不同门奇断流术式对门静脉高压症犬食管下段血管再生的影响

Objective To explore the effect on blood vessel regeneration of distal esophagus in ca-nines of portal hypertension with liver cirrhosis after different procedures with paraesophagastrie devascular-ization. Methods Portal hypertension models were produced in canines by subcutaneous injection of 60% CC14 combined with food restriction. Forty eight model canines were randomly divided into 4 groups: group A, traditional paraesophagastrie devascularization;group B, selective paraesophagastric devascularization;group C, paraesophagastric devascularization plus distal esophageal transaction and reanastomosis with sta-pler;group D,control group. Every group had 12 canines which were randomly divided into A1 ,B1 ,C1 ,D1, A2,B2,C2 and D2 groups after the operation. Canines in groups A1 ,B1 ,C1 and D1 were executed at first month after the operation,and those in groups A2, B2, C2 and D2 were executed at the 6th month after the operation. The distal esophagus was segmented when the canines were executed. The upside and downside of the stoma in group C were distinguished and signed as Ca and Cb. Immunohistochemieal methods were used to detect VEGF,CD34 and FVⅧ-Rag,blood vessel-related factors,in lower esophagus. Results The expres-sion levels of VEGF and CD34 MVD,FⅧ-Rag MVD in groups A1 ,B1 and C1 were lower than those in group D1 one month after the operation (P <0.05). All the indices in group B1 were lower than those in groups A1 and Clb,but higher than those in group Cla (P <0.05). The indices in group Cla were lower than those in group C1b (P < 0.05 ). The indices in groups A2 and B2 were higher than those in groups A1 and B1, re-spectively at the 6th month after the operation (P <0.05). They were higer in outer membrane in group C1a than in group C2a (P <0.05). The indices in group C2b were higher than those in group Clb (P<0.05). Conclusion At the first month after operation, the expression of blood vessel-related factors was declined in three different procedures of paraesophagastric devascularization,which indicated that all three different pro-cedures could diminish the vessels in distal esophagus. The effect in group C was most obvious among all groups. At the 6th month after traditional paraesophagastrie devascularization and selective paraesophagastrie devascularization, the expression of blood vessel-related factors in distal esophagus was declined. But there were no significant changes in the expression of blood vessel-related factors in distal esophagus after parae-sophagastric devascularization plus distal esophageal transaction and reanastomosis with stapler. It indicated that the blood vessel regeneration in paraesophagastric devascularization pins distal esophageal transaction and reanastomosis with stapler was inferior to that in other procedures.     

不同断流术式对门静脉高压症犬胃黏膜细胞凋亡的影响

目的 检测对不同断流术式前后门静脉高压症犬胃黏膜细胞凋亡,观察其对门脉高压性胃病(PHG)影响.方法 将肝硬化门静脉高压症模型犬24条,随机分为A组(传统断流组)、B组(选择性断流组)、C组(吻合器断流组)及D组(对照组),每组6条.分别于术中及术后6个月测自由门静脉压(FPP),取胃组织,采用原位末端脱氧核苷标记法(TUNEL)染色测凋亡指数(AI)及Caspase-3蛋白免疫组织化学染色计算各染色强度细胞评分总和(H值).结果 术后6个月内,A、B、C组FPP均明显下降,其中以B组下降最为明显,其次为C组,最后为A组.术后6个月,A组AI值由术前的(14.68±1.28)%增全(20.22±2.20)%(P＜0.05),H值由术前的56.13±11.75增至113.78±19.80(P＜0.05);B组AI值由术前的(13.63±1.19)%降至(9.98±2.29)%(P＜0.05),H值由术前的59.13±10.07降至22.35±4.75(P＜0.05).C、D组术后6个月与术前比较,AI和H值无明显变化(P＞0.05).结论 肝硬化门静脉高压症犬行传统断流术术后胃黏膜细胞凋亡加重,PHG病变程度加剧;选择性断流术后胃黏膜细胞凋亡减轻,PHG病变程度改善;吻合器断流术后胃黏膜细胞凋亡变化无差异,对PHG病变影响不大.     

