结直肠癌组织中Thr187磷酸化p27kip1的表达及临床意义

目的 探讨第187位苏氨酸（Thr187）磷酸化的细胞周期素依赖性激酶抑制蛋白（p27kip1,简称p27）（P-p27Thr187）在结直肠癌发生、发展中的作用及其与结直肠癌临床病理特征的关系。方法 收集行手术切除的80例结直肠癌组织标本,以及6对新鲜结直肠癌和相应癌旁正常组织标本;采用免疫组织化学EnVision二步法和蛋白质印迹法检测结直肠癌组织及相应癌旁正常组织中P-p27Thr187和p27的表达;并分析其与结直肠癌临床病理特征的关系。结果免疫组织化学和蛋白质印迹结果均显示,P-p27Thr187在结直肠癌组织中的阳性表达率明显高于癌旁正常组织;结直肠癌组织中P-p27Thr187阳性表达率为100.0%（80/80）,高表达者52例（65.0%,52/80）,低表达者28例（35.0%,28/80）;而癌旁正常组织中P-p27Thr187阳性表达率为13.8%（11/80）,与结直肠癌组织比较差异有统计学意义（P＜0.05）;p27在结直肠癌组织中的阳性表达率为48.8%（39/80）,癌旁正常组织中为90.0%（72/80）,两组比较差异有统计学意义（P＜0.05）;P-p27Thr187阳性表达与肿瘤细胞分化程度和TNM分期有关（均P＜0.05）;P-p27Thr187表达强度与细胞增殖指标Ki-67表达强度呈正相关（r=0.487,P＜0.05）,与p27表达强度呈负相关（r=-0.394,P＜0.05）。结论结直肠癌组织中P-p27Thr187的异常表达可能参与了结直肠癌的发生、发展过程,其有助于综合评估结直肠癌的生物学行为,对结直肠癌的预后判断及诊治具有一定的参考价值。     

炎性疼痛引起脊髓后角ERK的表达变化及其年龄差异

目的 探讨疼痛模型大鼠脊髓后角的细胞外调节因子(ERK)表达变化及其年龄差异.方法 应用免疫组织化学方法检测ERK在脊髓后角的定位表达,并用图像分析系统和免疫印迹法进行定量分析.结果 免疫组化显示正常对照组,不同年龄组大鼠脊髓后角ERK阳性反应无明显差异;福尔马林刺激后,中年组、青年组大鼠刺激侧脊髓后角ERK阳性反应均有明显增加,但老年组大鼠ERK阳性反应则无明显变化.结论 疼痛刺激可以上调脊髓后角ERK的表达,但这种上调因年龄不同而存在差异.     

雌、孕激素受体和Her-2阴性乳腺癌的临床病理特征

Objective To study the clinicopathologic characteristics of triple-negative breast cancer (TNBC) and its value in the prediction of prognosis. Method In this study,500 cases of female breast cancers were examined immunohistochcmically for the TNBC. The clinicopathologic characteristics of the 243 TNBC cases were inspected. Results TNBC accounted for 17.6% (88/500) of the 500 breast cancers. The histological types of the TNBC included mainly infihrative ductal carcinoma, metaplastic carcinoma and medullar carcinoma. Among those, histological grade Ⅲ accounted for 72.7% (64/88) of all the TNBC and was more common than that in hormone receptor positive breast cancers (HR+ group ) and Her-2 overexpression breast cancers (Her-2 group)(P=0.000). The positive rates of CK5/6 and EGFR in the TNBC were 30.7% (27/88) and 34.1% (30/88), respectively. The positive rates of ERCC1 and KIT in the TNBC were 28.4% (25/88) and 34.1% (30/88), respectively, Both of which were higher than those in the HR + group and Her-2 group, respectively (P=0.032 and P=0.026). 3-year survival rate of the TNBC was 71.5% and it was lower than that of HR group (P=0.021) and not significantly different from that of Her-2 group (P=0.474). Conclusions TNBC is the breast cancer with high aggressive pathologic futures and poor prognosis. EGFR and ERCC1 expression were positive in a portion of TNBC cases.     

