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1.
2.
R Kawaguchi Y Shibuya U Ogasa O Kosuda K Hikiji K Ishii 《Rinsho byori. The Japanese journal of clinical pathology》1992,40(11):1198-1203
Six kinds of human herpes viruses have been identified and classified on the basis of structure and characteristics. We studied the identification and classification of these types using PCR to amplify the virus-specific DNA sequences. This method showed higher sensitivity than the conventional method of virus isolation and culture for HSV and CMV detection. For each positive control, the viral DNA was amplified only when the complementary primers themselves were used. PCR apparently detects only the activated virus, because normal controls were negative when this method was used. Therefore, the present method is thought to closely reflect viral activation and infectious diseases in patients with latent infections. 相似文献
3.
Noriko Kimura Mika Watanabe Tsuneo Ookuma Wakako Miura Takao Noshiro Yukio Miura Hiroshi Nagura 《Endocrine pathology》1994,5(3):178-182
Pheochromocytoma usually shows prominent nuclear atypia, but the presence of such atypical cells is known to be an unreliable
predictor of malignancy. DNA ploidy of pheochromocytomas has been analyzed by flow cytometry or photospectrometry on paraffinem-bedded
tissue, but the results were controversial. We performed DNA analysis on cytology specimens of 11 pheochromocytomas using
an image analysis system. All tumors had a mixed pattern of a large population of diploid cells and a small population of
polyploid cells. DNA content correlated with nuclear size, and larger cells had more DNA content. Such larger tumor cells
had polyploid nuclei, such as 4 C, 8 C, 16 C, and 32 C, in both malignant and benign pheochromocytomas. The larger polyploid
nuclei may result from difficulty of duplication at the mitotic phase of the cell cycle. 相似文献
4.
Summary The host-vector system of an n-lkaneassimilating-yeast, Candida maltosa, which we previously constructed using an autonomously replicating sequence (ARS) region isolated from the genome of this yeast, utilizes C. maltosa J288 (leu2
–) as a host. As this host had a serious growth defect on n-alkane, we developed an improved host-vector system using C. maltosa CHI (his) as host. The vectors were constructed with the Candida ARS region and a DNA fragment isolated from the genome of C. maltosa. Since this DNA fragment could complement histidine auxotrophy of both C. maltosa CH1 and S. cerevisiae (hiss
–), we termed the gene contained in this DNA fragment C-HIS5. The vectors were characterized in terms of transformation frequency and stability, and the nucleotide sequence of C-HISS was determined. The deduced amino acid sequence (389 residues) shared 51% homology with that of HISS of S. cerevisiae (384 residues; Nishiwaki et al. 1987). 相似文献
5.
Kouji Katsura Yoshihiko Soga Sadatomo Zenda Hiromi Nishi Marie Soga Masatoshi Usubuchi Sachiyo Mitsunaga Ken Tomizuka Tetsuhito Konishi Wakako Yatsuoka Takao Ueno Tadanobu Aragaki Takafumi Hayashi 《Journal of radiation research》2021,62(2):374
The aim of this study was to compare the estimated public medical care cost of measures to address metallic dental restorations (MDRs) for head and neck radiotherapy using high-energy mega-voltage X-rays. This was considered a first step to clarify which MDR measure was more cost-effective. We estimated the medical care cost of radiotherapy for two representative MDR measures: (i) with MDR removal or (ii) without MDR removal (non-MDR removal) using magnetic resonance imaging and a spacer. A total of 5520 patients received head and neck radiation therapy in 2018. The mean number of MDRs per person was 4.1 dental crowns and 1.3 dental bridges. The mean cost per person was estimated to be 121 720 yen for MDR removal and 54 940 yen for non-MDR removal. Therefore, the difference in total public medical care cost between MDR removal and non-MDR removal was estimated to be 303 268 800 yen. Our results suggested that non-MDR removal would be more cost-effective than MDR removal for head and neck radiotherapy. In the future, a national survey and cost-effectiveness analysis via a multicenter study are necessary; these investigations should include various outcomes such as the rate of local control, status of oral mucositis, frequency of hospital visits and efforts of the medical professionals. 相似文献
6.
Yoko Okumura MSc Yoshihisa Nasa PhD Atsushi Sanbe PhD Wakako Toga BSc Satoshi Takeo PhD 《Journal of cardiac failure》1996,2(4):301-310
Background:
Despite the clinical relevance of angiotensin I-converting enzyme (ACE)inhibitors, their effects on impaired vascular function in patients and animals with chronic heart failure (CHF) have not been fully understood. This study was undertaken to determine whether long-term treatment with an ACE inhibitor improved the altered contractile properties of vessels from rats with CHF.Methods and Results:
Twelve weeks after coronary artery ligation, the rats were sacrificed and the isometric tension development of thoracic aorta, pulmonary artery, and mesenteric artery with and without endothelium was examined. Contractile responses to norepinephrine and prostaglandin F2α were augmented in endothelium-intact, but not in endothelium-denuded, thoracic aorta and pulmonary artery segments of the rat with CHF. The contractile response to angiotensin II was augmented in endothelium-denuded mesenteric artery segments of the rat with CHF, which was attenuated by indomethacin or diclofenac sodium but not by bunazosin. Trandolapril (3 mg/kg/d) was administered orally from the 2nd to 12th week after the operation. Treatment with trandolapril reversed the augmented contractile response of the rat with CHF to norepinephrine, prostaglandin F2α, and angiotensin II almost to the levels in the sham-operated rat.Conclusions:
The results demonstrate that an ACE inhibitor is capable of reversing altered vascular function in the rat with CHF, suggesting that vascular beds are possible sites of action for ACE inhibitors in the therapy for CHF. 相似文献7.