不同门奇断流术式对门静脉高压症犬食管下段血管再生的影响

Objective To explore the effect on blood vessel regeneration of distal esophagus in ca-nines of portal hypertension with liver cirrhosis after different procedures with paraesophagastrie devascular-ization. Methods Portal hypertension models were produced in canines by subcutaneous injection of 60% CC14 combined with food restriction. Forty eight model canines were randomly divided into 4 groups: group A, traditional paraesophagastrie devascularization;group B, selective paraesophagastric devascularization;group C, paraesophagastric devascularization plus distal esophageal transaction and reanastomosis with sta-pler;group D,control group. Every group had 12 canines which were randomly divided into A1 ,B1 ,C1 ,D1, A2,B2,C2 and D2 groups after the operation. Canines in groups A1 ,B1 ,C1 and D1 were executed at first month after the operation,and those in groups A2, B2, C2 and D2 were executed at the 6th month after the operation. The distal esophagus was segmented when the canines were executed. The upside and downside of the stoma in group C were distinguished and signed as Ca and Cb. Immunohistochemieal methods were used to detect VEGF,CD34 and FVⅧ-Rag,blood vessel-related factors,in lower esophagus. Results The expres-sion levels of VEGF and CD34 MVD,FⅧ-Rag MVD in groups A1 ,B1 and C1 were lower than those in group D1 one month after the operation (P <0.05). All the indices in group B1 were lower than those in groups A1 and Clb,but higher than those in group Cla (P <0.05). The indices in group Cla were lower than those in group C1b (P < 0.05 ). The indices in groups A2 and B2 were higher than those in groups A1 and B1, re-spectively at the 6th month after the operation (P <0.05). They were higer in outer membrane in group C1a than in group C2a (P <0.05). The indices in group C2b were higher than those in group Clb (P<0.05). Conclusion At the first month after operation, the expression of blood vessel-related factors was declined in three different procedures of paraesophagastric devascularization,which indicated that all three different pro-cedures could diminish the vessels in distal esophagus. The effect in group C was most obvious among all groups. At the 6th month after traditional paraesophagastrie devascularization and selective paraesophagastrie devascularization, the expression of blood vessel-related factors in distal esophagus was declined. But there were no significant changes in the expression of blood vessel-related factors in distal esophagus after parae-sophagastric devascularization plus distal esophageal transaction and reanastomosis with stapler. It indicated that the blood vessel regeneration in paraesophagastric devascularization pins distal esophageal transaction and reanastomosis with stapler was inferior to that in other procedures.     

吻合器断流术对肝硬化门静脉血流动力学影响的临床评估

目的：评价吻合器断流术对肝硬化患者门静脉系统血流动力学的影响。方法：2002年7月-2009年7月对38例肝硬化门静脉高压症食管静脉曲张破裂出血（esophageal variceal bleeding,EVB）患者分别行传统断流术（A组）及吻合器断流组（B组）,其中A组12例,B组26例,分别于切脾前、后、断流术后测定自由门静脉压力（free portal venous pressures,FPP）。术后3～6个月上消化道造影或胃镜检查了解食管静脉曲张、门脉高压性胃病情况。术后6个月复查彩色多普勒超声检测门静脉直径（portal vein diameter,PVD）、门静脉流速（portal vein velocity,PVV）、门静脉血流量（portal venous flow,PVF）,随访术后再出血情况。结果：A、B两组切脾后门静脉压力较切脾前下降,A组术后FPP为（2.32±0.19）kPa,较切脾后上升,B组术后FPP进一步下降为（1.80±0.15）kPa,较切脾后下降（P〈0.05）,B组术后FPP与A组比较差异有统计学意义（P〈0.05）;术后6个月复查彩超示：B组术后PVD、PVV、PVF均较A组显著减少（P〈0.05）;B组术后食管静脉曲张、门脉高压性胃病显著改善。术后无1例发生再出血。结论：吻合器断流术降低门静脉压力效果较持久,能显著改善食管静脉曲张,并降低术后再出血率,同时不会加剧门脉高压性胃病,是治疗门静脉高压症EVB的一种较理想的术式。     

