具有β-半乳糖苷酶转苷能力的菌株筛选及鉴定

以乳糖为惟一碳源,5-溴-4-氯-3-吲哚- β -D-半乳糖苷(X-gal)为显色剂,从土壤中筛选出10株产β- 半乳糖苷酶较高、生长较好的菌株.在30 ℃下发酵产酶,测定邻硝基苯- β- D-半乳糖苷(ONPG)水解能力.在50 mmol/L磷酸钾缓冲液(pH 7.0)中,加入400 g/L乳糖和200 g/L果糖,并分别添加10株菌所产β- 半乳糖苷酶, 至酶活为400 U/L,37 ℃下反应8 h,经高效液相色谱分析,编号为2-1样品中含有乳果糖.通过形态特征和16S rDNA 序列分析,鉴定菌株2-1为节杆菌属.     

产蓝色素放线菌的初步鉴定和蓝色素性质研究

对一株从土壤中筛选出来的产蓝色素的放线菌进行了初步鉴定, 命名为天蓝色链霉菌－１００（Ｓｔｒｅｐｔｏｍ ｙｃｅｓ ｃｏｅｌｉｃｏｌｏｒ－１００ ）,并对蓝色素的性质进行了研究．结果表明此色素无臭无味,水溶性较强．酸性条件下,溶解度随ｐＨ 降低而减小．ｐＨ＜ ８ 时为红色,ｐＨ≥８ 时为蓝色,颜色随ｐＨ 增大而加深．于１００ ℃短时间处理,比较稳定．在ｐＨ 中性的条件下对光（包括紫外光）较稳定．大多数金属离子如Ｂａ２＋ 、Ｋ＋ 等对此色素无影响,Ｍｇ２＋ 使色素增色．Ｆｅ２＋ 在５×１０－４ ｍ ｏｌ／Ｌ时,使色素变性,但在５×１０－５ ｍ ｏｌ／Ｌ时,对色素没有多大影响．Ｐｂ２＋ 可以络合色素,使之沉淀;加入０．２５％ 盐酸乙醇后,色素再溶解．该蓝色素可作为天然色素或天然ｐＨ 指示剂开发．     

神经导航及内镜辅助下经鼻蝶显微切除无功能性 垂体大腺瘤的近期疗效分析

ObjectiveTo investigate the short term effect of neuronavigator and endoscope assisted endonasal trans sphenoidal surgery for patients with nonfunctioning pituitary macroadenomas.MethodsClinical data of 62 patients with nonfunctioning pituitary macroadenomas received surgical treatment in our department from Jan 2013 to Sept 2014 were retrospectively analyzed. 62 patients were divided into two groups by whether to use neuronavigator and endoscopic technology or not, i.e. study group (32 cases) and control group (30 cases). Pituitary adenoma resection was performed with assistance of endoscope and neuronavigation in the study group. The indexes including surgery time, postoperative hospitalization duration, postoperative complications, changes of serum endocrinology, MRI, the residual and recurrence rates in the two groups were recorded and analyzed.ResultsThe time of hospital stays (6.6±0.7 d) in the study group was significantly less than that in control group (8.1±0.8 d). The postoperative complication rate (including transient diabetes insipidus, cerebrospinal fluid rhinorrhea, electrolyte disturbances, postoperative bleeding in the tumor cavity,hypopituitarism) in study group was also significantly lower than that in the control group (P<0.05). The postoperative remission rate of study group was significantly higher than that of the control group. The residual (2 cases) and recurrence (0 cases) rates in the study group were significantly lower than those in the control group (9 cases and 5 cases). According to the Knosp grade, the residual rate of the patients with Knosp grade=2 in the study group was lower than that of the control group, and the difference was statistically significant (P<0.05).ConclusionThe neuronavigator and endoscopic technology contribute to decrease the occurrence of the postoperative complications, to reduce residual and recurrence rate, and to improve the gross total resection of tumor, is therefore effective for the treatment for patients with nonfunctioning pituitary macroadenomas.     

成人颅内高级别脑膜瘤的长期随访研究

目的 评估不同治疗策略下高级别脑膜瘤患者的临床预后情况,探讨未来前瞻性研究的相关指标.方法 回顾性分析206例高级别脑膜瘤患者的流行病学、诊断、治疗、复发信息和生存数据.结果 本组患者总体而言,中位无进展生存期(PFS)为3.9年,中位总生存期(OS)为9.7年.手术切除范围不仅对提高患者的生存率具有重要意义(全切除v...     

叶绿酸酯的光敏杀菌作用

广泛使用抗生素治疗细菌感染是导致抗生素抗性菌增加的重要原因,研究杀菌的其他方法,如光敏杀菌法等十分必要.研究结果表明,叶绿素的降解产物叶绿酸酯对白葡萄球菌(Staphy lococcusalbus)有显著的光动力杀伤作用,而对大肠杆菌(Escherichiacoli)的生长没有影响;葡萄球菌较大肠杆菌能更有效地吸附叶绿酸酯,导致对生长的抑制.     

脱镁叶绿酸a对金黄色葡萄球菌、大肠杆菌的抑制作用

以叶绿素为提取物，进行化学修饰得到光敏活性剂-脱镁叶绿酸α，其对微生物生长影响的研究表明：在光照条件下，脱镁叶绿酸α对金黄色葡萄球菌的生长和大肠杆菌原生质体的再生有明显的抑制作用；在暗培养条件下，脱镁叶绿酸α的抑制作用减弱。     

成人幕上脑胶质瘤切除术中进入脑室相关并发症的回顾性分析

目的 探讨脑胶质瘤切除术中进入脑室是否会引发更多的并发症及其对预后的影响。方法 回顾性分析2014年1月至2016年12月该院开颅行幕上脑胶质瘤切除术的975例患者资料,且对手术过程中进入脑室系统、术后使用脑室外引流、脑室内出血、感染和脑积水等因素进行回顾性分析。结果 进入脑室和未进入脑室患者的性别、肿瘤类型、世界卫生组织(WHO)分级、肿瘤总体积、到脑室的距离、术中出血量比较,差异有统计学意义(P<0.05)。进入脑室患者的脑室外引流、脑室内出血、帽状腱膜下积液、切口感染、脑积水、脑室腹腔分流管置入的发生率更高,差异有统计学意义(P<0.05)。未进入脑室患者的中位生存期较进入脑室患者更长(526 d vs. 309 d),差异有统计学意义(HR=1.22,P=0.001 2)。结论 成人幕上脑胶质瘤切除手术中未进入脑室的并发症发生情况更少。