排序方式: 共有15条查询结果,搜索用时 15 毫秒
1.
目的 探讨人类免疫缺陷病毒1型( HIV-1)病毒蛋白r(Vpr)对人结肠癌细胞HCT-8的抑制作用及其机制.方法 将细胞分为空白对照组、空载体转染组和Vpr转染组.空白对照组:细胞不予干预;空载体转染组:对数生长期的细胞加入不同感染复数(MOI)的空载体腺病毒;Vpr转染组:加入不同MOI的含有HIV1-Vpr基因的重组腺病毒(Adv-Vpr).应用MTT比色法检测细胞增殖活性,流式细胞仪检测细胞周期、细胞凋亡及线粒体膜电位,Western blot法检测细胞凋亡相关蛋白的表达.多组间均数比较采用单因素方差分析,两两比较采用q检验;或采用双因素方差分析,组内比较采用t检验.结果 Vpr显著抑制人结肠癌细胞HCT-8增殖,Adv-Vpr转染72 h后(MOI=200),Vpr转染组细胞MTT值为1.03±0.04,与空载体转染组(2.46 +0.15)及空白对照组(2.51±0.14)比较,差异有统计学意义(F=144.6,P<0.05);Adv-Vpr转染48 h后(MOI=200),Vpr转染组处于G2/M期的细胞比例及线粒体膜电位下降的细胞比例增加,分别为37.31%±5.90%和32.07%±5.64%,与空载体转染组(18.30%±6.04%、3.32%±0.79%)及空白对照组916.66%±3.51%、2.76%±1.43%)比较,差异有统计学意义(F=10.08,64.45,P<0.05).Adv-Vpr转染72 h后(MOI=200),Vpr转染组细胞凋亡率为37.62%±6.48%,与空载体转染组(3.44%±1.11%)及空白对照组(2.93%±1.07%)比较,差异有统计学意义(F=122.4,P<0.05).Adv-Vpr处理48 h后(MOI=200),Westem blot检测发现Vpr导致了Caspase-9、Caspase-3剪切为活性片段,p-Chk1-S345磷酸化增加,而Fas、Fas-L、ERK1及ERK2表达未见上调.结论 Vpr在体外能够有效抑制结肠癌细胞株HCT-8增殖,并诱导其细胞周期G2期阻滞及凋亡,其机制分别与DNA损伤信号通路激活及线粒体凋亡通路启动有关.Vpr在结肠癌的治疗中具有潜在的应用前景. 相似文献
2.
3.
目的: 国际上传统的小鼠尿动力学检测方法为膀胱造瘘法。该传统方法破坏膀胱连续性,与临床上广泛采用的经尿道法有明显的差异。本研究寻找到合适的小鼠导尿管材,拟探讨模拟人体经尿道插管法行小鼠尿动力学检测的优势和应用价值。方法: 雌性小鼠8只,选择小儿留置针软芯进行导尿,并作为测压管和膀胱灌注管,经三通外接微量泵以及压力感受器。直肠测压采用硬膜外麻醉导管,外套乳胶手套小指末端一截制成球囊,然后导入小鼠直肠。结果: 该方法成功收集到以下尿动力学指标:膀胱基础压力(BBP)、膀胱漏尿点压(BLPP)、最大排尿压(MVP)、膀胱最大容量(MBC)、残余尿量(PVR)、排尿量(VV)、排尿效率(EV)和膀胱顺应性(BC)。结论: 本研究首次成功模拟人体经尿道插管法,实现小鼠尿动力学检测,成功避免膀胱造瘘对小鼠尿动力学的影响,检测后的小鼠能够长期生存,可以顺利进行后续分子和基因实验。 相似文献
4.
目的:探讨Toll样受体7(TLR7)激动剂刺激肾癌患者外周血单个核细(PBMCs)胞后其抗肿瘤活性的变化。方法:将经TLR7激动剂刺激的肾癌患者外周血单个核细胞与来源于该患者术后标本的原代肾癌细胞共培养。ELISA检测培养基中细胞因子γ-干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)和白细胞介素2(IL-2)水平,流式细胞术检测肾癌细胞周期,[~(51)Cr]释放实验检测PBMCs抗肿瘤活性变化,Western blot检测肾癌细胞Skp2及其下游通路的蛋白水平。结果:TLR7激动剂刺激外周血单个核细胞后,共培养的培养基中IFN-γ、TNF-α和IL-2表达增加,肾癌细胞的增殖指数明显受抑制、PBMCs对肾癌细胞的杀伤率显著提高(P0.05)。肾癌细胞Skp2蛋白水平在刺激后有明显的下降,下降的规律和细胞增殖指数的变化一致;下游p27蛋白水平明显增加,与Skp2蛋白水平变化的规律相反,而p21和p53无明显变化。结论:TLR7激动剂能够有效增强肾癌患者外周血单个核细胞抗肿瘤活性,使肾癌细胞生长受抑制,其机制可能与抑制Skp2/p27通路使细胞周期停滞有关。 相似文献
5.
6.
7.
8.