桡骨远端骨折不同治疗方法的临床疗效分析

目的:探讨针对各种年龄及原因造成的桡骨远端骨折的不同治疗方法的临床疗效分析。方法:选择2011年8月~2013年8月入住我院的58例桡骨远端骨折的患者为研究对象,依据AO分型分为A、B、C 3种类型,并依据其骨折情况及年龄大小和不同方法随机分为3组,其中闭合复位小夹板石膏外固定法治疗24例,切开复位钢板固定法治疗22例,支架外固定法治疗12例。结果:对58例患者进行长达3~6个月的随访,对24例患者实行保守治疗,其中优21例,良2例,可1例,优良率达96.43%;对22例实行切开复位钢板固定手术治疗,其中优15例,良2例,可5例,优良率达78.38%;对12例实行支架外固定手术治疗,其中优7例,良2例,可3例,优良率达77.14%。闭合复位小夹板石膏外固定法组3~6个月膝关节总体情况与切开复位钢板固定法组、支架外固定法治疗组相比,(x2=8.362,8.465,P=0.039,0.037)差异具有统计学意义。结论:闭合复位小夹板石膏外固定法作为传统的治疗桡骨远端骨折的方法疗效比较明显,但不是绝对和固定不变,要结合患者年龄,骨质疏松情况,从事的工作对腕关节功能要求,以及对手术的经济承受能力,才能够制定最适合患者的方案,根据不同桡骨远端骨折患者的年龄及骨折情况,制定不同的治疗方案,才能取得满意的效果。     

生长抑素治疗阑尾切除术后早期炎性肠梗阻的临床分析

目的：探讨生长抑素治疗阑尾切除术后早期炎性肠梗阻的临床效果。方法：回顾性分析海门市人民医院2013年6月—2019 年2月行阑尾切除术后发生早期炎性肠梗阻72例患者的临床资料。其中采用常规保守治疗34例(对照组),在常规治疗的基础上加用生长抑素38例(观察组),比较两组患者胃肠减压量、腹胀缓解时间、排气排便时间、住院时间、住院费用等指标。结果：观察组胃肠减压量、腹胀缓解时间、肛门排气恢复时间及住院时间分别为(492.24±29.93) mL/d、(38.21±8.34) h、(60.25±8.43) h、(256.21±71.64) h,均明显少于对照组[(856.69±39.86) mL/d、(54.85±8.56) h、(83.54±11.93) h、(330.68±82.69) h](均P<0.05);观察组住院费用为(11718.36±578.64) 元,对照组(11562.75±621.42) 元,两组比较差异无统计学意义(P>0.05)。结论：生长抑素治疗阑尾切除术后早期炎性肠梗阻效果显著,可在腹部手术并发术后早期炎性肠梗阻治疗中推广应用。     

不同门奇断流术式对门静脉高压症犬食管下段血管再生的影响

Objective To explore the effect on blood vessel regeneration of distal esophagus in ca-nines of portal hypertension with liver cirrhosis after different procedures with paraesophagastrie devascular-ization. Methods Portal hypertension models were produced in canines by subcutaneous injection of 60% CC14 combined with food restriction. Forty eight model canines were randomly divided into 4 groups: group A, traditional paraesophagastrie devascularization;group B, selective paraesophagastric devascularization;group C, paraesophagastric devascularization plus distal esophageal transaction and reanastomosis with sta-pler;group D,control group. Every group had 12 canines which were randomly divided into A1 ,B1 ,C1 ,D1, A2,B2,C2 and D2 groups after the operation. Canines in groups A1 ,B1 ,C1 and D1 were executed at first month after the operation,and those in groups A2, B2, C2 and D2 were executed at the 6th month after the operation. The distal esophagus was segmented when the canines were executed. The upside and downside of the stoma in group C were distinguished and signed as Ca and Cb. Immunohistochemieal methods were used to detect VEGF,CD34 and FVⅧ-Rag,blood vessel-related factors,in lower esophagus. Results The expres-sion levels of VEGF and CD34 MVD,FⅧ-Rag MVD in groups A1 ,B1 and C1 were lower than those in group D1 one month after the operation (P <0.05). All the indices in group B1 were lower than those in groups A1 and Clb,but higher than those in group Cla (P <0.05). The indices in group Cla were lower than those in group C1b (P < 0.05 ). The indices in groups A2 and B2 were higher than those in groups A1 and B1, re-spectively at the 6th month after the operation (P <0.05). They were higer in outer membrane in group C1a than in group C2a (P <0.05). The indices in group C2b were higher than those in group Clb (P<0.05). Conclusion At the first month after operation, the expression of blood vessel-related factors was declined in three different procedures of paraesophagastric devascularization,which indicated that all three different pro-cedures could diminish the vessels in distal esophagus. The effect in group C was most obvious among all groups. At the 6th month after traditional paraesophagastrie devascularization and selective paraesophagastrie devascularization, the expression of blood vessel-related factors in distal esophagus was declined. But there were no significant changes in the expression of blood vessel-related factors in distal esophagus after parae-sophagastric devascularization plus distal esophageal transaction and reanastomosis with stapler. It indicated that the blood vessel regeneration in paraesophagastric devascularization pins distal esophageal transaction and reanastomosis with stapler was inferior to that in other procedures.     