乳腺导管原位癌三级淋巴结构的临床病理特征分析及其与肿瘤进展的关系

目的 探讨乳腺导管原位癌（ductal carcinoma in situ, DCIS）三级淋巴结构（tertiary lymphoid structures, TLS）的临床病理特征及其在DCIS浸润和进展中的意义。方法 收集103例DCIS、32例乳腺导管原位癌伴微浸润（ductal carcinoma in situ with microinvasion, DCIS-MI）和78例浸润性导管癌（infiltrating ductal carcinoma, IDC）相关临床病理资料,采用HE和免疫组化法分别观察DCIS、DCIS-MI和IDC癌灶内TLS密度,评价TLS与临床病理特征的关系。结果 在DCIS中不同核级组间TLS密度差异有统计学意义（P<0.001）,且随着核级的增高TLS密度增高。DCIS-MI的TLS发生率为96.9%（31/32）,高于DCIS的TLS发生率[78.6%（81/103）]（χ²=21.680,P<0.001）;不同核级组间TLS密度差异有统计学意义（P=0.007）。IDC患者组织学分级组间（P<0.001...     

胸膜恶性纤维组织细胞瘤一例并文献复习

胸膜原发恶性纤维组织细胞瘤是一种罕见肿瘤,本文报告了1例我院收治的胸膜原发恶性纤维组织细胞瘤患者,并进行国内外相关文献复习,探讨其诊断及治疗方式。     

雌、孕激素受体和Her-2阴性乳腺癌的临床病理特征

目的 评价雌、孕激素受体和Her-2表达均阴性乳腺癌(triple-negative breast cancer,TNBC)的临床病理特征及其对预后的影响.方法 500例乳腺癌采用免疫组化筛选TNBC,观察TNBC的临床病理特征,并对其中243例乳腺癌进行临床随访. 结果 500例乳腺癌中TNBC占17.6%(88/500).组织学类型主要为浸润性导管癌(NOS)、化生性癌和髓样癌.组织分级Ⅲ级占72.7%(64/88),高于激素受体阳性组和Her-2高表达组(P=0.000).TNBC中CK5/6阳性率30.7%(27/88),EGFR阳性率34.1%(30/88).TNBC中ERCC1和KIT阳性率分别为28.4%(25/88)和34.1%(30/88),均分别高于激素受体阳性组和Her-2高表达组(P=0.032和P=0.026).TNBC 3年累积生存率为71.5%,低于激素受体阳性组(P=0.021),与Her-2高表达组差异无统计学意义(P=0.474). 结论 TNBC是一类具有高侵袭性病理特征和预后不良的乳腺癌;部分病例表达EGFR和ERCC1.     

结直肠癌组织Tip60蛋白表达及其临床意义探讨

目的:探讨结直肠癌组织中Tip60蛋白的表达及其临床意义。方法:选择2010-02-01-2011-02-01嘉兴市第一医院行手术治疗的结直肠癌患者100例和结直肠腺瘤患者20例,采用免疫组织化学检测Tip60蛋白在100例结直肠癌组织和相应癌旁正常组织(距离癌组织>3cm)及20例结直肠腺瘤组织中的表达及差异,观察Tip60和Ki-67及Bcl-2蛋白在结直肠癌组织中表达的相关性。结果:Tip60蛋白在结直肠癌组织中的阳性表达率为24%(24/100),癌旁正常组织为72%(72/100),结直肠腺瘤组织为75%(15/20),结直肠癌中的表达明显低于癌旁组织,χ2=46.154,P<0.001;也低于腺瘤组织,χ2=19.761,P<0.001;癌旁组织及腺瘤组织的表达差异无统计学意义,χ2=0.075,P=0.781。Tip60的表达与患者性别(χ2=1.629,P=0.202)、年龄(χ2=1.170,P=0.279)、肿瘤浸润深度(χ2=3.102,P=0.078)及淋巴结转移(χ2=0.718,P=0.397)无关,而与肿瘤大小(χ2=14.649,P<0.001)、肿瘤分化程度(χ2=6.469,P=0.039)和Duke分期(χ2=4.702,P=0.030)相关。Tip60和Ki-67的表达呈负相关,r=-0.273,P=0.006,Tip60和Bcl-2蛋白的表达呈负相关,r=-0.216,P=0.031。Logistic回归分析发现,肿瘤大小是结直肠癌Tip60蛋白表达的独立相关因素,B=-1.833,P<0.001。结论:Tip60蛋白在结直肠癌组织中表达下调,且与结直肠癌的进展有关,Tip60可作为预测结直肠癌进展和预后的生物学指标。     

