金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

Objective To study the proteomic change in Iymphocytes of rabbits with scald injury and Staphylococcus aureus (SA) invasion. Methods Twenty-four rabbits were divided into four groups as follows: control group, scald group, scald with SA invasion 2 hs group, and scald with SA invasion 6 hs group, according to random number table, with 6 rabbits in each group. Except for rabbits in control group (sham scald at 37 ℃ ) , rabbits in the other 3 groups were subjected to 30% TBSA full-thickness scald. Rabbits in SA invasion 2 and 6 hs groups were injected with 2 mL (1.0×108 CFU/mL) SA suspension, which was in the log growth phase, via auricle vein 18 hs and 22 hs after injury. Whole blood samples were collected from carotid artery of rabbits in 4 groups 24 hs after scald. Lymphocytes were isolated and its ex-tracted proteins were analyzed by two-dimensional gel electrophoresis coupled with mass spectroscopy. Re-suits About 1030 protein spots of lymphocytes were detected in each group. Compared with that of control group, 19 protein spots were found to be differentially expressed in the other 3 groups, and 11 spots (10 pro-teins) were identified. Expression levels of cofilin, cyclophilin A, ubiquitin, nucleoside diphosphate ki-nase, glutamate dehydrogenase and annexin 1 were down-regulated, but expression level of peroxiredoxin was up-regulated obviously. Conclusions There is obvious proteornic change in lymphocytes of scalded rabbits or of scalded rabbits invaded by SA, and it may relate to immune suppression and sepsis after injury.     

金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

Objective To study the proteomic change in Iymphocytes of rabbits with scald injury and Staphylococcus aureus (SA) invasion. Methods Twenty-four rabbits were divided into four groups as follows: control group, scald group, scald with SA invasion 2 hs group, and scald with SA invasion 6 hs group, according to random number table, with 6 rabbits in each group. Except for rabbits in control group (sham scald at 37 ℃ ) , rabbits in the other 3 groups were subjected to 30% TBSA full-thickness scald. Rabbits in SA invasion 2 and 6 hs groups were injected with 2 mL (1.0×108 CFU/mL) SA suspension, which was in the log growth phase, via auricle vein 18 hs and 22 hs after injury. Whole blood samples were collected from carotid artery of rabbits in 4 groups 24 hs after scald. Lymphocytes were isolated and its ex-tracted proteins were analyzed by two-dimensional gel electrophoresis coupled with mass spectroscopy. Re-suits About 1030 protein spots of lymphocytes were detected in each group. Compared with that of control group, 19 protein spots were found to be differentially expressed in the other 3 groups, and 11 spots (10 pro-teins) were identified. Expression levels of cofilin, cyclophilin A, ubiquitin, nucleoside diphosphate ki-nase, glutamate dehydrogenase and annexin 1 were down-regulated, but expression level of peroxiredoxin was up-regulated obviously. Conclusions There is obvious proteornic change in lymphocytes of scalded rabbits or of scalded rabbits invaded by SA, and it may relate to immune suppression and sepsis after injury.     

金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

Objective To study the proteomic change in Iymphocytes of rabbits with scald injury and Staphylococcus aureus (SA) invasion. Methods Twenty-four rabbits were divided into four groups as follows: control group, scald group, scald with SA invasion 2 hs group, and scald with SA invasion 6 hs group, according to random number table, with 6 rabbits in each group. Except for rabbits in control group (sham scald at 37 ℃ ) , rabbits in the other 3 groups were subjected to 30% TBSA full-thickness scald. Rabbits in SA invasion 2 and 6 hs groups were injected with 2 mL (1.0×108 CFU/mL) SA suspension, which was in the log growth phase, via auricle vein 18 hs and 22 hs after injury. Whole blood samples were collected from carotid artery of rabbits in 4 groups 24 hs after scald. Lymphocytes were isolated and its ex-tracted proteins were analyzed by two-dimensional gel electrophoresis coupled with mass spectroscopy. Re-suits About 1030 protein spots of lymphocytes were detected in each group. Compared with that of control group, 19 protein spots were found to be differentially expressed in the other 3 groups, and 11 spots (10 pro-teins) were identified. Expression levels of cofilin, cyclophilin A, ubiquitin, nucleoside diphosphate ki-nase, glutamate dehydrogenase and annexin 1 were down-regulated, but expression level of peroxiredoxin was up-regulated obviously. Conclusions There is obvious proteornic change in lymphocytes of scalded rabbits or of scalded rabbits invaded by SA, and it may relate to immune suppression and sepsis after injury.     

