百里醌对人骨肉瘤SaOS-2细胞生长的抑制作用

目的 探讨百里醌对体外人骨肉瘤SaOS-2细胞生长的影响及其机制.方法 百里醌作用人骨肉瘤SaOS-2细胞24h后,细胞计数CCK-8法检测细胞增殖;流式细胞仪检测细胞凋亡;DAPI染色后荧光显微镜下观察细胞形态学变化;Western blot检测SaOS-2细胞中Caspase-3、X连锁凋亡抑制蛋白(XIAP)和Smac的表达.结果 不同浓度(20、40、80μmol/L)百里醌作用人骨肉瘤SaOS-2细胞24h后,细胞存活率分别为(75.5±4.2)%、(62.1±6.7)%和(52.5±4.9)%;细胞早期凋亡率分别为(8.1±0.7)%、(13.2±1.1)%和(20.2±1.7)%;百里醌作用后,SaOS-2细胞出现典型凋亡的形态学改变;百里醌可明显上调Caspase-3和Smac在SaOS-2细胞中的表达,而显著抑制XIAP的表达.结论 百里醌具有抑制体外人骨肉瘤SaOS-2细胞生长和促进细胞凋亡的作用,可能是通过上调人骨肉瘤SaOS-2细胞中Caspase-3和Smac的表达和下调XIAP的表达而实现.

Abstract：

Objective To investigate the inhibitory effect of thymoquinone on the growth of human osteosarcoma cell line SaOS-2 in vitro.Methods After human osteosarcoma SaOS-2 cells were treated with different concentrations of thymoquinone, the proliferation was measured by Cell Counting Kit-8 (CCK-8) assay. The flowcytometry (FCM) was used to determine apoptosis of SaOS-2 cells. The morphological changes of SaOS-2 cells were observed under the fluorescence microscopy after DAPI staining. Western blotting was used to detect the protein expression of Caspase-3, X-linked inhibitor of apoptosis protein (XIAP) and Smac.Results Thymoquinone obviously suppressed proliferation of SaOS-2 cells in a dose-dependent manner, and the apoptosis rate was (8.1±0.7)%, (13.2±1.1)% and (20.2±1.7)% respectively when the concentrations of thymoquinone exposed were 20, 40 and 80 μmol/L. After treatment with thymoquinone, the expression of Caspase-3 and Smac was up-regulated in SaOS-2 cells, and thymoquinone treatment significantly decreased the XIAP levels in SaOS-2 cells.Conclusion Thymoquinone has the anti-tumor effect on the human osteosarcoma SaOS-2 cells in vitroprobably by up-regulating the expression of Caspase-3 and Smac and down-regulating the expression of XIAP.     

医护人员突发公共卫生事件知识调查分析

目的：考察突发公共卫生事件下,医护人员对事件相关知识和信息的了解状况,及其对情绪的影响。方法：采用自编调查问卷,调查一所综合性医院中各类医护人员。结果：突发公共卫生事件下,各类医护人员对与突发事件相关的知识和信息的了解以及信息来源,均无显著差异。较之本院工作人员,外院进修人员对信息透明和知情的要求最低。在知情的情况下治疗和护理SARS病人,医护人员更多体验正性情绪;而不知情的情况下的情绪反应更多是负性的。结论：在突发公共卫生事件下,应让医护人员及时充分地享有和运用各类信启、资源,增强心理储备和情绪调节,避免因信息缺失带来过多负性情绪反应。     

等离子刀与机械刨削在治疗膝骨性关节炎性滑膜炎中的作用分析

目的：比较等离子刀与机械刨削在治疗膝骨性关节炎性（OA）滑膜炎中的作用。方法：采用随机对照前瞻性研究,等离子刀治疗组90例,机械刨削对照组96例,全部病例获得6～36个月(平均19.4个月)随访。术后分别比较两组患者的Lysholm评分,术后康复时间及关节积液发生情况。结果：等离子刀组康复时间较常规器械组明显缩短,关节积液发生率明显减少,术后2周Lysholm评分显著优于同期的常规器械组(P＜0.05）。结论：等离子刀对于OA滑膜炎的病例是首选治疗方法。