SiO2刺激矽肺肺泡巨噬细胞对MMP-9和TIMP-1表达的影响

Objective To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of metalloproteinase-1 (TIMP-1)in human silicofic alveolar macrophages (AM).Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3h,6h,12h,18h,24h and 36h.The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.Results The expressions of MMP-9 in the AM increased clearly along with the time,reached peak at 24 h when detected with zymography (average optical density:3.061±0.153 vs 2.851±0.164,P＜0.05);and reached peak at 18h when detected with immunological method (average optical density:0.386±0.037 vs 0.322±0.034,P＜0.05).The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P＞0.05).Conclusion SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM.     

唐山市苯丙酮尿症患儿筛查及PAH基因突变情况分析

摘要：目的　分析唐山市苯丙酮尿症（PKU）患儿筛查结果及苯丙氨酸羟化酶（PAH）基因突变的情况。方法　选取2015年1月—2018年12月唐山市新生儿303 777例,通过茚三酮免疫荧光法检测新生儿足跟血中苯丙氨酸（PA）含量。再利用聚合酶链反应（PCR）和基因测序的方法对筛查出的PKU患儿PAH基因进行检测。结果　303 777例新生儿初步筛查共发现609例可疑阳性,召回其中411例（67.49%）进行复查,确诊42例（13.8/10万）。42例PKU患者的PAH基因测序显示,在84条染色体上共检测到62个（73.81%）12种突变,其中错义突变8种,无义突变2种,缺失突变1种,剪接突变1种。患者PAH基因突变分布在第2、3、6、7、9外显子上,其中第7外显子最多（35个,56.45%）,其次为第3外显子（14个,22.58%）。最常见的突变基因为Exon7-R243Q（18个,29.03%）和Exon3-R111X（10个,16.13%）、Exon7-R261Q（10个,16.13%）。筛查中发现1例典型PKU患儿,该患儿在PAH基因外显子区域同时发现2处杂合突变：c.208-210delTCT（缺失突变）和c.964G＞A（鸟嘌呤＞腺嘌呤）。结论　唐山市新生儿PKU发病率略高于全国,PAH基因突变以错义突变为主,第7外显子是唐山市患儿PAH基因高频突变位点。     

姜黄素对矽肺大鼠肺组织PDGF及ERK1/2表达的影响

将雄性Wistar大鼠40只,随机分为正常对照组、生理氯化钠溶液组、模型组、溶剂对照组、姜黄素组。采用免疫印迹法、实时荧光定量聚合酶链反应法检测各组大鼠肺组织的血小板源性生长因子(PDGF)和磷酸化细胞外信号调节激酶1/2(p-ERK1/2)蛋白及mRNA表达。结果显示,矽肺模型组、溶剂对照组大鼠肺组织中PDGF、p-ERK1/2蛋白及mRNA表达较正常对照组和生理氯化钠溶液组增高(P0.05),姜黄素组大鼠肺组织PDGF、p-ERK1/2蛋白及mRNA表达较模型组降低(P0.05)。提示姜黄素可降低矽肺模型组大鼠肺组织中PDGF、p-ERK1/2蛋白及mRNA表达,并可能通过影响PDGF、p-ERK1/2的表达在矽肺发病中起到一定的保护作用。     

尖端扭转型室速五例综合报告并文献复习

尖端扭转型室速(Torsade de pointes,下简单TDP)并非罕见,国内外文献屡有报道。本文就当阳县人民医院陈宗华、武警湖北总队医院郭志义,武汉市职工医学院附属医院杨延祥,湖北咸宁195医院向培斌三篇报告共5例TDP综合报告如下,并结合文献作一复习。     

结直肠癌合并腺瘤的处理

结直肠癌合并腺瘤者极为常见，临床上往往重视结直肠癌而忽视或遗漏了对腺瘤的处理，是影响结直肠癌术后疗效及结直肠重复癌发生率高的重要因素之一。我院１９９５年１月至２０００年１２月期间共收治结直肠癌合并腺瘤患者１３２例，占同期结直肠癌的１８％，分析如下。临床资料本组患者男７５例、女５７例，平均年龄６２岁。腺瘤分布于直肠５７例（４３．２％），乙状结肠２４例（１８．２％），其它部位５１例（３８．６％）。腺瘤单发７２例（５４．５％），多发６０例（４５．５％）。腺瘤直径小于１．０cm８０例（６０．６％），１～２…     