金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

Objective To study the proteomic change in Iymphocytes of rabbits with scald injury and Staphylococcus aureus (SA) invasion. Methods Twenty-four rabbits were divided into four groups as follows: control group, scald group, scald with SA invasion 2 hs group, and scald with SA invasion 6 hs group, according to random number table, with 6 rabbits in each group. Except for rabbits in control group (sham scald at 37 ℃ ) , rabbits in the other 3 groups were subjected to 30% TBSA full-thickness scald. Rabbits in SA invasion 2 and 6 hs groups were injected with 2 mL (1.0×108 CFU/mL) SA suspension, which was in the log growth phase, via auricle vein 18 hs and 22 hs after injury. Whole blood samples were collected from carotid artery of rabbits in 4 groups 24 hs after scald. Lymphocytes were isolated and its ex-tracted proteins were analyzed by two-dimensional gel electrophoresis coupled with mass spectroscopy. Re-suits About 1030 protein spots of lymphocytes were detected in each group. Compared with that of control group, 19 protein spots were found to be differentially expressed in the other 3 groups, and 11 spots (10 pro-teins) were identified. Expression levels of cofilin, cyclophilin A, ubiquitin, nucleoside diphosphate ki-nase, glutamate dehydrogenase and annexin 1 were down-regulated, but expression level of peroxiredoxin was up-regulated obviously. Conclusions There is obvious proteornic change in lymphocytes of scalded rabbits or of scalded rabbits invaded by SA, and it may relate to immune suppression and sepsis after injury.     

地塞米松对烧伤早期中性粒细胞体外凋亡的影响

目的观察地塞米松对烧伤早期中性粒细胞体外凋亡的影响及bcl-2、核因子кB(NFкB)蛋白表达的变化。方法应用荧光染色和流式细胞仪观察地塞米松对正常血清和烧伤血清诱导烧伤早期中性粒细胞体外自发凋亡的影响;免疫组织化学SP法检测中性粒细胞bcl-2、NFкB蛋白表达。结果烧伤血清较正常血清诱导中性粒细胞凋亡减少(6.18±0.96vs.21.77±2.32,P<0.05),bcl-2、NFкB蛋白表达增加(83.27±5.45vs.49.95±2.67,0.1802±0.0130vs.0.0434±0.0196,P<0.05);加入地塞米松后,烧伤血清组凋亡增加(12.67±0.71vs.6.18±0.96,P<0.05);且NFкB蛋白表达减少(0.1031±0.0154vs.0.1802±0.0130,P<0.05),而正常血清组差异无显著性。结论地塞米松可减轻烧伤血清对中性粒细胞凋亡的抑制作用,其机制可能与NFкB蛋白表达下调有关。     

金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

Objective To study the proteomic change in Iymphocytes of rabbits with scald injury and Staphylococcus aureus (SA) invasion. Methods Twenty-four rabbits were divided into four groups as follows: control group, scald group, scald with SA invasion 2 hs group, and scald with SA invasion 6 hs group, according to random number table, with 6 rabbits in each group. Except for rabbits in control group (sham scald at 37 ℃ ) , rabbits in the other 3 groups were subjected to 30% TBSA full-thickness scald. Rabbits in SA invasion 2 and 6 hs groups were injected with 2 mL (1.0×108 CFU/mL) SA suspension, which was in the log growth phase, via auricle vein 18 hs and 22 hs after injury. Whole blood samples were collected from carotid artery of rabbits in 4 groups 24 hs after scald. Lymphocytes were isolated and its ex-tracted proteins were analyzed by two-dimensional gel electrophoresis coupled with mass spectroscopy. Re-suits About 1030 protein spots of lymphocytes were detected in each group. Compared with that of control group, 19 protein spots were found to be differentially expressed in the other 3 groups, and 11 spots (10 pro-teins) were identified. Expression levels of cofilin, cyclophilin A, ubiquitin, nucleoside diphosphate ki-nase, glutamate dehydrogenase and annexin 1 were down-regulated, but expression level of peroxiredoxin was up-regulated obviously. Conclusions There is obvious proteornic change in lymphocytes of scalded rabbits or of scalded rabbits invaded by SA, and it may relate to immune suppression and sepsis after injury.     

