全文获取类型
收费全文 | 528篇 |
免费 | 24篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 15篇 |
妇产科学 | 4篇 |
基础医学 | 38篇 |
口腔科学 | 6篇 |
临床医学 | 22篇 |
内科学 | 58篇 |
皮肤病学 | 4篇 |
神经病学 | 39篇 |
特种医学 | 64篇 |
外科学 | 88篇 |
综合类 | 44篇 |
一般理论 | 1篇 |
预防医学 | 9篇 |
眼科学 | 108篇 |
药学 | 25篇 |
1篇 | |
肿瘤学 | 25篇 |
出版年
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 8篇 |
2019年 | 8篇 |
2018年 | 9篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 11篇 |
2014年 | 16篇 |
2013年 | 13篇 |
2012年 | 23篇 |
2011年 | 21篇 |
2010年 | 13篇 |
2009年 | 11篇 |
2008年 | 22篇 |
2007年 | 21篇 |
2006年 | 21篇 |
2005年 | 22篇 |
2004年 | 15篇 |
2003年 | 6篇 |
2002年 | 10篇 |
2001年 | 9篇 |
2000年 | 14篇 |
1999年 | 15篇 |
1998年 | 15篇 |
1997年 | 23篇 |
1996年 | 19篇 |
1995年 | 10篇 |
1994年 | 11篇 |
1993年 | 15篇 |
1992年 | 7篇 |
1991年 | 12篇 |
1990年 | 9篇 |
1989年 | 9篇 |
1988年 | 4篇 |
1987年 | 13篇 |
1986年 | 6篇 |
1985年 | 6篇 |
1983年 | 4篇 |
1978年 | 7篇 |
1977年 | 4篇 |
1976年 | 10篇 |
1974年 | 7篇 |
1973年 | 6篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1969年 | 5篇 |
1967年 | 5篇 |
1966年 | 3篇 |
1954年 | 3篇 |
排序方式: 共有555条查询结果,搜索用时 0 毫秒
1.
2.
3.
4.
Helmut Brunner Werner Schaeg Ursula Brück Ulrich Schummer Hans-Gerd Schiefer 《Medical microbiology and immunology》1977,163(1):25-35
A radioimmunoassay (RIA) which depends on the property of protein A ofStaphylococcus aureus to combine with the Fc-fragment of immunoglobulins was developed.This technique was employed to measure antibodies in human and various animal sera. It could be demonstrated that the staphylococcal RIA was at least as sensitive as the previously described radioimmunoprecipitation technique in detecting antibodies toM.pneumoniae in human sera. In addition, antibodies toM.pneumoniae could be demonstrated in sera of hamsters intranasally inoculated with the organisms. Antibodies could also be demonstrated in rabbit sera after immunization withM.pneumoniae. The test proved to be considerably more sensitive than conventional tests for detection of antibodies to the organisms. The test requires only small amounts of reagents and is relatively inexpensive.The results were presented in a preliminary form at the annual meeting of the Local Branch of the American Society for Microbiology, Frankfurt, March 1976 and the 77th ordinary meeting of the Society for General Microbiology, Glasgow, September 1976 相似文献
5.
Neulen J; Raczek S; Pogorzelski M; Grunwald K; Yeo TK; Dvorak HF; Weich HA; Breckwoldt M 《Molecular human reproduction》1998,4(3):203-206
Vascularization is a prominent event during corpus luteum formation,
providing low density lipoproteins for steroid biosynthesis and enabling
transport of secreted steroids. The process of vascularization is
controlled by specific regulators. Vascular endothelial growth factor
(VEGF), otherwise named vascular permeability factor (VPF), induces
endothelial cell proliferation as well as angiogenesis in vivo and
increases capillary permeability. Here we report the expression of VEGF/VPF
mRNA by cultured human luteinized granulosa cells (GC) for at least 10
days. Without HCG VEGF/VPF expression declined after day 4 and by day 10
was reduced to approximately 30% of the value at day 4. However, after
culture in the presence of 1 U/ml human chorionic gonadotrophin (HCG),
expression of VEGF/VPF mRNA by GC was four times greater than control
experiments by day 10, and increased 100% from day 4 to day 10.
