Contact lens disinfecting solutions antibacterial efficacy: comparison between clinical isolates and the standard ISO ATCC strains of Pseudomonas aeruginosa and Staphylococcus aureus

Purpose

To evaluate the disinfectant properties of the three multipurpose contact lens disinfecting solutions available in Iran, against clinical isolates and the standard ISO ATCC strains of Pseudomonas aeruginosaand Staphylococcus aureus, based on the international organization for standardization (ISO) 14729 guidelines.

Methods

Three multipurpose solutions that were tested were ReNu Multiplus, Solo Care Aqua and All-Clean Soft. The test solutions were challenged with clinical isolates and the standard strains of P. aeruginosa(ATCC 9027) and S. aureus(ATCC 6538), based on the ISO Stand-alone procedure for disinfecting products. Solutions were sampled for surviving microorganisms at manufacturer''s minimum recommended disinfection time. The number of viable organisms was determined and log reductions calculated.

Results

All of the three test solutions in this study provided a reduction greater than the required mean 3.0 logarithmic reduction against the recommended standard ATCC strains of P. aeruginosaand S. aureus. Antibacterial effectiveness of Solo Care Aqua and All-Clean Soft against clinical isolates of P. aeruginosaand S. aureuswere acceptable based on ISO 14729 Stand-alone test. ReNu MultiPlus showed a minimum acceptable efficacy against the clinical isolate of S. aureus, but did not reduce the clinical isolate by the same amount.

Conclusions

Although the contact lens disinfecting solutions meet/exceed the ISO 14729 Stand-alone primary acceptance criteria for standard strains of P. aeruginosaand S. aureus, their efficacy may be insufficient against clinical isolates of these organisms.     

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Comparative efficacies of contact lens disinfecting solutions against Pseudomonas aeruginosa

Purpose: The aim of the present study was to evaluate the disinfection properties of multipurpose contact lens disinfection solutions, based on the International Organization for Standardization (ISO) 14729 guidelines. Methods: Three lots of each of six multi‐purpose solutions were tested as follows: Renu MultiPlus, Solo Care Aqua, All‐Clean Soft, Contact All‐in one Advanced, Hippia, Ginza multi‐purpose solution and 0.9% normal saline solution were inoculated with the standard strain of Pseudomonas aeruginosa (ATCC 9027). The surviving bacteria were quantified at specified times. An average logarithm reduction in bacterial numbers at the manufacturer's minimum recommended disinfection time was determined and compared with the criteria for stand‐alone disinfection products for each multi‐purpose disinfection solution against each bacterial strain. Results: Renu MultiPlus, Solo Care, All‐Clean Soft and Contact All‐in one Advanced were effective in achieving a four logarithmic units reduction in the bacterial count; however, Renu MultiPlus and Solo Care Aqua resulted in the greatest reduction in bacterial numbers. The other solutions were not effective against Pseudomonas aeruginosa after specified times. Conclusions: Generally, Renu MultiPlus, Solo Care Aqua, All‐Clean Soft and Contact All‐in one Advanced meet the ISO 14729 guidelines for stand‐alone contact lens solutions.     

Antifungal efficacy of soft contact lens disinfecting solutions against Fusarium solani and Candida albicans

Purpose: The aim was to evaluate the disinfection properties of six multi‐purpose contact lens disinfection solutions (MPDS) available in Iran against Fusarium solani and Candida albicans, based on the International Organization for Standardization (ISO) 14729 guidelines. Methods: Three lots each of six multi‐purpose solutions, namely, Renu MultiPlus, Solo Care Aqua, All‐Clean soft, Contact All In One Advanced, Hippia, Ginza Multi‐Purpose Solution and 0.9% normal saline solution were inoculated with standard strains of Fusarium solani (ATCC 36031) and Candida albicans (ATCC 10231). Surviving fungi were determined by counting colony‐forming units on recovery plates at specified times. The mean log reduction in fungal numbers at the manufacturer's minimum recommended disinfection time was determined and compared with the criteria for stand‐alone disinfection products for each MPDS against each fungal strain. Results: Renu MultiPlus, Contact All In One Advanced and All‐Clean Soft were effective in achieving the mean 1.0 log reduction in fungal numbers, based on ISO 14729 stand‐alone primary acceptance criteria. Solo Care Aqua failed to achieve the ISO 14729 stand‐alone primary acceptance criteria for Candida albicans. The other solutions were not effective against test microorganisms after the specified times. Conclusions: There were differences in the rates of disinfection efficacy. Generally, Renu MultiPlus, Contact All In One Advanced and All‐Clean Soft exceeded the primary acceptance criteria of the ISO 14729 guidelines for stand‐alone contact lens solutions.