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J. Stelling H. Dupuis 《International archives of occupational and environmental health》1996,68(4):236-242
Under laboratory conditions the effects of single-axis and multi-axis hand-arm vibration exposure on several strain parameters were tested in up to 20 male subjects. As parameters of these acute effects, the biodynamic vibration behavior of the hand-arm system, the electrical activity of the most affected muscle groups, the skin temperature, the vibration sensitivity of the fingertips, and the subjective vibration sensation were measured. When comparing simulated three-axis vibration exposure with single-axis vibration exposure, synergistic effects in the form of an increasing reaction could be found. It could be proven that the vector sum of the frequency-weighted acceleration in the three axes represents the acute effects better than does the weighted acceleration in the main axis alone. This summation has to take into account the relatively lower effects of vibration in the x- or y-direction compared with the z-direction. On the basis of the experimental results a new proposal for frequency weighting of the three different axes and an energetic summation procedure are derived. Application of this knowledge in the International Standard ISO 5349 is proposed. 相似文献
8.
Sequence Analysis of the RNA Polymerase Gene of Foot-and-Mouth Disease Virus Serotype Asia1 总被引:1,自引:0,他引:1
George M Venkataramanan R Pattnaik B Sanyal A Gurumurthy CB Hemadri D Tosh C 《Virus genes》2001,22(1):21-26
The complete nucleotide (nt.) sequence of the RNA polymerase (3D) gene and 81 nt. in the 3-untranslated region of foot-and-mouth disease virus (FMDV) serotype Asia1 (IND63/72) was determined and compared with the sequence of other FMDV serotypes. The 3D genomic region was 1410 nt. long encoding 470 amino acids with an inframe stop codon (TAA) at nt. position 1411–1413. The deduced amino acid sequence of the protein showed 8 conserved motifs as reported in other picornaviruses, 2 of which are 100% identical across the serotypes. Antigenic regions in the polymerase protein were predicted and found to be located at the N-terminus of the protein. The phylogenetic analysis showed that the FMD viruses were segregated into different clusters based on geographical origin; the Asia1 virus did not cluster tightly with any of the geographical groups. 相似文献
9.
Ability of laboratories to detect emerging antimicrobial resistance: proficiency testing and quality control results from the World Health Organization's external quality assurance system for antimicrobial susceptibility testing 总被引:12,自引:0,他引:12 下载免费PDF全文
Tenover FC Mohammed MJ Stelling J O'Brien T Williams R 《Journal of clinical microbiology》2001,39(1):241-250
The accuracy of antimicrobial susceptibility data submitted by microbiology laboratories to national and international surveillance systems has been debated for a number of years. To assess the accuracy of data submitted to the World Health Organization by users of the WHONET software, the Centers for Disease Control and Prevention distributed six bacterial isolates representing key antimicrobial-resistance phenotypes to approximately 130 laboratories, all but one of which were outside of the United States, for antimicrobial susceptibility testing as part of the World Health Organization's External Quality Assurance System for Antimicrobial Susceptibility Testing. Each laboratory also was asked to submit 10 consecutive quality control values for several key organism-drug combinations. Most laboratories were able to detect methicillin (oxacillin) resistance in Staphylococcus aureus, high-level vancomycin resistance in Enterococcus faecium, and resistance to extended-spectrum cephalosporins in Klebsiella pneumoniae. Many laboratories, particularly those using disk diffusion tests, had difficulty in recognizing reduced susceptibility to penicillin in an isolate of Streptococcus pneumoniae. The most difficult phenotype for laboratories to detect was reduced susceptibility to vancomycin in an isolate of Staphylococcus epidermidis. The proficiency testing challenge also included a request for biochemical identification of a gram-negative bacillus, which most laboratories recognized as Enterobacter cloacae. Although only a small subset of laboratories have submitted their quality control data, it is clear that many of these laboratories generate disk diffusion results for oxacillin when testing S. aureus ATCC 25923 and S. pneumoniae ATCC 49619 that are outside of the acceptable quality control range. The narrow quality control range for vancomycin also proved to be a challenge for many of the laboratories submitting data; approximately 27% of results were out of range. Thus, it is important to establish the proficiency of laboratories submitting data to surveillance systems in which the organisms are tested locally, particularly for penicillin resistance in pneumococci and glycopeptide resistance in staphylococci. 相似文献
10.
Comparison of ultrasonographic findings in spontaneous abortions with normal and abnormal karyotypes 总被引:2,自引:2,他引:2
To determine whether ultrasonographic findings can predict the karyotype of
spontaneous abortions, 137 pregnancies (54 spontaneous, 83 assisted
ovulatory cycles) that subsequently aborted and had chromosome analysis
performed on the products of conception were studied ultrasonographically.
Transvaginal ultrasound was performed using an Acuson 128XP/10 with 7.5 MHz
probe. The numbers of empty gestational sacs, small and normal for
gestational size, embryonic poles and embryos with documented cardiac
activity were calculated. The frequency of each of these findings in
pregnancies with normal and abnormal karyotypes was compared. Of the 137
spontaneous abortions, 51 had normal chromosome analyses and 86 had
abnormal karyotypes (68 aneuploidies and 18 polyploidies). Ultrasonographic
findings in the 51 karyotypically normal pregnancies included 16 (31%) with
empty gestational sacs, and 35 (69%) with embryonic poles, of which 24
(69%) were at least 1 week smaller than expected for gestational age and 11
(31%) were the expected size. Embryonic cardiac activity was documented in
22 (63%) of the 35 embryonic poles. Amongst 86 pregnancies with abnormal
karyotypes, similar frequencies of ultrasound findings were found: 23 (27%)
with empty gestational sacs, 42 (67%) with embryonic poles smaller than
expected for gestational age, and 50 (79%) embryos lost after documentation
of embryonic cardiac activity. No differences in the frequency of
ultrasonographic findings of empty gestational sacs, small embryonic pole
and embryonic cardiac activity were observed between karyotypically normal
and abnormal spontaneous abortions. Ultrasonographic findings cannot
predict the karyotype of spontaneous abortions.
相似文献