全文获取类型
收费全文 | 1127篇 |
免费 | 69篇 |
专业分类
耳鼻咽喉 | 120篇 |
儿科学 | 10篇 |
妇产科学 | 7篇 |
基础医学 | 151篇 |
口腔科学 | 45篇 |
临床医学 | 87篇 |
内科学 | 223篇 |
皮肤病学 | 32篇 |
神经病学 | 100篇 |
特种医学 | 31篇 |
外科学 | 99篇 |
综合类 | 6篇 |
一般理论 | 1篇 |
预防医学 | 51篇 |
眼科学 | 88篇 |
药学 | 76篇 |
肿瘤学 | 69篇 |
出版年
2023年 | 6篇 |
2022年 | 11篇 |
2021年 | 37篇 |
2020年 | 20篇 |
2019年 | 30篇 |
2018年 | 37篇 |
2017年 | 20篇 |
2016年 | 24篇 |
2015年 | 36篇 |
2014年 | 38篇 |
2013年 | 41篇 |
2012年 | 69篇 |
2011年 | 69篇 |
2010年 | 54篇 |
2009年 | 22篇 |
2008年 | 46篇 |
2007年 | 50篇 |
2006年 | 41篇 |
2005年 | 45篇 |
2004年 | 41篇 |
2003年 | 44篇 |
2002年 | 38篇 |
2001年 | 27篇 |
2000年 | 43篇 |
1999年 | 28篇 |
1998年 | 10篇 |
1997年 | 7篇 |
1996年 | 16篇 |
1995年 | 4篇 |
1992年 | 14篇 |
1991年 | 21篇 |
1990年 | 22篇 |
1989年 | 18篇 |
1988年 | 12篇 |
1987年 | 14篇 |
1986年 | 9篇 |
1985年 | 15篇 |
1984年 | 9篇 |
1983年 | 7篇 |
1982年 | 13篇 |
1981年 | 8篇 |
1980年 | 7篇 |
1979年 | 13篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1974年 | 6篇 |
1969年 | 5篇 |
1965年 | 4篇 |
1957年 | 5篇 |
1949年 | 3篇 |
排序方式: 共有1196条查询结果,搜索用时 15 毫秒
1.
Miguel A. Sanz Pau Montesinos Haesook T. Kim Guillermo J. Ruiz-Argüelles María S. Undurraga María R. Uriarte Lem Martínez Rafael H. Jacomo Homero Gutiérrez-Aguirre Raul A. M. Melo Rosane Bittencourt Ricardo Pasquini Katia Pagnano Evandro M. Fagundes Edo Vellenga Alexandra Holowiecka Ana J. González-Huerta Pascual Fernández Javier De la Serna Salut Brunet Elena De Lisa José González-Campos José M. Ribera Isabel Krsnik Arnold Ganser Nancy Berliner Raul C. Ribeiro Francesco Lo-Coco Bob L?wenberg Eduardo M. Rego 《Annals of hematology》2015,94(8):1347-1356
2.
Regional lens adenine nucleotide levels were determined in human and bovine lenses using sensitive HPLC techniques. In adult lenses of both species adenine nucleotide levels were highest in the anterior cortex (plus epithelium and capsule) followed by the posterior cortex (plus capsule) and lowest in the nucleus. With increasing lens age ATP level remained steady in the anterior cortex (plus epithelium and capsule) and nucleus, while those in the posterior cortex showed a tendency toward decrease. In both species, human as well as bovine, adenine nucleotide levels of the anterior cortex (plus epithelium and capsule) underwent early postmortem changes. Thus, immediate postmortem sampling as well as regional determinations are a must for detailed studies of lens nucleotide metabolism. 相似文献
3.
Multiclefted nuclei. A helpful feature for identification of intermediate trophoblastic cells in uterine curetting specimens. 总被引:1,自引:0,他引:1
Intermediate trophoblast is a distinct form of trophoblast, the presence of which in uterine curettings is considered a reliable indicator of intrauterine pregnancy even in the absence of chorionic villi. However, the appearance of intermediate trophoblastic cells have not been described in sufficiently specific terms to permit their reliable identification, and distinction from decidual cells can be difficult. We have noticed for some time that the intermediate trophoblastic cells often show multiple deep clefts in the nuclei, and the present study was performed to address the issue of whether this nuclear feature is reliable for their identification. We reviewed 242 uterine curettings of intrauterine pregnancy, documented by the presence of chorionic villi, and were able to find a distinct population of cells with large, hyperchromatic, multiclefted nuclei scattered in the decidua in 88% of the cases. In most instances, these cells produced a characteristic variegated pattern that was readily recognizable at low magnification. Positive immunostaining for cytokeratin (CAM 5.2) in these isolated cells within the decidua confirmed their trophoblastic nature. In contrast, multiclefted nuclei were absent in the 51 negative control cases, which included decidualized endocervical polyps (40 cases), uterine curettings from patients with tubal pregnancy (10 cases), and endometriosis with decidual change (one case). We conclude that intermediate trophoblastic cells can usually be reliably identified in curettings of intrauterine pregnancy by their characteristic nuclear multiclefting. 相似文献
4.