无张力阴道悬吊系统TVT-S治疗女性压力性尿失禁临床分析 总被引:1,自引:0,他引:1
目的 探讨第三代无张力阴道悬吊系统TVT-S治疗女性压力性尿失禁的适应证和疗效.方法 回顾性分析采用TVT-S术式治疗女性压力性尿失禁27例患者的资料.年龄25~69岁,平均51岁;病程1~20年,平均7年,既往曾行阴道前壁折叠术1例.根据美国妇产科学会POP-Q分级系统评价,Aa为-2~-1 cm,棉签试验尿道夹角>60°,临床症状评估均为Ⅱ度.27例术前尿动力学检查膀胱顺应性正常,最大尿道闭合压(mUCP)25~60 cm H2O(1 cm H2O=0.098 kPa),平均40 cm H2O,腹压漏尿点压(VLPP)均>60 cm H2O.混合性尿失禁1例,单纯型压力性尿失禁26例.观察指标包括手术时间、术后并发症,随访患者的疗效及生活质量.结果 局麻下手术,行U形手术4例(MUCP<30 cm H2O),H形手术23例.手术时间6~15 min,平均8 min.术中失血量少,无尿道及膀胱损伤.术后留置尿管时间平均1 d.术后随访3~15个月,平均8.7个月,26例无需尿垫达到临床治愈,1例混合性尿失禁患者急迫性尿失禁症状改善.I-QOL问卷调查患者生活质量评价良好.25例(93%)患者对术后尿失禁症状改善表示满意,且术前尿失禁症状较重者满意度更高.结论 TVT-S是一种治疗女性压力性尿失禁安全有效且并发症低的微创术式,适合于膀胱顺应性正常、最大尿道闭合压正常、尿道移动度较大的患者,部分高龄或尿道压力较低者建议行U形TVT-S或TVT术.Abstract: Objective To evaluate the effectiveness and indications of a new minimally invasive surgical procedure,the tension-free vaginal tape-SECUR (TVT-S),in the treatment of female stress urinary incontinence (SUI). Methods Twenty-seven consecutive women with stress urinary incontinence underwent the procedure under local anesthesia.The mean age of the study group was 51 (range 25-69)years,average disease duration was 7 (1-20) years.In one case,repair of pelvic floor defects had been made previously.According to the pelvic organ prolapse quantitive(POP-Q) (American College of Obstetrics and Gynecology),the Aa>-1 cm.Swab tests have shown that the angle of urethra Was greater than 60 degrees,while the evaluation of clinical symptoms were grade Ⅱ.The urodynamie test preoperatively of 27 cases display that bladder compliance was normal,mean maximum urethral closure pressure was 40cm H2O(25-60 cm H2O),and VLPP was greater than 60 cm H2O.Minimum follow up was three months (range 3-15 months).The pre-operative maximal urethral cloSHre pressure (MUCP) of all patients was above 30 cm H2O.Ninety-six percent of patients had pure USI,one patient had mixed urodynamic incontinence (MUI).Collection of the data included operative time a well as pre-and post-operative complications.Patients were post-operatively assessed by a validated urinary incontinence-specific measure of Quality of Life (QoL) questionnaire which was completed by all patients at least three months after surgery. Results All patients who underwent the procedure were under local anesthesia.Of these patients,4 cases (MUCP<30 cm H2O) underwent TVT-S with "U" position while the other 23 underwent TVT-S with "Hammock" position.The mean operation time was 8 minutes (range 6-15 min) and blood loss during operation was about 10 ml.There were no urethral and bladder injuries during the procedure.Postoperative indwelling duration was 0-1 day.After follow-up,26 patients were cured without pad usage and 1 with mixed UI had prominent improvement in urgency incontinence symptoms.The quality of life was also improved significantly.93 % patients were satisfied with the postoperative improvement in urinary incontinence symptoms,and the more severe preoperative symptoms the higher satisfaction rate postoperatively. Conclusions TVT-S iS a safe,effective and minimally invasive procedure for SUI with less complication.The patients with normal bladder compliance and MUCP,urethral hypermobility are indicated for this surgery,some elderly patients or patients with low urethral pressure were proposed to undergo "U" position TVT-S or TVT. 相似文献
9.
建立和优化黄芩组织培养技术,为工厂化生产提供有效的技术手段。通过一系列筛选实验,确定建立黄芩无菌材料的最佳方法,黄芩快速繁殖的最佳培养基。结果表明,建立黄芩无菌材料的最佳方法是取试验田里的黄芩主芽或侧芽,用升汞杀菌5 min,无菌水冲洗4~5次,接种在MS+NAA 0.4(mg/L)+IAA 0.2(mg/L)的培养基上,成为无菌试管苗。黄芩试管苗在MS+6-BA 0.3(mg/L)+PP3330.5(mg/L)快繁培养基上,芽多,叶绿并且较壮。在1/2MS+NAA 0.2(mg/L)+IAA(0.1 mg/L)生根培养基中,生根率高,达到90%。 相似文献
10.
目的探讨腹腔镜完全腹膜外疝修补术(laparoscopic totally extraperitoneal,TEP)治疗成人腹股沟疝患者临床疗效、胃肠动力和应激水平变化。
方法回顾性分析2012年1月至2017年6月,徐州市中医院普外科收治的成人腹股沟疝患者60例,随机数字表法分为试验组和对照组,每组患者30例,试验组给予TEP治疗,对照组给予开放式改良Kugel手术治疗,观察2组患者治疗后疗效、疼痛程度、并发症发生率、胃肠动力和应激水平变化。
结果试验组治疗后有效率(93.33%)高于对照组(63.33%),VAS疼痛评分(3.21±0.96)和并发症发生率(16.67%)低于对照组(5.68±1.17,46.67%),血清GAS、MTL、InS和GH水平均明显高于对照组,而血清β-EP、BS水平均明显低于对照组,差异均有统计学意义(P均<0.05)。
结论TEP治疗成人腹股沟疝患者具有良好的临床疗效,可有效改善患者胃肠动力和应激水平。 相似文献