USP22、FoxM1在结直肠癌中的表达研究

目的探讨去泛素酶基因（USP22）和叉头框M1基因（FoxM1）表达在结直肠癌发生、发展中的作用,以及与患者临床病理特征的关系。方法选取行手术切除的结直肠癌组织标本120例和正常结直肠黏膜组织标本32例;采用免疫组织化学EnVision二步法和Westernblot检测结直肠癌组织及相应癌旁正常组织中USP22、FoxM1及Wnt/β-catenin通路相关蛋白（β-catenin、LEF/TCF、c-myc）的表达。结果结直肠癌组织中USP22、FoxM1的阳性表达率均明显高于癌旁正常组织（均P＜0.05）。结直肠癌组织中USP22、FoxM1、β-catenin、LEF/TCF、c-myc蛋白的表达水平均明显高于癌旁正常组织（均P＜0.05）。结直肠癌组织中USP22、FoxM1表达与肿瘤分化程度、淋巴结转移、肝脏转移及Duke''s分期均有关（均P＜0.05）;且USP22/FoxM1共表达与肿瘤淋巴结转移、肝脏转移及Duke''s分期亦均有关（均P＜0.05）。结论结直肠癌组织中USP22、FoxM1的异常高表达可能参与了结直肠癌的发生、发展过程,且两者具有协同作用。     

δ-连环素在雌激素替代治疗大鼠小脑中的表达

目的 探讨卵巢切除大鼠小脑中δ-连环素(δ-catenin)的表达特征及雌激素对其表达的影响.方法 将大鼠分为正常组、假手术组、雌激素替代组和安慰剂组.卵巢切除后人工注射雌二醇或安慰剂,应用免疫组织化学方法检测δ-连环素在小脑的定位表达;用免疫印迹法做定量分析.结果 与假手术组和雌激素替代组相比,免疫组织化学和免疫印迹法均显示安慰剂组大鼠小脑中δ-连环素表达最低,假手术组和雌激素替代组δ-连环素表达明显高于安慰剂组.结论 雌激素可上调卵巢切除大鼠小脑δ-连环素的表达.     

雌、孕激素受体和Her-2阴性乳腺癌的临床病理特征

Objective To study the clinicopathologic characteristics of triple-negative breast cancer (TNBC) and its value in the prediction of prognosis. Method In this study,500 cases of female breast cancers were examined immunohistochcmically for the TNBC. The clinicopathologic characteristics of the 243 TNBC cases were inspected. Results TNBC accounted for 17.6% (88/500) of the 500 breast cancers. The histological types of the TNBC included mainly infihrative ductal carcinoma, metaplastic carcinoma and medullar carcinoma. Among those, histological grade Ⅲ accounted for 72.7% (64/88) of all the TNBC and was more common than that in hormone receptor positive breast cancers (HR+ group ) and Her-2 overexpression breast cancers (Her-2 group)(P=0.000). The positive rates of CK5/6 and EGFR in the TNBC were 30.7% (27/88) and 34.1% (30/88), respectively. The positive rates of ERCC1 and KIT in the TNBC were 28.4% (25/88) and 34.1% (30/88), respectively, Both of which were higher than those in the HR + group and Her-2 group, respectively (P=0.032 and P=0.026). 3-year survival rate of the TNBC was 71.5% and it was lower than that of HR group (P=0.021) and not significantly different from that of Her-2 group (P=0.474). Conclusions TNBC is the breast cancer with high aggressive pathologic futures and poor prognosis. EGFR and ERCC1 expression were positive in a portion of TNBC cases.