Yasuhiro Hamada Kazushi Deguchi Kisaki Tachi Makoto Kita Wakako Nonaka Tadayuki Takata Hideki Kobara Tetsuo Touge Katsuya Satoh Tsutomu Masaki 《Internal medicine (Tokyo, Japan)》2022,61(17):2667
A 63-year-old woman who presented for orofacial dystonia showed cortical ribboning, a typical MRI finding in sporadic Creutzfeldt-Jakob disease (sCJD). However, real-time quaking-induced conversion (RT-QuIC), the most sensitive method for an early diagnosis of sCJD, was negative. She developed sCJD six months later, at which time RT-QuIC became positive. The cerebral blood flow showed a decrease in the cerebral cortex (especially in the supramarginal gyrus) consistent with cortical ribboning, but an increase in the basal ganglia, probably involved in orofacial dystonia. Cortical ribboning on MRI might be a better biomarker than RT-QuIC in the prodromal phase of sCJD. 相似文献
8.
Orexin-A is a newly identified neuropeptide expressed in the lateral areas of the hypothalamus that plays a role in various physiological functions, including regulation of glucose metabolism. We have previously reported that the development of post-ischemic glucose intolerance is one of the triggers of ischemic neuronal damage. Therefore, the aim of this study was to determine the effects of orexin-A on the development of post-ischemic glucose intolerance and ischemic neuronal damage. Male ddY mice were subjected to middle cerebral artery occlusion (MCAO) for 2 h. Neuronal damage was estimated by histological and behavioral analysis after MCAO. Intracerebroventricular administration of orexin-A (2.5, 25, or 250 pmol/mouse) significantly and dose-dependently suppressed the development of post-ischemic glucose intolerance on day 1 after MCAO and neuronal damage on day 3 after MCAO. In the liver and skeletal muscle, the expression levels of insulin receptor were decreased, whereas those of gluconeogenic enzymes were increased on day 1 after MCAO. Furthermore, these expressions were completely recovered to normal levels by orexin-A and were reversed by the administration of SB334867, a specific orexin-1 receptor antagonist. These results suggest that regulation of post-ischemic glucose intolerance by orexin-A suppressed cerebral ischemic neuronal damage. 相似文献
9.
Hideki Ishikura Takeya Ono Sadaaki Oki Yasukazu Saito Namiko Umei Wakako Tsumiyama Atsushi Tasaka Kazuki Aihara Yuta Sato Tomohiro Matsumoto Akira Otsuka 《Journal of Physical Therapy Science》2015,27(9):2821-2823
[Purpose] The purpose of this study was to investigate how a stretching torque affects
muscular contractures. [Subjects] The subjects of this study were 48 male Wistar rats.
[Methods] Subjects were divided into 4 groups as follows: Group 1 was the control; Group 2
had muscles in continuous fixation; Group 3 had muscles stretched in the direction of
dorsiflexion by a spring balancer set at a torque of 0.3N for a period of 30 minutes after
continuous fixation; and Group 4 had muscles stretched in the direction of dorsiflexion by
a spring balancer set at a torque of 3.0N for a period of 30 minutes after continuous
fixation. Joint fixation periods were for 2 and 4-weeks. Ankle joint range of motion and
soleus flexibility were analyzed. [Results] For the 2-week joint fixation, soleus
flexibility in Group 4 showed an increase compared with that of Group 3. For both fixation
periods, range of motion in Group 4 showed an increase compared with that of Group 3.
[Conclusion] For both fixation periods, stretching improved joint range of motion. In the
2-week joint fixation, soleus flexibility improved. However, soleus flexibility did not
improve in the 4-week joint fixation.Key words: Muscular contracture, Stretching, Muscular flexibility 相似文献
10.
Hidetaka Tamune Hiroaki Takeya Wakako Suzuki Yasuaki Tagashira Takaie Kuki Mitsuhiro Nakamura 《The American journal of emergency medicine》2013,31(11):1601-1604
BackgroundMeningitis is a common emergency disease. Signs and symptoms easily observed at the bedside are needed because early recognition of the possibility of meningitis is necessary for the decision to perform lumbar puncture. Jolt accentuation of headache has been reported to be the most sensitive diagnostic test; however, limited articles have reproduced its sensitivity.MethodsThis is a single-center retrospective medical record review between 2007 and 2012. We diagnosed meningitis based on the criterion standard that cerebrospinal fluid total cells is more than 5/mm3, in accordance with previous studies. All diagnostic and management decisions including Kernig sign, nuchal rigidity, and jolt accentuation of headache were at the physician's discretion. We calculated the sensitivity and specificity of well-known signs and symptoms of meningitis and, especially, compared the efficacy of jolt accentuation of headache with previous studies.ResultsWe investigated 531 adult patients who were suspected of meningitis and had lumbar puncture performed. Of these patients, 139 had meningitis. Background characteristics and vital signs were not clinically different between the 2 groups, although classic tetralogy of bacterial meningitis (fever, nuchal rigidity, mental disturbance, and headache) was worth investigated. The sensitivity and specificity of jolt accentuation of headache were 63.9% (95% confidence interval, 51.9%-76.0%) and 43.2% (34.7%-51.6%), respectively.ConclusionThe absence of jolt accentuation of headache test cannot, on its own, accurately rule out meningitis in adults. Further studies are warranted to reproduce this result and to discover better bedside diagnostic tests. 相似文献