全文获取类型
收费全文 | 461篇 |
免费 | 44篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 7篇 |
妇产科学 | 10篇 |
基础医学 | 75篇 |
口腔科学 | 7篇 |
临床医学 | 53篇 |
内科学 | 113篇 |
皮肤病学 | 10篇 |
神经病学 | 58篇 |
特种医学 | 9篇 |
外科学 | 49篇 |
综合类 | 5篇 |
一般理论 | 1篇 |
预防医学 | 37篇 |
眼科学 | 6篇 |
药学 | 24篇 |
肿瘤学 | 36篇 |
出版年
2023年 | 3篇 |
2022年 | 14篇 |
2021年 | 35篇 |
2020年 | 16篇 |
2019年 | 23篇 |
2018年 | 25篇 |
2017年 | 15篇 |
2016年 | 16篇 |
2015年 | 25篇 |
2014年 | 26篇 |
2013年 | 31篇 |
2012年 | 39篇 |
2011年 | 35篇 |
2010年 | 25篇 |
2009年 | 19篇 |
2008年 | 30篇 |
2007年 | 25篇 |
2006年 | 19篇 |
2005年 | 22篇 |
2004年 | 11篇 |
2003年 | 15篇 |
2002年 | 17篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1983年 | 1篇 |
1968年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1955年 | 1篇 |
1945年 | 1篇 |
1942年 | 1篇 |
1940年 | 1篇 |
排序方式: 共有505条查询结果,搜索用时 15 毫秒
1.
2.
3.
Pallier C Tebourbi L Chopineau-Proust S Schoevaert D Nordmann P Testart J Courtot AM 《Human reproduction (Oxford, England)》2002,17(5):1281-1287
BACKGROUND: The effect of viral particles on the motility of human sperm and the relationship between sperm and virus are of importance particularly in assisted fertilization. METHODS: We incubated ejaculated sperm with or without seminal fluid with either herpes simplex virus type 2 (HSV2) or human cytomegalovirus (HCMV). For each experiment, 5 x 10(5) sperm were incubated with a viral load of between 10(4) and 10(6) plaque-forming units. RESULTS: We detected no apparent variations in the percentage of motile forms when sperm were incubated with either HSV2 or HCMV. Using a computer-aided semen analysis system, a slight difference was reported in the percentage of motile forms when seminal fluid-free sperm were incubated with HSV2 (57.18 versus 64.43 in the control). Although the mean amplitude of lateral head displacement and the curvilinear velocity were significantly higher in infected sperm, the difference in straight line velocity was not statistically significantly different. Few viral particles (HSV2 or HCMV) adhered to the sperm membrane in the presence of seminal fluid. However, more particles stuck when in the absence of seminal fluid, particularly with HSV2 (8% of sperm sections for HSV2; 4% for HCMV). CONCLUSIONS: The relationship between sperm and viruses depends on the type of virus present as well as the presence or absence of seminal fluid. Motility is not a good enough criterion on which to prove the presence of viral elements, either in the medium or on the sperm. 相似文献
4.
Martin C Al-Qaoud KM Ungeheuer MN Paehle K Vuong PN Bain O Fleischer B Hoerauf A 《Medical microbiology and immunology》2000,189(2):67-74
The pathways conferring immunity to human filariases are not well known, in part because human-pathogenic filariae do not
complete a full life cycle in laboratory mice. We have used the only fully permissive infection of mice with filariae, i.e.,
infection of BALB/c mice with the rodent filarial nematode Litomosoides sigmodontis. Our previous results showed that worm development is inversely correlated with Th2 cytokine production and eosinophilia.
The scope of the present study was to directly elucidate the role of interleukin-5 (IL-5) and eosinophils in controlling the
development of L. sigmodontis after vaccination and in primary infection. BALB/c mice immunized with irradiated third-stage larvae (L3) were confirmed
to have elevated IL-5 levels as well as high subcutaneous eosinophilia and to attack and reduce incoming larvae within the
first 2 days, resulting in 70% reduction of worm load. Treatment of vaccinated mice with anti-IL-5 antibody (TRFK-5) suppressed
both blood and tissue eosinophilia and completely abolished protection. This demonstrates, for the first time in a fully permissive
filarial infection, that IL-5 is essential for protection induced by irradiated L3 larvae. In contrast, in primary-infected
mice, anti-IL-5 treatment did not modify filarial infection within the 1st month, most likely because during primary infection
IL-5-dependent mechanisms such as subcutaneous eosinophilia are induced too late to disturb worm establishment. However, there
is a role for IL-5 late in primary infection where neutrophil-dependent worm encapsulation is also under the control of IL-5.
Received: 30 March 2000 相似文献
5.
