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急性痢疾多见于夏秋季,其它季节也时有发生。笔者用碘酊红糖液内服治疗急性痢疾112例,取得了较满意的效果,现报告如下。一般资料112例中男性62例,女性50例;年龄5~9岁24例,10~19岁30例,20~70岁58例;病程均<5日。患者均有发病急骤,... 相似文献
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Objective To design a device based on immunobead method which enables extraction of exfoliated colonocytes from human feces to become an automatic concentrating process. Methods The automatic device for extraction of exfoliated colonocytes from human feces was designed based on the composition and physiochemical features of the feces as well as the distribution of exfoliated colonocytes, so as to integrate its functions in sample collection, pretreatment, exfoliated colonocyte-immunobead binding,and exfoliated colonocyte- immunobead complex washing. The immunobeads were prepared by coating magnetic beads with monoclonal antibody of epethelial cell adhesion molecule (EpCAM) Ber-EP4 and used to concentrate colorectal cancer cells from cell culture. The binding of colorectal cells to immunobeads was observed under microscopy. Cancer tissues of 10 colorectal cancer patients (or tissues around cancer) were collected. The automatic device for extraction of exfoliated colonocytes from human feces was used to get exfoliated cells from stool specimens of the patients. QRT-PCR was used to detect gene expression of c-myc、cox-2、CD44v6 in tissues and cells collected. Rate of gene positive expression of different original specimens was compared. Results The automatic device for extraction of colonocytes from human feces was designed and manufactured. Extracting exfoliated coloncytes from feces sample was successfully completed by the automatic device and self-prepared immunobeads with coating special monoclonal antibody. The recovery rate of cells was 27%. Rate of c-myc, cox-2 and CD44v6 gene positive expression in cancer tissues or tissues around cancer was 20%, 10% and 20% respectively, and 100% in exfoliated cells from stool specimens. The difference of positive rate among specimens was significant (all P<0.01). Exfoliated cells from stool specimens collected by the automatic device for extraction of exfoliated colonocytes from human feces were cancer cells. Conclusions As one of the latest-developed approaches, immunobead-based concentration of exfoliated colonocytes appears practical. An automatic device thereby developed may be used for early detection of colorectal cancers. 相似文献
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目的 :研究一种基于射频识别技术(radio frequency identification,RFID)的卫生装备质量控制管理系统,为急救类战储卫生装备的日常质量控制和仓储管理提供技术支持。方法:通过对战储卫生装备质量控制业务的需求分析,结合RFID技术,采用客户端/服务端(C/S)架构,以My SQL数据库作为服务端,选择Microsoft Visual C++开发语言进行编程。结果:该系统实现了急救类战储卫生装备质量控制和仓储业务信息的快速采集和及时更新,提高了装备的储备效益和管理水平。结论:该系统实用性强,可有效解决装备质量控制分散数据采集的瓶颈问题,实现对战储卫生装备质量控制信息的动态管理。 相似文献
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目的:探讨外固定架治疗股骨粗隆间骨折的临床疗效.方法:应用外固定支架治疗股骨粗隆间骨折156例,术后第2天开始鼓励患者做起、翻身、练习膝、踝关节屈伸功能及股四头肌收缩,2周后可扶拐下床活动.4~12个月后,视骨折愈合情况选择时机拆除外固定架.结果:本组156例获得随访,住院期间无死亡病例发生.术后无脱针、断针、股骨头切割现象.术后髋关节功能恢复良好,优良率97.4%.结论:应用外固定支架治疗股骨粗隆间骨折符合微创理念,体现了有限手术论,手术操作方便简单,固定牢靠,可以早期离床活动,减少长期卧床的并发症,疗效确切. 相似文献
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