Spleen cells of a Biozzi HR mouse immunized with a bovine serum albumin-methotrexate conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells. Twenty-three monoclonal antibodies (MAbs), selected by indirect ELISA, were produced and partially characterized. Using methotrexate (MTX) and eight structurally related compounds, binding specificities of the MAbs were assessed by inhibition enzyme immunoassay. All the MAbs had very low affinity for folic acid and its analogs and for the major MTX metabolite 7-hydroxymethotrexate. Using a computer cluster analysis program based on the binding specificities, the MAbs were divided into three groups. The thirteen MAbs in group I recognized primarily the pteridine portion of the MTX molecule; the eight group II MAbs recognized the benzene ring as well as the pteridine structure. The two MAbs in group III poorly distinguished between the different parts of the MTX molecule. The apparent equilibrium association constants of the anti-MTX MAbs in groups I, II, and III ranged from 7 x 10(9) to 3 x 10(8) M-1 (except for 1 MAb), from 5 x 10(7) to 6 x 10(6) M-1 (except for 2 MAbs), and from 1 x 10(6) to 3.5 x 10(5) M-1, respectively. 相似文献
5.
6.
H Pau 《Klinische Monatsbl?tter für Augenheilkunde》1988,193(6):550-553
The three important types of age-related senile cataract, namely deep supranuclear cortical cataract, superficial subcapsular cortical cataract (sometimes associated with secondary nuclear cataract), and primary nuclear cataract, are arranged in a table which may be used for quick reference with regard to clinical features - slit lamp findings, age at onset of opacification, rate of progression, and related biochemistry (e.g., ATP content, glutathione content, and the cation pump (K+ greater than Na+] - as well as etiological features. The significance of the table is illustrated on the basis of an important example from the literature ("Sunlight and Human Cataracts"). 相似文献
7.
Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes. 总被引:9,自引:4,他引:9
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
B B Plikaytis G M Carlone C P Pau H W Wilkinson 《Journal of clinical microbiology》1987,25(11):2080-2084
In a previous study, all convalescent-phase sera from patients with culture-confirmed legionellosis reacted on immunoblots with a Legionella genus-wide 58-kilodalton (kDa) protein antigen (J.S. Sampson, B.B. Plikaytis, and H.W. Wilkinson, J. Clin. Microbiol. 23:92-99, 1986). The present study was done to immunologically characterize and determine the diagnostic relevance of this purified antigen. The antigen was precipitated from enriched cell extracts with ammonium sulfate and purified by high-pressure liquid chromatography. High-titered rabbit antiserum produced to the purified protein was used to show its presence on immunoblots in the 60-kDa range in 38 Legionella serogroups, representing 23 species, and in 39 non-Legionella bacteria. The antiserum was made specific for Legionella strains by sequential absorptions with Bordetella pertussis, Pseudomonas aeruginosa, and Pseudomonas fluorescens whole cells. Serum from legionellosis patients reacted with both specific and nonspecific epitopes. Results of indirect immunofluorescence experiments showed that neither specific nor nonspecific epitopes of the 60-kDa protein were surface exposed on Legionella cells and that cross-reactive epitopes were variably exposed on non-Legionella bacteria. The 60-kDa protein antigen should be useful in diagnostic tests for legionellosis if care is taken to expose cryptic epitopes and if the tests use or measure only the Legionella-specific epitopes. 相似文献
8.
Diagnosis of human immunodeficiency virus type 1 infection with different subtypes using rapid tests
Phillips S Granade TC Pau CP Candal D Hu DJ Parekh BS 《Clinical and diagnostic laboratory immunology》2000,7(4):698-699
We evaluated six rapid tests for their sensitivity and specificity in diagnosing human immunodeficiency virus type 1 (HIV-1) infection using 241 specimens (172 HIV-1 positive, 69 HIV-1 negative) representing different HIV-1 subtypes (A [n = 40], B [n = 47], C [n = 28], E [n = 42], and F [n = 7]). HIVCHEK, Multispot, RTD and SeroStrip were 100% sensitive and specific. Capillus failed to identify two of eight subtype C specimens (overall sensitivity of 98. 85%), while the SUDS test (the only test approved by the Food and Drug Administration) gave false-positive results for 5 of 69 seronegative specimens (specificity of 93.24%). Our results suggest that although rapid tests perform well in general, it may be prudent to evaluate a rapid test for sensitivity and specificity in a local population prior to its widespread use. 相似文献
9.
10.
The impact of heroin on frontal executive functions. 总被引:4,自引:0,他引:4
Charles W H Pau Tatia M C Lee Shui-fun F Chan 《Archives of clinical neuropsychology》2002,17(7):663-670
Our study examined the impact of heroin on frontal executive functioning in three cognitive domains, namely attention, impulse control, and mental flexibility and abstract reasoning. It was hypothesized that heroin addiction would lead to deficits in these three cognitive domains. Fifty-five participants, 30 heroin addicts, and 25 normal controls were invited to participate in this study. Each participant was individually interviewed for demographic data and tested by the selected neuropsychological measures. The findings indicate that heroin addiction has a negative effect on impulse control, while attention and mental flexibility/abstract reasoning ability were not affected. 相似文献