不同术式疝修补术后发生血清肿的临床分析

目的：比较腹腔镜腹股沟疝修补术与开放式腹股沟疝无张力修补术的术后血清肿发生率,探讨临床合理选择手术方式。方法：腹股沟疝患者211例,其中行开放式腹股沟疝无张力修补术76例,经腹腔镜腹膜前腹股沟疝修补术（TAPP）72例,完全腹膜外腹腔镜腹股沟疝修补术（TEP）63例,比较3种术式术后血清肿的发生情况。结果：开放式腹股沟疝无张力修补术组发生血清肿5例（6.58%）,TAPP组发生血清肿13例（18.06%）,TEP组发生血清肿12例（19.05%）,开放式腹股沟疝无张力修补术组血清肿发生率低于TAPP组和TEP组,差异均有统计学意义（P<0.05）,TAPP组和TEP组血清肿发生率比较,差异无统计学意义（P>0.05）。结论：为降低腹股沟疝修补术术后血清肿的发生,腹股沟疝修补术手术方式的选择应根据患者的具体情况合理选择,对存在发生血清肿高危因素的患者可选择开放式腹股沟疝无张力修补术。     

奥沙利铂联合化疗治疗大肠癌不良反应的观察和护理

目的:总结奥沙利铂联合化疗治疗大肠癌不良反应的观察和护理要点。方法:回顾性分析76例大肠癌患者采用奥沙利铂与5-FU和甲酰四氢叶酸钙(LV)联合化疗出现的不良反应。结果:不良反应主要表现在以下几个方面:发生恶心、呕吐、食欲下降的有48例(63.2%),口腔黏膜炎21例(27.6%),腹泻7例(9.2%),周围神经感觉病变37例(48.7%),骨髓抑制31例(40.7%),发热5例(6.6%)。结论:奥沙利铂作为大肠癌化疗的常用药物,在用药期间应密切观察患者的不良反应,及时采取相应的护理措施,使患者顺利完成化疗。     

不同门奇断流术式对门静脉高压症犬食管下段血管再生的影响

Objective To explore the effect on blood vessel regeneration of distal esophagus in ca-nines of portal hypertension with liver cirrhosis after different procedures with paraesophagastrie devascular-ization. Methods Portal hypertension models were produced in canines by subcutaneous injection of 60% CC14 combined with food restriction. Forty eight model canines were randomly divided into 4 groups: group A, traditional paraesophagastrie devascularization;group B, selective paraesophagastric devascularization;group C, paraesophagastric devascularization plus distal esophageal transaction and reanastomosis with sta-pler;group D,control group. Every group had 12 canines which were randomly divided into A1 ,B1 ,C1 ,D1, A2,B2,C2 and D2 groups after the operation. Canines in groups A1 ,B1 ,C1 and D1 were executed at first month after the operation,and those in groups A2, B2, C2 and D2 were executed at the 6th month after the operation. The distal esophagus was segmented when the canines were executed. The upside and downside of the stoma in group C were distinguished and signed as Ca and Cb. Immunohistochemieal methods were used to detect VEGF,CD34 and FVⅧ-Rag,blood vessel-related factors,in lower esophagus. Results The expres-sion levels of VEGF and CD34 MVD,FⅧ-Rag MVD in groups A1 ,B1 and C1 were lower than those in group D1 one month after the operation (P <0.05). All the indices in group B1 were lower than those in groups A1 and Clb,but higher than those in group Cla (P <0.05). The indices in group Cla were lower than those in group C1b (P < 0.05 ). The indices in groups A2 and B2 were higher than those in groups A1 and B1, re-spectively at the 6th month after the operation (P <0.05). They were higer in outer membrane in group C1a than in group C2a (P <0.05). The indices in group C2b were higher than those in group Clb (P<0.05). Conclusion At the first month after operation, the expression of blood vessel-related factors was declined in three different procedures of paraesophagastric devascularization,which indicated that all three different pro-cedures could diminish the vessels in distal esophagus. The effect in group C was most obvious among all groups. At the 6th month after traditional paraesophagastrie devascularization and selective paraesophagastrie devascularization, the expression of blood vessel-related factors in distal esophagus was declined. But there were no significant changes in the expression of blood vessel-related factors in distal esophagus after parae-sophagastric devascularization plus distal esophageal transaction and reanastomosis with stapler. It indicated that the blood vessel regeneration in paraesophagastric devascularization pins distal esophageal transaction and reanastomosis with stapler was inferior to that in other procedures.