,并通过清除羟自由基、DPPH.自由基和超氧阴离子自由基以及还原力实验来评价罗布麻叶中多酚体外抗氧化能力,并与VC进行了比较。结果乙醇浓度为50%、料液比为1∶20、提取时间为2 h、提取温度为50℃时罗布麻叶多酚的提取率最高,其含量达4.09%。罗布麻叶多酚具有一定的还原力,其还原力比VC的稍强。罗布麻叶多酚对.OH,DPPH.和O2-.都有一定清除能力,其IC50值分别为0.08,0.004和0.048 mg/ml,而VC的分别为0.13,0.027和0.023 mg/ml。结论罗布麻叶中多酚的最佳提取工艺为A1B1C2D1,在实验条件下,罗布麻叶多酚具有一定的抗氧化作用。     

阿米乐努西达日蜜膏中没食子酸的含量测定

摘要目的建立测定维吾尔医药阿米乐努西达日蜜膏没食子酸含量的方法。方法采用高效液相色谱法,色谱柱：phenomenex C18（4.6 mm×250 mm,5 μm）;检测波长：273 nm;流动相：甲醇 0.4%磷酸水溶液（10：90）;流速：1.0 mL•min－1;进样量：10 μL;柱温：25 ℃。结果没食子酸在0.106～0.530 μg范围内峰面积与进样量具有良好的线性关系（r＝0.999 7）,平均加样回收率为97.9%（n＝9）,RSD＝1.13%。结论该方法操作简便、准确、灵敏度高、重复性好,可为阿米乐努西达日蜜膏质量评价提供参考。     

Cytotoxicity of abnormal Savda Munziq aqueous extract in human hepatoma (HepG2) cells

Abnormal Savda Munziq (ASMq) is a traditional Uighur medicinal herbal preparation commonly used to treat diseases such as diabetes, cardiovascular diseases, chronic asthma and especially digestive cancer. Earlier studies have shown that ASMq is a free radical scavenger and could prevent mitochondrial and DNA oxidative damage. In this study, we tested the effects of aqueous extract of ASMq on human hepatoma cells (HepG2) to explore the possible mechanism of its putative anticancer properties. Aqueous extract of ASMq was tested on HepG2 proliferation (MTT assay) at 72 h, cell viability at 48 h (neutral red assay), lactate dehydrogenase release over 48 or 72 h as a measure of cytoplasmic leakage, lipid peroxidation (malondialdehyde-thiobarbituric acid adducts) at 48 h, and incorporation of [3H]-leucine, [3H]-thymidine and [3H]-uridine into cellular protein, DNA and RNA, respectively, at 24 or 48 h to assess the inhibition effects to cellular macromolecule synthesis. Our results showed a significant (P < 0.05) time- and concentration-dependent inhibition of HepG2 proliferation and viability, with increased cytoplasmic leakage, and time- and concentration-dependent inhibition of protein, DNA and RNA synthesis. No lipid peroxidation was found at these concentrations. The results of the present study suggest that the putative anticancer mechanisms of ASMq may at least involve cytotoxicity.     

新疆假龙胆中有效成分提取工艺的研究

目的：优选龙胆的最佳提取工艺。方法：比较冷浸提取、超声提取、加热回流提取、渗漉提取和煎煮提取等5种提取方式。采用L9（3^4）正交试验,以龙胆苦苷、龙胆总苷的提出率和干膏得率为考察指标,通过多指标综合评分法进行数据分析,对影响龙胆提取工艺的因素进行了研究。结果：龙胆的最佳提取工艺为：龙胆药材用70％乙醇徊热回流提取3次,每次1h．溶剂用量分别为10倍、8倍、8倍。结论：优选得到的提取工艺稳定可行,适合工业化生产。