Giulia Barcia Marlne Rio Zahra Assouline Coralie Zangarelli Charles-Joris Roux Pascale de Lonlay Julie Steffann Isabelle Desguerre Arnold Munnich Jean-Paul Bonnefont Nathalie Boddaert Agns Rtig Metodi D. Metodiev Benedetta Ruzzenente 《European journal of human genetics : EJHG》2021,29(3):533
Mitochondrial translation is essential for the biogenesis of the mitochondrial oxidative phosphorylation system (OXPHOS) that synthesizes the bulk of ATP for the cell. Hypomorphic and loss-of-function variants in either mitochondrial DNA or in nuclear genes that encode mitochondrial translation factors can result in impaired OXPHOS biogenesis and mitochondrial diseases with variable clinical presentations. Compound heterozygous or homozygous missense and frameshift variants in the FARS2 gene, that encodes the mitochondrial phenylalanyl-tRNA synthetase, are commonly linked to either early-onset epileptic mitochondrial encephalopathy or spastic paraplegia. Here, we expand the genetic spectrum of FARS2-linked disease with three patients carrying novel compound heterozygous variants in the FARS2 gene and presenting with spastic tetraparesis, axial hypotonia and myoclonic epilepsy in two cases.Subject terms: Metabolic disorders, Mutation 相似文献
6.
Anselme Millogo Ahmed Loukil Coralie LOllivier Diakourga Arthur Djibougou Sylvain Godreuil Michel Drancourt 《Emerging infectious diseases》2021,27(6):1758
Mycobacterium leprae was detected by optical microscopy, fluorescent in situ hybridization, and molecular detection in feces collected for the diagnosis of Entamoeba coli enteritis in a leprosy patient in Burkina Faso. This observation raises questions about the role of fecal excretion of M. leprae in the natural history and diagnosis of leprosy. 相似文献
7.
Julie Frre Olga Chatzis Kelly Cremer Joanna Merckx Mathilde De Keukeleire Florence Renard Nathalie Ribesse Frdric Minner Jean Ruelle Benoit Kabamba Hector Rodriguez-Villalobos Bertrand Bearzatto Marie-Luce Delforge Coralie Henin Fabrice Bureau Laurent Gillet Annie Robert Dimitri Van der Linden 《Viruses》2022,14(10)
Schools have been a point of attention during the pandemic, and their closure one of the mitigating measures taken. A better understanding of the dynamics of the transmission of SARS-CoV-2 in elementary education is essential to advise decisionmakers. We conducted an uncontrolled non-interventional prospective study in Belgian French-speaking schools to describe the role of attending asymptomatic children and school staff in the spread of COVID-19 and to estimate the transmission to others. Each participant from selected schools was tested for SARS-CoV-2 using a polymerase chain reaction (PCR) analysis on saliva sample, on a weekly basis, during six consecutive visits. In accordance with recommendations in force at the time, symptomatic individuals were excluded from school, but per the study protocol, being that participants were blinded to PCR results, asymptomatic participants were maintained at school. Among 11 selected schools, 932 pupils and 242 school staff were included between January and May 2021. Overall, 6449 saliva samples were collected, of which 44 came back positive. Most positive samples came from isolated cases. We observed that asymptomatic positive children remaining at school did not lead to increasing numbers of cases or clusters. However, we conducted our study during a period of low prevalence in Belgium. It would be interesting to conduct the same analysis during a high prevalence period. 相似文献
8.
Sandra Lacote Carole Tamietti Mehdi Chabert Marie-Pierre Confort Laurine Conquet Coralie Pulido Nomie Aurine Camille Baquerre Adrien Thiesson Bertrand Pain Marcelo De Las Heras Marie Flamand Xavier Montagutelli Philippe Marianneau Maxime Ratinier Frdrick Arnaud 《Viruses》2022,14(11)
Rift Valley fever virus (RVFV) is a pathogenic arthropod-borne virus that can cause serious illness in both ruminants and humans. The virus can be transmitted by an arthropod bite or contact with contaminated fluids or tissues. Two live-attenuated veterinary vaccines—the Smithburn (SB) and Clone 13 (Cl.13)—are currently used during epizootic events in Africa. However, their residual pathogenicity (i.e., SB) or potential of reversion (i.e., Cl.13) causes important adverse effects, strongly limiting their use in the field. In this study, we infected immunocompetent mice with SB or Cl.13 by a subcutaneous or an intranasal inoculation. Interestingly, we found that, unlike the subcutaneous infection, the intranasal inoculation led to a high mortality rate. In addition, we detected high titers and viral N antigen levels in the brain of both the SB- and Cl.13-infected mice. Overall, we unveil a clear correlation between the pathogenicity and the route of administration of both SB and Cl.13, with the intranasal inoculation leading to a stronger neurovirulence and higher mortality rate than the subcutaneous infection. 相似文献
9.