金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

Objective To study the proteomic change in Iymphocytes of rabbits with scald injury and Staphylococcus aureus (SA) invasion. Methods Twenty-four rabbits were divided into four groups as follows: control group, scald group, scald with SA invasion 2 hs group, and scald with SA invasion 6 hs group, according to random number table, with 6 rabbits in each group. Except for rabbits in control group (sham scald at 37 ℃ ) , rabbits in the other 3 groups were subjected to 30% TBSA full-thickness scald. Rabbits in SA invasion 2 and 6 hs groups were injected with 2 mL (1.0×108 CFU/mL) SA suspension, which was in the log growth phase, via auricle vein 18 hs and 22 hs after injury. Whole blood samples were collected from carotid artery of rabbits in 4 groups 24 hs after scald. Lymphocytes were isolated and its ex-tracted proteins were analyzed by two-dimensional gel electrophoresis coupled with mass spectroscopy. Re-suits About 1030 protein spots of lymphocytes were detected in each group. Compared with that of control group, 19 protein spots were found to be differentially expressed in the other 3 groups, and 11 spots (10 pro-teins) were identified. Expression levels of cofilin, cyclophilin A, ubiquitin, nucleoside diphosphate ki-nase, glutamate dehydrogenase and annexin 1 were down-regulated, but expression level of peroxiredoxin was up-regulated obviously. Conclusions There is obvious proteornic change in lymphocytes of scalded rabbits or of scalded rabbits invaded by SA, and it may relate to immune suppression and sepsis after injury.     

金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

目的 观察金黄色葡萄球菌(SA)侵袭烫伤家兔后淋巴细胞蛋白质组的变化.方法 将24只家兔按随机数字表法分为对照组、烫伤组、sA侵袭2 h组、sA侵袭6 h组,每组6只.对照组家兔37℃模拟烫伤;其余3组家兔均造成背部30%TBSAⅢ度烫伤,SA侵袭2、6 h组分别于伤后18、22 h经耳缘静脉注射2 mL(1.0×108CFU/mL)对数生长期sA悬液.4组家兔致伤后24 h取颈动脉血分离获取淋巴细胞,提取细胞总蛋白,进行双向凝胶电泳和质谱分析.结果 4组家兔淋巴细胞平均蛋向质点数约为1030个;与对照组比较,其余3组筛选出差异蛋白质点19个,1鉴定了其中11个蛋白质点,包括10种蛋白质.表达下调的蛋白质有丝切蛋白、胞溶质蛋白A、泛素、核苷二磷酸激酶、,奋氨酸脱氢酶和膜联蛋白1,而过氧化物氧化还原素表达明显上调.结论 家兔烫伤后或烫伤+SA侵袭后淋巴细胞蛋白质组发生了明显变化,这可能与伤后免疫抑制和脓毒症的发生有关.     

游离背阔肌肌瓣充填修复半侧颜面萎缩

目的探讨游离背阔肌肌瓣充填修复半侧颜面萎缩的效果。方法术前石膏倒模，根据石膏模型设计右侧游离背阔肌肌瓣并进行移植填充，矫正半侧颜面萎缩。肌瓣上于蒂部旁携带1cm×2cm大小皮肤，缝于下颌角前缘切口，以便术后观察血运。胸背动、静脉分别与面动脉、面前静脉吻合，术后常规抗血栓、抗痉挛、抗感染等治疗。结果两例肌皮瓣均成活。术后下颌（即蒂部）稍显臃肿，一例鼻唇沟填塞组织稍有外移、下坠，3个月后再次手术修复后效果满意。结论准确的组织瓣设计和塑形、可靠的血管显微吻合、良好的组织瓣固定是游离组织瓣充填修复半侧颜面萎缩手术成功的三个关键．     

金黄色葡萄球菌侵袭烫伤家兔致淋巴细胞蛋白质组的变化

Objective To study the proteomic change in Iymphocytes of rabbits with scald injury and Staphylococcus aureus (SA) invasion. Methods Twenty-four rabbits were divided into four groups as follows: control group, scald group, scald with SA invasion 2 hs group, and scald with SA invasion 6 hs group, according to random number table, with 6 rabbits in each group. Except for rabbits in control group (sham scald at 37 ℃ ) , rabbits in the other 3 groups were subjected to 30% TBSA full-thickness scald. Rabbits in SA invasion 2 and 6 hs groups were injected with 2 mL (1.0×108 CFU/mL) SA suspension, which was in the log growth phase, via auricle vein 18 hs and 22 hs after injury. Whole blood samples were collected from carotid artery of rabbits in 4 groups 24 hs after scald. Lymphocytes were isolated and its ex-tracted proteins were analyzed by two-dimensional gel electrophoresis coupled with mass spectroscopy. Re-suits About 1030 protein spots of lymphocytes were detected in each group. Compared with that of control group, 19 protein spots were found to be differentially expressed in the other 3 groups, and 11 spots (10 pro-teins) were identified. Expression levels of cofilin, cyclophilin A, ubiquitin, nucleoside diphosphate ki-nase, glutamate dehydrogenase and annexin 1 were down-regulated, but expression level of peroxiredoxin was up-regulated obviously. Conclusions There is obvious proteornic change in lymphocytes of scalded rabbits or of scalded rabbits invaded by SA, and it may relate to immune suppression and sepsis after injury.