Simultaneously, HCG supplementation increased VEGF/VPF secretion by GC.
Medium VEGF/VPF on day 3 was 13 pM without and 11 pM with HCG. Medium
VEGF/VPF on day 10 was 6 pM without HCG and 29 pM with HCG. These results
suggest that vascularization of the corpus luteum is induced by
HCG-mediated effects of VEGF/VPF.
相似文献
6.
Electron microscopic studies on the attachment of Mycoplasma pneumoniae to guinea pig erythrocytes. 下载免费PDF全文
A mechanism of pathogenicity of Mycoplasma pneumoniae is its ability to attach to the surface of mammalian cells. It has previously been demonstrated by others that M. pneumoniae adheres with a specialized terminal structure, the "tip," to ciliated epithelial cells of the respiratory tract. In this report we show by electron microscopy that M. pneumoniae adsorbs with membrane sites other than the tip to guinea pig erythrocytes. 相似文献
7.
Lesions of the articular surfaces of the knee have been managed by various techniques over the last 50 years. Surgical management has involved: excising the damaged area, refashioning the underlying bone to produce a fibrous response, and introducing allograft, autograft and synthetic materials to encourage a repair matrix. The techniques and their pitfalls are reviewed and discussed, and suggestions made as to the direction of future studies for the repair of osteochondral lesions in the painful knee. 相似文献
8.
This paper describes the Topcon "Ophthalmic Viewer" and especially its application for evaluating photographs of the corneal endothelium. 相似文献
9.
Marc Daniels Paul Christian Fuchs Rolf Lefering Daniel Grigutsch Harun Seyhan Ulrich Limper Jennifer Lynn Schiefer 《Burns : journal of the International Society for Burn Injuries》2021,47(4):914-921
BackgroundR Rapid fluid resuscitation is a crucial therapy during the treatment of patients with extensive burns. In 1968, the Parkland Formula was introduced for the calculation of the estimated volume of the resuscitation fluid. Since then, different methods for the calculation of fluid resuscitation volume have been developed. We aimed to evaluate if the Parkland formula is still the most effective method for fluid resuscitation volume calculation in burn patients.MethodsIn the period between January 2015 and January 2019, data from 569 patients over 16 years old with burns of more than 20% total body surface area (TBSA) and at least 15% TBSA full thickness burns were entered in the German burn registry. The patients were divided into 5 groups (0, +1, ?1, +2, ?2) according to the volume of the resuscitation fluid they received. Group 0 patients received the amount of fluid calculated according to the Parkland formula (n = 83). The 4 other groups received reduced (-1, -2) or increased (+1, +2) fluid volumes in comparison to the value obtained by the Parkland formula.ResultsPatients in Group 0 presented a significantly lower mortality in the first week (4.5%) compared to groups –2 (16.7%) and group +2 (19.5%) (p = 0.021). Furthermore, the mean number of operations in group +2 (5.81) was higher than in group ?2 (3.81). Surviving patients from group +2 presented a longer hospital stay (68.1 days) compared to the other groups. Additionally, the logistic regression analysis showed a higher survival of patients in groups ?2 and ?1 (regression coefficients ?0.11 and ?0.086; Odds Ratio 0.896 and 0.918; 95% Confidence Interval (CI) 0,411–1.951 and 0.42–2.004).ConclusionIn this retrospective study, register based analysis a restrictive fluid regime was associated with a higher survival compared to the liberal Parkland guided fluid regime. 相似文献
10.
Renal transplant (RT) is now a therapy of choice for end stage renal disease (ESRD). The Nephrology Unit, Asvini started functioning in Dec 90 and to date 1298 sittings of hemodialysis have been given to 45 patients. Of these, 35 were in ESRD and 11 patients underwent renal transplantation at this hospital during the period Jan 91 – Dec 93. One patient expired after 18 months of transplantation due to infection. Early experience in screening patients for RT, use of immunosuppression, management of rejection episodes and protocol are presented with special emphasis on its relevance to the Armed Forces.KEY WORDS: Transplantation, Renal Failure, Immunosuppression, Rejection 相似文献