生血宁片治疗缺铁性贫血的临床观察

临床观察60例缺铁性贫血（IDA）患者使用生血宁片前后血清铁、总铁结合力：血清铁蛋白、血红蛋白含量、平均红细胞容积、平均红细胞血红蛋白含量、平均红细胞血红蛋白浓度的变化，经统计学处理，各项指标治疗前后存在显著性差异（P〈0．01）。且患者的症状得到明显改善。愈显率78．3％，总有效率100％。     

HBV感染综合标志物特征性改变对HCC的诊断价值

目的 探讨HBV感染综合乙肝标志物(CHBM)特征性改变在提高肝细胞癌(HCC)早期诊断灵敏度中的作用。方法 选择2016年1月—2022年5月在华北理工大学附属医院确诊的患者为研究对象,共992例,其中慢性乙型肝炎组236例,肝硬化组202例,HCC组265例,健康对照组289例,分别检测四组中甲胎蛋白(AFP)、癌胚抗原(CEA)、糖类抗原199(CA199)、碱性磷酸酶(ALP)、γ-谷氨酰转移酶(GGT)、总胆汁酸(TBA)、乙型肝炎表面抗原(HBs Ag)、乙型肝炎e抗原(HBe Ag)、乙型肝炎e抗体(抗-HBe)、HBV DNA的水平及阳性率,寻找慢性乙型肝炎转归为HCC时综合乙肝标志物水平及阳性率变化趋势,并将此变化趋势视为“综合乙肝标志物阳性”。将此综合标志物与传统指标联合检测,对数据进行统计分析。结果 在合并HBV感染的HCC组中,血清HBs Ag滴度≤1000IU/ml者占61.82%,HBV DNA≤10000copies/ml者占61.41%,HBe Ag阳性者占20.33%,抗-HBe阳性者占67.22%。“AFP+GGT+综合乙肝标志物”和“AFP+CEA...     

普理灵疝装置在老年复发性腹股沟疝中的应用

目的：探讨普理灵疝装置（PHS）在老年复发性腹股沟疝中的临床应用价值。方法：回顾性分析应用普理灵疝装置治疗老年复发性腹股沟疝病人28例的临床资料。结果：28例手术均成功,平均手术时间50min,随访2-30个月,无复发病例。结论：普理灵疝装置无张力疝修补术是治疗老年腹股沟复发疝的理想术式,增加可靠性,降低手术难度,创伤小,恢复快,复发率低,特别注意老年人围手术期的处理和“个体化”。     

SiO2刺激矽肺肺泡巨噬细胞对MMP-9和TIMP-1表达的影响

Objective To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of metalloproteinase-1 (TIMP-1)in human silicofic alveolar macrophages (AM).Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3h,6h,12h,18h,24h and 36h.The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.Results The expressions of MMP-9 in the AM increased clearly along with the time,reached peak at 24 h when detected with zymography (average optical density:3.061±0.153 vs 2.851±0.164,P＜0.05);and reached peak at 18h when detected with immunological method (average optical density:0.386±0.037 vs 0.322±0.034,P＜0.05).The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P＞0.05).Conclusion SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM.     

SiO2刺激矽肺肺泡巨噬细胞对MMP-9和TIMP-1表达的影响

Objective To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of metalloproteinase-1 (TIMP-1)in human silicofic alveolar macrophages (AM).Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3h,6h,12h,18h,24h and 36h.The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.Results The expressions of MMP-9 in the AM increased clearly along with the time,reached peak at 24 h when detected with zymography (average optical density:3.061±0.153 vs 2.851±0.164,P＜0.05);and reached peak at 18h when detected with immunological method (average optical density:0.386±0.037 vs 0.322±0.034,P＜0.05).The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P＞0.05).Conclusion SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM.